Melbourne/Lab Notebook Weeks 13-16

From 2007.igem.org

(Difference between revisions)
(Week 13)
(Week 13)
Line 42: Line 42:
*# Cph8 A X/P
*# Cph8 A X/P
*# Cph8 B X/P
*# Cph8 B X/P
 +
 +
 +
-All had desired insert except for lanes 8-11 (OmpR-C1-reporter) in Gel 1 and lanes 4, 5 (CpH8) in Gel 2.
 +
 +
*Gel purified:
 +
*# [[Melbourne/BBa_I15008|P2 21A]]  E/X
 +
*# [[Melbourne/BBa_I15009|P2 21C]]  E/X
 +
*# Death-CompA E/X
 +
*# Death-ComP E/X
 +
*# [[Melbourne/BBa_J61035|P4 8J]] A E/S
 +
*# [[Melbourne/BBa_J61035|P4 8J]] B E/S
 +
 +
*[[Melbourne/Ligation Protocol|Ligated]] using 1hour ligation method at room temperature:
 +
**[[Melbourne/BBa_J61035|P4 8J]] E/S + [[Melbourne/BBa_I15008|P2 21A]]  E/X
 +
**[[Melbourne/BBa_J61035|P4 8J]] E/S + [[Melbourne/BBa_I15009|P2 21C]]  E/X
 +
**[[Melbourne/BBa_J61035|P4 8J]] E/S + Death-CompA E/X
 +
**[[Melbourne/BBa_J61035|P4 8J]] E/S + Death-ComP E/X
 +
 +
*[[Melbourne/Transformation Protocol|Transformed]] above and plated.
Line 47: Line 66:
</b>
</b>
</font><BR>
</font><BR>
 +
 +
*[[Melbourne/Growing up cells|Liquid cultured]] 4 colonies each from transformants of 17/9 (2 from Death-ComA).
Line 52: Line 73:
</b>
</b>
</font><BR>
</font><BR>
 +
 +

Revision as of 12:38, 9 October 2007

<Return to Lab notebook> <team home page>

Contents

Week 13

16 Sept 2007


17 Sept 2007

Gel1:

    1. DNA ladder
    2. P17OA E/H
    3. P17OB E/H
    4. P1 5G A E/H
    5. P1 5G B E/H
    6. Omp-C1-reporter A X/P
    7. Omp-C1-reporter B X/P
    8. Omp-C1-reporter C X/P
    9. Omp-C1-reporter D X/P
    10. P2 21A A X/P
    11. P2 21A B X/P
    12. P2 21C A X/P
    13. P2 21C B X/P
    14. Death-ComA 5min ligation A X/P
    15. Death-ComA 5min ligation B X/P
    16. Death-ComA overnight ligation A X/P
    17. Death-ComA overnight ligation B X/P
    18. DNA ladder

Gel2:

    1. DNA ladder
    2. Death-ComP A X/P
    3. Death-ComP B X/P
    4. Cph8 A X/P
    5. Cph8 B X/P


-All had desired insert except for lanes 8-11 (OmpR-C1-reporter) in Gel 1 and lanes 4, 5 (CpH8) in Gel 2.


18 Sept 2007

  • Liquid cultured 4 colonies each from transformants of 17/9 (2 from Death-ComA).


19 Sept 2007



20 Sept 2007


21 Sept 2007


22 Sept 2007

Week 14

23 Sept 2007


24 Sept 2007


25 Sept 2007


26 Sept 2007

  • Miniprepped the following
    • TetR-RBS-P2,21A-ter A,B,C and D
    • TetR-RBS-ComP-ter A,B,C and D
    • GenRBS-ComA-ter A,B,C and D
    • cI-L3 A,B,C and D

27 Sept 2007


28 Sept 2007


29 Sept 2007


Week 15

30 Sept 2007


1 Oct 2007


2 Oct 2007


3 Oct 2007


4 Oct 2007


5 Oct 2007


6 Oct 2007


Week 16

7 Oct 2007


8 Oct 2007


9 Oct 2007


10 Oct 2007


11 Oct 2007


12 Oct 2007


13 Oct 2007