Peking sandbox

There are days left until the Jamboree!

Hop Count with Conjugation Hop counting is a cell-cell communication system in which the signals transfered among the cells record the number of times of transfer. We decided to use conjugation as our one to one cell-cell communication system and plasmids as the vector for the transfered signal. With the rolling-cycle replication of conjugation, independent tandem oriT (BBa_J01002 and more) pairs can be inserted into the same plasmids, losing one tandem region at each single conjugation event. Sequential conjugation and deletion of the different oriT pairs can be achieved by fine control of the expression of the cis-acting elements of the independent conjugation systems. Besides intellectual interests, these systems are the core parts of controllable and encoded local cell-cell communication, which can be used in potential pattern formation and material weaving applications.
Push-on-push-off Switch We propose to construct a gene regulatory circuit in E. coli which functions as a push-on/push-off switch. The immediate output of such circuit is determined by the input signal and the internal state of the circuit. It is designed to response to low dose UV irradiation. Under intermittent irradiation, two reporter genes are expected to be induced alternately.

Team

Meetings

• iGEM promotion meeting
• iGEM PKU prep meeting
• Journal Club 01 | 02 | 03 | 04 | 05 | 06 | 07 | 08 | 09 | 10 | 11 | 12 | 13 | 17

Brainstorming
Some of our ideas are listed here

Project

Hop Count with Conjugation

• Project Proposal
• Project Presentation
• Road Map
• Related Articles

Push-on-push-off Switch

• Project proposal
• Project presentation
• Road Map
• Related Articles

Brainstorming
Some of our ideas are listed here

People

Advisors
[http://www.bio.pku.edu.cn/collegereview/scholarinfo.jsp?name=wangyp Wang Yiping]
Li Fangting
[http://ctb.pku.edu.cn/main/cn/graduation/graduation-daoshijianjie-ouyangqi.htm Ouyang Qi]
[http://linux2.ipc.pku.edu.cn/ Lai Luhua]

Graduates
Liu Xili
Lou Chunbo
Jiang Lingli
Ni Ming

Undergrads
Wang Mingcong
Chen Congyi
Chen Daizhuo
Ma Tao
Ma Haisu
Lu Dan
Zheng Qinsi
Liu Chang
Qu Mingzhi
Yang Yifan
Ren Zhe
Yu Tao
Xu Anting
Liu Ting

Undergards->Grads
Chen Xiaoyue
Li Tuo
Yang Le