Glasgow/Wetlab/Gels
From 2007.igem.org
(Difference between revisions)
(→Gels) |
(→Gels) |
||
Line 46: | Line 46: | ||
11.neg control<br> | 11.neg control<br> | ||
|[[Image:10thAug gel 2.JPG|200px]] | |[[Image:10thAug gel 2.JPG|200px]] | ||
+ | |- | ||
+ | |[[Glasgow/Wetlab/Week7#Monday 13th August 2007|Mon 13th Aug 2007 (4)]] | ||
+ | |PCR of genes (*a→g*), (*a→d*), and (*d→g*). | ||
+ | |1A:(*a→g*) <br> | ||
+ | 1B:(*a→g*) <br> | ||
+ | 1C:(*a→g*) <br> | ||
+ | 2A:(*a→g*) <br> | ||
+ | 2B:(*a→d*) <br> | ||
+ | 2C:(*d→g*) <br> | ||
+ | |[[Image:2007-08-13 12hr 43min.JPG|200px]] | ||
|- | |- | ||
|[[Glasgow/Wetlab/Week7#Friday 17th August 2007|Fri 17th Aug 2007 (2-4)]] | |[[Glasgow/Wetlab/Week7#Friday 17th August 2007|Fri 17th Aug 2007 (2-4)]] |
Revision as of 12:31, 28 August 2007
Glasgow Main Page | Back To Wetlab Log
Gels
Gels are labelled with numbers for rows and letters for columns.
Date and Reference | Description | Key | Gel |
---|---|---|---|
Mon 13th Aug 2007 (4) | PCR of genes (*a→g*), (*a→d*), and (*d→g*). | 1A:(*a→g*) 1B:(*a→g*) | |
Fri 10th Aug 2007 (2) | Colony PCR after ligating (*m*), (*s*), DntR and Pu with the construction vectors 3/20G and 4/6B. | 1.(*m*) 2.(*m*) | |
Fri 10th Aug 2007 (2) | Colony PCR after ligating (*m*), (*s*), DntR and Pu with the construction vectors 3/20G and 4/6B. | 1.(*s*) 2.(*s*) | |
Mon 13th Aug 2007 (4) | PCR of genes (*a→g*), (*a→d*), and (*d→g*). | 1A:(*a→g*) 1B:(*a→g*) | |
Fri 17th Aug 2007 (2-4) | PstI digested minipreps of (*m*) and (*s*) in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis. |