Paris/Elementary rules

From 2007.igem.org

In Bacteria:

 DAP + Export -> DAP_Export                     // Diffusion  (from a pump)
 DAP_Import -> DAP + Import                     //
 Cre -> .                                       // Degradation (inside)
 DAP -> .                                       //
 DAPAp + DAP <-> DAPAp_i                        // Promotor Activity
 DAPAp -> DAPAp + Cre                           //
 DAPAp_i -> DAPAp_i + Cre   (low probability)   //
 LOXP_Box + 4Cre -> DAP_Box + 4Cre              // Differentiation
 
 DAP_Box -> DAP_Box + DAP                       // DAP Production 



Out Bacteria:

 x:Bact \/ LOXP_Box -> x1 + x2                  // Division* (under control of Ftsz A or not ?)
 DAP + (x \/ Import) -> DAP_Import :: x         // Diffusion
 x \/ DAP_Export -> DAP + (Export :: x)         //
 DAP -> .                                       // DAP Degradation  (outside)
  • Doubling of the genetic material + random repartition of the others molecules between the two "daughters"



notes:


\ /: means "with" LOXP_Box: is inside the germinal line

DAP_Box: is the LOXP_Box recombined, is inside the somatic line

- there is a low probability, that DAP is able to act alone.

- we don't know yet, if CRE need 4 molecules or if it is a cooperative fixation

- in the division, we have take care of the DAP affinity for the promotor.

- dependence of the germinal line for DAP:

   - if hight concentration (does she died ?)
   - if low concentration ( slow down of the division)--> death's dependence for the level of the DAP