From 2007.igem.org

yesterday -- tomorrow

Growth kinetic on w121 strains

In order to precise the growth behavior/kinetics of our strain W121 in smaller DAP concentration, we fit those last ones in function of our last kinetics results (06/08/07).
But we will use this experiment to better understand the presence of the delay observed in low DAP conentration.

Kinetic Array :w121 kinetic as a function of DAP and supplemented medium (S0.x)
	1	2	3	4	5	6	7	8	9	10	11	12
A	H20	H20	H20	H20	H20	H20	H20	H20	H20	H20	H20	H20
B	H20	LB+0µM DAP	LB+5µM DAP	LB+8µM DAP	LB+10µM DAP	LB+12µM DAP	LB+15µM DAP	LB+17µM DAP	LB+20µM DAP	LB+30µM DAP	LB+40µM DAP	H20
C	H20	S0.2+0µM DAP	S0.2+5µM DAP	S0.2+6µM DAP	S0.2+7µM DAP	S0.2+8µM DAP	S0.2+9µM DAP	S0.2+10µM DAP	S0.2+15µM DAP	S0.2+17µM DAP	S0.2+20µM DAP	H20
D	H20	S0.4+0µM DAP	S0.4+2µM DAP	S0.4+3µM DAP	S0.4+4µM DAP	S0.4+5µM DAP	S0.4+8µM DAP	S0.4+10µM DAP	S0.4+12µM DAP	S0.4+15µM DAP	S0.4+20µM DAP	H20
E	H20	S0.6+0µM DAP	S0.6+1µM DAP	S0.6+2µM DAP	S0.6+3µM DAP	S0.6+4µM DAP	S0.6+5µM DAP	S0.6+6µM DAP	S0.6+7µM DAP	S0.6+8µM DAP	S0.6+10µM DAP	H20
F	H20	S0.8+0µM DAP	S0.8+0,5µM DAP	S0.8+1µM DAP	S0.8+2µM DAP	S0.8+3µM DAP	S0.8+4µM DAP	S0.8+5µM DAP	S0.8+6µM DAP	S0.8+7µM DAP	S0.8+10µM DAP	H20
G	H20	H20	H20	H20	H20	H20	H20	H20	H20	H20	H20	H20
H	H20	H20	H20	H20	H20	H20	H20	H20	H20	H20	H20	H20

The 96 well plate is surrounded by H₂O in order to maintain humidity during the assay. In each slot, 200µL of the growth medium (LB or S0.x) is mixed with 2µL of w121 culture grown ON, and with different amount of DAP (see table). The growth profile is measured over a period of 20H, a DO measurement is acquired each 4min10s.

In order to determine whether the delay observed would come from the growth of a mutant, we'll retake the broth with a 100X dilution. Will this delay appear again and the same way as first observed ?