Glasgow/Wetlab/Gels

(Difference between revisions)

Revision as of 12:31, 28 August 2007

Gels are labelled with numbers for rows and letters for columns.

Date and Reference	Description	Key
Fri 10th Aug 2007 (2)	Colony PCR after ligating (m), (s), DntR and Pu with the construction vectors 3/20G and 4/6B.	1.(m) 2.(m) 3.(s) 4.(s) 5.(s) 6.(s) 7.neg control 8.neg control 9.neg control
Fri 10th Aug 2007 (2)	Colony PCR after ligating (m), (s), DntR and Pu with the construction vectors 3/20G and 4/6B.	1.(s) 2.(s) 3.neg control 4.DntR 5.DntR 6.Pu 7.Pu 8.neg control 9.neg control 10.neg control 11.neg control
Mon 13th Aug 2007 (4)	PCR of genes (a→g), (a→d), and (d→g).	1A:(a→g) 1B:(a→g) 1C:(a→g) 2A:(a→g) 2B:(a→d) 2C:(d→g)
Fri 17th Aug 2007 (2-4)	PstI digested minipreps of (m) and (s) in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis.

@@ Line 8: / Line 8: @@
 !Key
 !Gel
-|-
-|[[Glasgow/Wetlab/Week7#Monday 13th August 2007|Mon 13th Aug 2007 (4)]]
-|PCR of genes (*a→g*), (*a→d*), and (*d→g*).
-|1A:(*a→g*) <br>
-B:(*a→g*) <br>
-C:(*a→g*) <br>
-A:(*a→g*) <br>
-B:(*a→d*) <br>
-C:(*d→g*) <br>
-|[[Image:2007-08-13 12hr 43min.JPG|200px]]
 |-
 |[[Glasgow/Wetlab/Week6#Friday 10th August 2007|Fri 10th Aug 2007 (2)]]