Glasgow/Wetlab/Week10

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[[User:Christinemerrick|Christine's]] plates from 31/08/07 all grew except for 1B. Overnights were set up from 7 colonies on each of the plates, labelled 1A1→1H7, 49 in total as there were none from plate 1B.
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[[User:Christinemerrick|Christine's]] plates from 31/08/07 all grew except for 1B. Overnights were set up from 7 colonies on each of the plates on Sunday, labelled 1A1→1H7, 49 in total as there were none from plate 1B. [[User:Christinemerrick|Christine]], [[User:Mojs|Maia]] and [[User:MaijaP|Maija]] miniprpped them and digested them as follows:
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{| border="1" cellspacing="0" cellpadding="5" align="center"
 +
|-
 +
!PCR tube
 +
!Colony DNA is taken from
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!Insert
 +
!Enzyme
 +
!Expected sizes
 +
!Result
 +
|-
 +
|1A
 +
|D
 +
|(*a→d*)
 +
|EcoRI
 +
|
 +
|
 +
|-
 +
|1B
 +
|D
 +
|(*a→d*)
 +
|PstI
 +
|
 +
|
 +
|-
 +
|1C
 +
|S
 +
|(*d→g*)
 +
|EcoRI
 +
|
 +
|
 +
|-
 +
|1D
 +
|S
 +
|(*d→g*)
 +
|PstI
 +
|
 +
|
 +
|-
 +
|1E
 +
|S
 +
|(*d→g*)
 +
|PstI
 +
|
 +
|
 +
|-
 +
|1F
 +
|Q
 +
|(*d→g*)
 +
|EcoRI
 +
|
 +
|
 +
|-
 +
|1G
 +
|Q
 +
|(*d→g*)
 +
|PstI
 +
|
 +
|
 +
|-
 +
|1H
 +
|Q
 +
|(*d→g*)
 +
|PstI
 +
|
 +
|
 +
|}
=== Tuesday 4nd September 2007 ===
=== Tuesday 4nd September 2007 ===

Revision as of 14:01, 10 September 2007

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Contents

Week 10

Monday 3st September 2007

  1. Christine's plates from 31/08/07 all grew except for 1B. Overnights were set up from 7 colonies on each of the plates on Sunday, labelled 1A1→1H7, 49 in total as there were none from plate 1B. Christine, Maia and Maija miniprpped them and digested them as follows:
    PCR tube Colony DNA is taken from Insert Enzyme Expected sizes Result
    1A D (*a→d*) EcoRI
    1B D (*a→d*) PstI
    1C S (*d→g*) EcoRI
    1D S (*d→g*) PstI
    1E S (*d→g*) PstI
    1F Q (*d→g*) EcoRI
    1G Q (*d→g*) PstI
    1H Q (*d→g*) PstI

    Tuesday 4nd September 2007

    Wednesday 5rd September 2007

    Thursday 6th September 2007

    Friday 7th September 2007

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    Glasgow's
    Main Page         		Back To
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