From 2007.igem.org

Contents 1 5:30 Action points from last meeting: 2 5:45 Status Reports: (5 minutes each including any discussion) 3 6.15 New Agenda Items: 4 7:30 End 5 Igem Administrative Timetable: 6 Documentation wiki status:

5:30 Action points from last meeting:

• Alisa& Pat: Wiki "Parts_used status" up to date? -(outstanfding from 19/7/07)
• Jan: Lecture Timetables spread sheet to be emailed to all
• Pat & Alisa: Plan for red light receptor on wiki
• Alisa, Paul & Jan: Review blue light theory
• Jan:Theory for blue light on wiki
• Jan:ComP fusion to be ordered.
• Jan:Production of sequence for blue light receptor? " -designed
• Jan:Plan for blue light on wiki by 17th August
• Jan:<b/>Order red and blue filters.
• <b>Paul: Status of #BBa-M30109 & #BBa-V1012 lost in transit?
• Jan: Production of sequence for blue light receptor? " -designed
• Craig: Alkaline lysis technique for miniprep to be documented
• Cheng: Daisy to produce competent cells of DB3.1 and Enz- strains.

5:45 Status Reports: (5 minutes each including any discussion)

• Alisa: Ligation experiments progress.
  • Problem: 1.2KB genes between biobrick forward & reverse primers not being visualised in colony PCR with 1.5min extn time. (300bp fragments do amplify)
    • - craig same ligation worked fine [no BSA, no gel purification, no Alkaline phosphatase used]
    • - craig very high 10mg/ml DNA yield from miniprep using alkaline lysis technique.
    • - Alisa: doesn't believe it is worth investigating any further except as experiments are done.
• Pat & Alisa: Red light receptor progress/design/plan- needs?
  • phase 1 osmolarity test of YFP under enz
  • phase 2 Red light driving YFP
  • Phil would like to see lacZ coliphotography parts available for tests due to higher sensitivity of enzyme output.
• Jan: Blue light receptor design/plan - needs?
  • Phil sugests using prism for optimal colour discovery before ordering filters, Projector as source.
  • Review to be cionducted with Jan, Paul & Alisa
  • No need to order any apperatus yet.
• Phil: Gas vesicles progress/design/plan - needs?
  • Plan up to basic biobrick complete,
  • First mutagenesis in progress using complementry primers hand designed, and one overlapping and one computer generated using stratagene program.
  • Plan for support parts/test harness required.
  • Lac promotor and antibiotic death plasmids required.
• Kasia: Documentation progress vs last report/ new problems/ needs.-No report

6.15 New Agenda Items:

• Phil: Individuals desired time commitment:
• Alisa: To Invite Chris to join team as full member.
• Phil: Administrative Timetable: (11 Weeks to go)
• Next meeting: <b/> 10th August (Friday) 5.30PM agreed but latter changed to ) 5:15PM.

7:30 End

Igem Administrative Timetable:

1 September	Final team rosters due.
1 September	Jamboree attendance fees due $100USD per team member.
26 October	Project and part documentation due DNA received USA.
26 October	BioBrick Part DNA received by the Registry.
3-4 November	iGEM Competition Jamboree, MIT, USA.

.

Documentation wiki status:

• • Priority should be on plans and experimental report analysis and conclusions-----Alisa .
  • If only Pat & Alisa doing lab work not much documentation needed.
  • Equipement:
    • MissingLas3000 method (in lab notebook)
    • Missing DNA measurement protocol (eppendorf)
  • Reagents pages incomplete:
    • Some location pictures not finished or uploaded.
    • Some newer reagents not entered.
  • Secondary reagents pages not made:
    • LB, Plates, TAE , NZY+ Broth
  • Protocol part:
    • No connections from Protocols to reagents.
    • Ligation protocol missing
  • Lab notebook:
    • Format uses too many lines to see connections between days.
    • Latter part missing links
    • Can table of contents be restricted to date level?
  • Experimental reports:
    • Lacks discussion/conclusion
    • Ligation experiment missing
    • Lattest few gells not marked up and entered.
  • Project overview link broken
  • Background:
    • GFP missing ,
    • Red photo sensor to GFP test harness missing.
  • Plan: Only Gas vesicles plan entered.

Individual	Desired time committment
Pat	About 4h/wk Monday afternoon or Tuesday morning
Alisa	About 4h/wk - Thursday afternoon
Jan	About 10h/pw - flexible weekday mornings
Phil	About 18h/pw - Thursday morning, Friday, Saturday or Sunday,