Chiba/Weekly
From 2007.igem.org
< Chiba(Difference between revisions)
(→Week4(9/9~9/15)) |
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Line 5: | Line 5: | ||
|- | |- | ||
!F/R | !F/R | ||
- | !207 | + | !FliC-207 |
- | !228 | + | !FliC-228 |
- | !240 | + | !FliC-240 |
- | !252 | + | !FliC-252 |
- | !267 | + | !FliC-267 |
|- | |- | ||
- | |207 | + | |FliC-207 |
|① | |① | ||
| | | | ||
Line 18: | Line 18: | ||
| | | | ||
|- | |- | ||
- | |228 | + | |FliC-228 |
|② | |② | ||
|③ | |③ | ||
Line 25: | Line 25: | ||
| | | | ||
|- | |- | ||
- | |240 | + | |FliC-240 |
|④ | |④ | ||
|⑤ | |⑤ | ||
Line 32: | Line 32: | ||
| | | | ||
|- | |- | ||
- | |252 | + | |FliC-252 |
|⑦ | |⑦ | ||
|⑧ | |⑧ | ||
Line 39: | Line 39: | ||
| | | | ||
|- | |- | ||
- | |267 | + | |FliC-267 |
|⑪ | |⑪ | ||
|⑫ | |⑫ | ||
Line 86: | Line 86: | ||
|1 | |1 | ||
|- | |- | ||
- | |207F | + | |FliC-207F |
|5 | |5 | ||
| | | | ||
Line 103: | Line 103: | ||
| | | | ||
|- | |- | ||
- | |207R | + | |FliC-207R |
|5 | |5 | ||
|5 | |5 | ||
Line 120: | Line 120: | ||
| | | | ||
|- | |- | ||
- | |228F | + | |FliC-228F |
| | | | ||
|5 | |5 | ||
Line 137: | Line 137: | ||
| | | | ||
|- | |- | ||
- | |228R | + | |FliC-228R |
| | | | ||
| | | | ||
Line 154: | Line 154: | ||
| | | | ||
|- | |- | ||
- | |240F | + | |FliC-240F |
| | | | ||
| | | | ||
Line 171: | Line 171: | ||
| | | | ||
|- | |- | ||
- | |240R | + | |FliC-240R |
| | | | ||
| | | | ||
Line 188: | Line 188: | ||
| | | | ||
|- | |- | ||
- | |252F | + | |FliC-252F |
| | | | ||
| | | | ||
Line 205: | Line 205: | ||
| | | | ||
|- | |- | ||
- | |252R | + | |FliC-252R |
| | | | ||
| | | | ||
Line 222: | Line 222: | ||
| | | | ||
|- | |- | ||
- | |267F | + | |FliC-267F |
| | | | ||
| | | | ||
Line 239: | Line 239: | ||
|5 | |5 | ||
|- | |- | ||
- | |267R | + | |FliC-267R |
| | | | ||
| | | | ||
Line 342: | Line 342: | ||
|- | |- | ||
|} | |} | ||
- | |||
- | |||
== Week2(8/24~8/31) == | == Week2(8/24~8/31) == | ||
Line 352: | Line 350: | ||
== Week4(9/9~9/15) == | == Week4(9/9~9/15) == | ||
+ | *Swaming test | ||
+ | #FliC-267His-RBSプラスミドをJW1908(KEIO⊿FliC)にTransformation(Positive Control=ASKA,Negative Control=KEIO-JW1908にFliC-267HisをTransformしたもの) | ||
+ | #LB培地で37℃12h2ml培養 | ||
+ | #トリプトン培地(Agar0.25%)の中央に1μinoculate | ||
+ | #30℃、24-48h,Taxis | ||
+ | #Taxis ling を観察 | ||
+ | *'''Sample''' | ||
+ | [[Image:swam4-2-2.jpg]] | ||
== Week5(9/16~9/22) == | == Week5(9/16~9/22) == |
Latest revision as of 02:48, 12 September 2007
Contents |
Week1(8/16~8/23)
Monday,August,17,2007
- pUC-FliCのPCR
F/R | FliC-207 | FliC-228 | FliC-240 | FliC-252 | FliC-267 |
---|---|---|---|---|---|
FliC-207 | ① | ||||
FliC-228 | ② | ③ | |||
FliC-240 | ④ | ⑤ | ⑥ | ||
FliC-252 | ⑦ | ⑧ | ⑨ | ⑩ | |
FliC-267 | ⑪ | ⑫ | ⑬ | ⑭ | ⑮ |
Recipe(単位:μ) | ① | ② | ③ | ④ | ⑤ | ⑥ | ⑦ | ⑧ | ⑨ | ⑩ | ⑪ | ⑫ | ⑬ | ⑭ | ⑮ |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
pUC19-FliC | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
FliC-207F | 5 | ||||||||||||||
FliC-207R | 5 | 5 | 5 | 5 | 5 | ||||||||||
FliC-228F | 5 | 5 | |||||||||||||
FliC-228R | 5 | 5 | 5 | 5 | |||||||||||
FliC-240F | 5 | 5 | 5 | ||||||||||||
FliC-240R | 5 | 5 | 5 | ||||||||||||
FliC-252F | 5 | 5 | 5 | 5 | |||||||||||
FliC-252R | 5 | 5 | |||||||||||||
FliC-267F | 5 | 5 | 5 | 5 | 5 | ||||||||||
FliC-267R | 5 | ||||||||||||||
10×Buffer | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 |
dNTPmix | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 |
Vent | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
dH2O | 68 | 68 | 68 | 68 | 68 | 68 | 68 | 68 | 68 | 68 | 68 | 68 | 68 | 68 | 68 |
Total | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
Week2(8/24~8/31)
Week3(9/1~9/8)
Week4(9/9~9/15)
- Swaming test
- FliC-267His-RBSプラスミドをJW1908(KEIO⊿FliC)にTransformation(Positive Control=ASKA,Negative Control=KEIO-JW1908にFliC-267HisをTransformしたもの)
- LB培地で37℃12h2ml培養
- トリプトン培地(Agar0.25%)の中央に1μinoculate
- 30℃、24-48h,Taxis
- Taxis ling を観察
- Sample