Wisconsin/Digestion
From 2007.igem.org
Endonuclease Digestion
- 10uL ddH2O
- 5uL DNA
- 2uL 10x buffer
- 2uL 1:10 dilution of BSA soln
- 0.5uL Enzyme 1
- 0.5uL Enzyme 2
Incubate at 37C for 2-4 hours
Use [http://www.promega.com/guides/re_guide/RESearch.asp this website] for restriction enzyme and [http://www.promega.com/guides/re_guide/RESearch.asp?search=buffer this site] for checking buffer compatibility.
