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-	__NOTOC__	+	{{Valencia/Cabecera}}
-	<center>[https://2007.igem.org/Valencia https://static.igem.org/mediawiki/2007/0/0a/BanneriGEM2007.jpg]</center>	+
-	==The Biological Comparator (for a more detailed description, you can visit our [http://www.igem.upv.es/igem07/ local wiki])==	+	During the brainstroming, an idea came up: mimetize an [[Valencia/Electronic comparator|electronic comparator]] on a biologcal device. It works with repressors, so it may sound familiar to most of us.
-	The objective of our project is to create a device which behavior resembles an electronic comparator.	+	'''What:'''
-	The comparator works analyzing two input signals, like two proteins or metabolites, and responses with a given output, as fluorescence, related to the concentration of the input signal. Each input has an associated fluorescence color.	+	Two inputs enter the system, a genetic circuit compares both signals and amplifies the higher one.
-	The interesting part is that differences of the output signals become amplified whenever a slight disparity between the initial concentrations of the input signals occurs.	+	'''How:'''
-	If the inputs are equally sensed, both fluorescence have equal concentrations, but whenever one of the input’s level is above the other, this fluorescence becomes greater and the other one gets inhibited. This way, we get an amplification at the output level of a small difference at the input level.	+
-	One of the principal reason why we decided to develop this project was the potential modularity of its design. Just by changing the promoters this device could be used for compare whatever you want.	+	So easy, I would be quite surprised if we don't find any drawback.
-	The comparator device could be implemented in more complex systems, like a concentration stabilizer; a quality checker, verifying the right levels of an input; and could be designed a system with two or more comparators interconnected.	+	Two different promoters that act as sensors of different inputs and two repressors that inhibit each other.
		+
		+	#We have two promoters that 'sense' something: psensorA and psensorB
		+	#We have two promoters repressed: pLacI and ptet
		+	#We also have two CDS of the repressors: lacI and tetR
		+	#And, finally, we have the outputs: CFP and YFP
		+
		+	The system would be:
		+
		+	[[Image:VComparator-fluor.jpg|500px]]
		+
		+	So, if we have equal amount of whatever A and B, there will be equal amounts of both repressors and, so, equal amounts of CFP and YFP. If some input A is sensibly higher than the B, we will have more tetR that will inhibit ptet and we will have less YFP
		+
		+	'''What for:'''
		+
		+	We would have a device that would clear the noise of a system, (only reducing it to zero at infinite time).
		+	It can be used in other projects, as it implies only two genes and 4 promoters...
		+
		+	'''Problems:'''
		+	* Will a gene driven by two different promoters work well enough?? ETH tried it with some results, why not give it a try?
		+	* It's a small project, but it can be coupled to some other project...
		+	* We still have to find some new BioBricks to build, as almost every single construction would be on the iGEM plate
		+
		+	'''Developed by:''' Arnau, Pablo and Javi

---

Latest revision as of 15:06, 26 October 2007

Summary The idea Promoter calibrator Biological comparator Electronic comparator Biological controller Lab Work Silico Work Results Adversities

During the brainstroming, an idea came up: mimetize an electronic comparator on a biologcal device. It works with repressors, so it may sound familiar to most of us.

What:

Two inputs enter the system, a genetic circuit compares both signals and amplifies the higher one.

How:

So easy, I would be quite surprised if we don't find any drawback.

Two different promoters that act as sensors of different inputs and two repressors that inhibit each other.

1. We have two promoters that 'sense' something: psensorA and psensorB
2. We have two promoters repressed: pLacI and ptet
3. We also have two CDS of the repressors: lacI and tetR
4. And, finally, we have the outputs: CFP and YFP

The system would be:

So, if we have equal amount of whatever A and B, there will be equal amounts of both repressors and, so, equal amounts of CFP and YFP. If some input A is sensibly higher than the B, we will have more tetR that will inhibit ptet and we will have less YFP

What for:

We would have a device that would clear the noise of a system, (only reducing it to zero at infinite time). It can be used in other projects, as it implies only two genes and 4 promoters...

Problems:

• Will a gene driven by two different promoters work well enough?? ETH tried it with some results, why not give it a try?
• It's a small project, but it can be coupled to some other project...
• We still have to find some new BioBricks to build, as almost every single construction would be on the iGEM plate

Developed by: Arnau, Pablo and Javi