Glasgow/Wetlab

From 2007.igem.org

(Difference between revisions)
(→04/07)
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#Reporter constructs and mini-Tn5 stocks looked out.
#Reporter constructs and mini-Tn5 stocks looked out.
#Streaked the following:
#Streaked the following:
-
***P. putida PAW 340 pJAK14 Carb. plate
+
##P. putida PAW 340 pJAK14 (Carb. plate)
-
##Tn5 lux AB Carb. plate
+
##Tn5 lux AB (Carb. plate)
-
##Mini-Tn5 lux AB Carb. plate
+
##Mini-Tn5 lux AB (Carb. plate)
-
##P. Fluorescens NCIMB 9815 Carb. plate
+
##P. Fluorescens NCIMB 9815 (Carb. plate)
-
##P. putida KT2440 LB
+
##P. putida KT2440 (LB)
-
##JM109 pBluescript 5k+ Carb. plate
+
##JM109 pBluescript 5k+ (Carb. plate)
-
##Mini-Tn5 Tc Carb. Plate
+
##Mini-Tn5 Tc (Carb. Plate)
-
##pQF52 Carb. plate
+
##pQF52 (Carb. plate)
-
##P. Fluorescens 9815 LB
+
##P. Fluorescens 9815 (LB)
-
##E. coli pJAK14 Km plate
+
##E. coli pJAK14 (Km plate)
-
##Il DntR in pOF52 Carb. plate
+
##Il DntR in pOF52 (Carb. plate)
-
##pUCINR in Ω strain C Carb. plate
+
##pUCINR in Ω strain C (Carb. plate)
-
##pGLTUR Carb. plate
+
##pGLTUR (Carb. plate)
-
##Mini-Tn5 Kan Carb. plate
+
##Mini-Tn5 Kan (Carb. plate)
-
##Mini-Tn5 Sm/Sp Carb. plate
+
##Mini-Tn5 Sm/Sp (Carb. plate)
-
##Mini-Tn5 1cc2 Carb. plate
+
##Mini-Tn5 1cc2 (Carb. plate)
-
##E. coli Sa1 LB
+
##E. coli Sa1 (LB)
-
##DmpR #24 Carb. plate
+
##DmpR #24 (Carb. plate)
-
##Mini-Tn5 lac 32 in E. coli 517 Carb. plate
+
##Mini-Tn5 lac 32 in E. coli 517 (Carb. plate)
-
##Mini-Tn5 Tc Carb. Plate
+
##Mini-Tn5 Tc (Carb. Plate)
-
##Mini-Tn5 Cm Carb. plate
+
##Mini-Tn5 Cm (Carb. plate)
-
##DmpR WT Carb. plate
+
##DmpR WT (Carb. plate)
-
##E.coli sm 10 pESD15 Tn5 GFP Carb. plate
+
##E.coli sm 10 pESD15 Tn5 GFP (Carb. plate)
-
##pUJ8 Carb. Plate
+
##pUJ8 (Carb. Plate)

Revision as of 16:32, 19 July 2007

PROTOCOLSREFERENCES

Week 1

03/07

  1. Maija and Christine prepared LB broth and LB agar with Protocol 1. Made 4 batches of plates, 2 with kanamycin and 2 with carbenicillin.

04/07

  1. All wetlab researched BioBricks.
  2. Reporter constructs and mini-Tn5 stocks looked out.
  3. Streaked the following:
    1. P. putida PAW 340 pJAK14 (Carb. plate)
    2. Tn5 lux AB (Carb. plate)
    3. Mini-Tn5 lux AB (Carb. plate)
    4. P. Fluorescens NCIMB 9815 (Carb. plate)
    5. P. putida KT2440 (LB)
    6. JM109 pBluescript 5k+ (Carb. plate)
    7. Mini-Tn5 Tc (Carb. Plate)
    8. pQF52 (Carb. plate)
    9. P. Fluorescens 9815 (LB)
    10. E. coli pJAK14 (Km plate)
    11. Il DntR in pOF52 (Carb. plate)
    12. pUCINR in Ω strain C (Carb. plate)
    13. pGLTUR (Carb. plate)
    14. Mini-Tn5 Kan (Carb. plate)
    15. Mini-Tn5 Sm/Sp (Carb. plate)
    16. Mini-Tn5 1cc2 (Carb. plate)
    17. E. coli Sa1 (LB)
    18. DmpR #24 (Carb. plate)
    19. Mini-Tn5 lac 32 in E. coli 517 (Carb. plate)
    20. Mini-Tn5 Tc (Carb. Plate)
    21. Mini-Tn5 Cm (Carb. plate)
    22. DmpR WT (Carb. plate)
    23. E.coli sm 10 pESD15 Tn5 GFP (Carb. plate)
    24. pUJ8 (Carb. Plate)
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