Paris/August 30

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Expression test of DapA promotor by differential DAP repression

Plasmids: MiniPrep Products

Plasmids: MiniPrep Products
Number	Name	Description	Products in use	Date
MP15	Sigma Att B rev within pSB2K3			August 20
MP16	Sigma Att P within pSB2K3			August 20

Glycerol Stocks

Digestions

Growth kinetic on w121 strains

In order to precise the growth behavior/kinetics of our strain W121 in smaller DAP concentration, we fit those last ones in function of our last kinetics results (06/08/07)

Kinetic Array :w121 kinetic as a function of DAP and supplemented medium (S0.x)
	1	2	3	4	5	6	7	8	9	10	11	12
A	H₂0	H₂0	H₂0	H₂0	H₂0	H₂0	H₂0	H₂0	H₂0	H₂0	H₂0	H₂0
B	H₂0	LB+0µM DAP	LB+5µM DAP	LB+8µM DAP	LB+10µM DAP	LB+12µM DAP	LB+15µM DAP	LB+17µM DAP	LB+20µM DAP	LB+30µM DAP	LB+40µM DAP	H₂0
C	H₂0	S0.2+0µM DAP	S0.2+5µM DAP	S0.2+6µM DAP	S0.2+7µM DAP	S0.2+8µM DAP	S0.2+9µM DAP	S0.2+10µM DAP	S0.2+15µM DAP	S0.2+17µM DAP	S0.2+20µM DAP	H₂0
D	H₂0	S0.4+0µM DAP	S0.4+2µM DAP	S0.4+3µM DAP	S0.4+4µM DAP	S0.4+5µM DAP	S0.4+8µM DAP	S0.4+10µM DAP	S0.4+12µM DAP	S0.4+15µM DAP	S0.4+20µM DAP	H₂0
E	H₂0	S0.6+0µM DAP	S0.6+1µM DAP	S0.6+2µM DAP	S0.6+3µM DAP	S0.6+4µM DAP	S0.6+5µM DAP	S0.6+6µM DAP	S0.6+7µM DAP	S0.6+8µM DAP	S0.6+10µM DAP	H₂0
F	H₂0	S0.8+0µM DAP	S0.8+0,5µM DAP	S0.8+1µM DAP	S0.8+2µM DAP	S0.8+3µM DAP	S0.8+4µM DAP	S0.8+5µM DAP	S0.8+6µM DAP	S0.8+7µM DAP	S0.8+10µM DAP	H₂0
G	H₂0	H₂0	H₂0	H₂0	H₂0	H₂0	H₂0	H₂0	H₂0	H₂0	H₂0	H₂0
H	H₂0	H₂0	H₂0	H₂0	H₂0	H₂0	H₂0	H₂0	H₂0	H₂0	H₂0	H₂0

The 96 well plate is surrounded by H₂O in order to maintain humidity during the assay. In each slot, 200µL of the growth medium (LB or S0.x) is mixed with 2µL of w121 culture grown ON, and with different amount of DAP (see table). The growth profile is measured over a period of 20H, a DO measurement is acquired each 4min10s.

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 == Glycerol Stocks ==

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