Paris/August 30
From 2007.igem.org
< Paris
Contents |
Expression test of DapA promotor by differential DAP repression
Plasmids: MiniPrep Products
| Plasmids: MiniPrep Products | ||||
|---|---|---|---|---|
| Number | Name | Description | Products in use | Date |
| MP15 | Att B | Lambda Att B rev within pSB2K3 | August 30 | |
| MP16 | Att P | Lambda Att P within pSB2K3 | August 30 | |
Strains (glycerol stock)
| Strains glycerol stock | ||||
|---|---|---|---|---|
| Number | Name | Description | Verified clone | Date |
| S46 | MP15 | Lambda Att B rev within pSB2K3 | August 30 | |
| S47 | MP16 | Lambda Att P within pSB2K3 | August 30 | |
Digestions
we digest:
- MP1.1 as Bw vector with Spe1 & Pst1
- MP15 as Bw insert with Xba1 & Pst1
- MP16 as Bw insert with Xba1 & Pst1
Protocol:
ADN: 20µL
BSA: 0.5µL
NE Buffer 2: 5µL
Enzyme 1: 2µL
Enzyme 2: 2µL
H2O: 20.5µL
