Glasgow/Wetlab
From 2007.igem.org
(→03/07) |
|||
| Line 8: | Line 8: | ||
=== 03/07 === | === 03/07 === | ||
Maija and Christine prepared LB broth and LB agar with Protocol 1. Made 4 batches of plates, 2 with kanamycin and 2 with carbenicillin. | Maija and Christine prepared LB broth and LB agar with Protocol 1. Made 4 batches of plates, 2 with kanamycin and 2 with carbenicillin. | ||
| + | |||
| + | === 04/07 === | ||
| + | 1 All wetlab researched BioBricks. | ||
| + | 2 Reporter constructs and mini-Tn5 stocks looked out. | ||
| + | 3 Streaked the following: P. putida PAW 340 pJAK14 Carb. plate | ||
| + | Tn5 lux AB Carb. plate | ||
| + | Mini-Tn5 lux AB Carb. plate | ||
| + | P. Fluorescens NCIMB 9815 Carb. plate | ||
| + | P. putida KT2440 LB | ||
| + | |||
| + | JM109 pBluescript 5k+ Carb. plate | ||
| + | Mini-Tn5 Tc Carb. Plate | ||
| + | pQF52 Carb. plate | ||
| + | P. Fluorescens 9815 LB | ||
| + | E. coli pJAK14 Km plate | ||
| + | |||
| + | Il DntR in pOF52 Carb. plate | ||
| + | pUCINR in Ω strain C Carb. plate | ||
| + | pGLTUR Carb. plate | ||
| + | Mini-Tn5 Kan Carb. plate | ||
| + | Mini-Tn5 Sm/Sp Carb. plate | ||
| + | |||
| + | Mini-Tn5 1cc2 Carb. plate | ||
| + | E. coli Sa1 LB | ||
| + | DmpR #24 Carb. plate | ||
| + | Mini-Tn5 lac 32 in E. coli 517 Carb. plate | ||
| + | Mini-Tn5 Tc Carb. Plate | ||
| + | |||
| + | Mini-Tn5 Cm Carb. plate | ||
| + | DmpR WT Carb. plate | ||
| + | E.coli sm 10 pESD15 Tn5 GFP Carb. plate | ||
| + | pUJ8 Carb. Plate | ||
Revision as of 16:23, 19 July 2007
Week 1
03/07
Maija and Christine prepared LB broth and LB agar with Protocol 1. Made 4 batches of plates, 2 with kanamycin and 2 with carbenicillin.
04/07
1 All wetlab researched BioBricks. 2 Reporter constructs and mini-Tn5 stocks looked out. 3 Streaked the following: P. putida PAW 340 pJAK14 Carb. plate Tn5 lux AB Carb. plate Mini-Tn5 lux AB Carb. plate P. Fluorescens NCIMB 9815 Carb. plate P. putida KT2440 LB
JM109 pBluescript 5k+ Carb. plate Mini-Tn5 Tc Carb. Plate pQF52 Carb. plate P. Fluorescens 9815 LB E. coli pJAK14 Km plate
Il DntR in pOF52 Carb. plate pUCINR in Ω strain C Carb. plate pGLTUR Carb. plate Mini-Tn5 Kan Carb. plate Mini-Tn5 Sm/Sp Carb. plate
Mini-Tn5 1cc2 Carb. plate E. coli Sa1 LB DmpR #24 Carb. plate Mini-Tn5 lac 32 in E. coli 517 Carb. plate Mini-Tn5 Tc Carb. Plate
Mini-Tn5 Cm Carb. plate DmpR WT Carb. plate E.coli sm 10 pESD15 Tn5 GFP Carb. plate pUJ8 Carb. Plate
