Glasgow/Wetlab

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== Week 1 ==
== Week 1 ==
=== 03/07 ===
=== 03/07 ===
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Maija and Christine prepared LB broth and LB agar with Protocol 1.  Made 4 batches of plates, 2 with kanamycin and 2 with carbenicillin.
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#Maija and Christine prepared LB broth and LB agar with Protocol 1.  Made 4 batches of plates, 2 with kanamycin and 2 with carbenicillin.
=== 04/07 ===
=== 04/07 ===

Revision as of 16:28, 19 July 2007

PROTOCOLSREFERENCES

Week 1

03/07

  1. Maija and Christine prepared LB broth and LB agar with Protocol 1. Made 4 batches of plates, 2 with kanamycin and 2 with carbenicillin.

04/07

  1. All wetlab researched BioBricks.
  2. Reporter constructs and mini-Tn5 stocks looked out.
  3. Streaked the following:
    1. P. putida PAW 340 pJAK14 Carb. plate
    2. Tn5 lux AB Carb. plate
    3. Mini-Tn5 lux AB Carb. plate
    4. P. Fluorescens NCIMB 9815 Carb. plate
    5. P. putida KT2440 LB
    6. JM109 pBluescript 5k+ Carb. plate
    7. Mini-Tn5 Tc Carb. Plate
    8. pQF52 Carb. plate
    9. P. Fluorescens 9815 LB
    10. E. coli pJAK14 Km plate
    11. Il DntR in pOF52 Carb. plate
    12. pUCINR in Ω strain C Carb. plate
    13. pGLTUR Carb. plate
    14. Mini-Tn5 Kan Carb. plate
    15. Mini-Tn5 Sm/Sp Carb. plate
    16. Mini-Tn5 1cc2 Carb. plate
    17. E. coli Sa1 LB
    18. DmpR #24 Carb. plate
    19. Mini-Tn5 lac 32 in E. coli 517 Carb. plate
    20. Mini-Tn5 Tc Carb. Plate
    21. Mini-Tn5 Cm Carb. plate
    22. DmpR WT Carb. plate
    23. E.coli sm 10 pESD15 Tn5 GFP Carb. plate
    24. pUJ8 Carb. Plate
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