Paris/October 17
From 2007.igem.org
< Paris
| Digestion Products | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Number | Product Name | Matrix Name | Enzyme 1 | Enzyme 2 | Size | Description | |||
| D94 | PacI[ptet>>lox71-gfpT-lox66-mrfp]SmaI | P15 | PacI | XmaI | ptet>>lox71-gfpT-lox66-mrfp P15 PCR product digested by PacI-SmaI for cloning into pY-2C-IntC | ||||
| D95 | PacI[pY-2C-IntC]SmaI | pY-2C-IntC plasmid | PacI | XmaI | pY-2C-IntC digested for ptet>>lox71-gfpT-lox66-mrfp cloning | ||||
| D82 | frt1-CmR-frt2 FI | P16 | EcoRI | SpeI | frt1-CmR-frt2 PCR product digested for use as a FI | ||||
| D83 | frt1-CmR-frt2 BI | P16 | XbaI | PstI | frt1-CmR-frt2 PCR product digested for use as a BI | ||||
| D84 | frt1-CmR-frt2 BBi | P16 | EcoRI | PstI | frt1-CmR-frt2 PCR product digested for cloning as a BB | ||||
Unfortunatly, D82 digestion reaction gave an unexpected result with a band at 500bp
