Melbourne/Primary Reagents & disposables
From 2007.igem.org
< Melbourne(Difference between revisions)
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*[[Melbourne/IGEM 2007 kit|IGEM 2007 kit]] | *[[Melbourne/IGEM 2007 kit|IGEM 2007 kit]] | ||
*[[Melbourne/Miniprep kit|Miniprep kit]] | *[[Melbourne/Miniprep kit|Miniprep kit]] | ||
| - | *Gel clean | + | *[[Melbourne/Gel clean up kit|Gel clean up kit]] |
*Site directed mutagenesis (stratagene) | *Site directed mutagenesis (stratagene) | ||
**Quickchange multi 200515(x10),200514(x30): 3-5 changes 1 primer each 50% <8Kb | **Quickchange multi 200515(x10),200514(x30): 3-5 changes 1 primer each 50% <8Kb | ||
**Quickchange 200518(x30) 200519(x10): single change 2 primers 90% <8Kb | **Quickchange 200518(x30) 200519(x10): single change 2 primers 90% <8Kb | ||
| - | **QuickchangeII XL 200522(x30) 200521(x10): single change 2 primers 80% >8Kb | + | **[[Melbourne QuickchangeXL|QuickchangeII XL 200522(x30) 200521(x10): single change 2 primers 80% >8Kb]] |
*Primer design program : http://www.stratagene.com/sdmdesigner/default.aspx | *Primer design program : http://www.stratagene.com/sdmdesigner/default.aspx | ||
| - | + | ===Cells=== | |
| + | *[[Melbourne pBluescriptKS|pBluescriptKS+]] | ||
| + | *[[Melbourne DH5a|DH5a super competant cells]] | ||
===Enzymes=== | ===Enzymes=== | ||
*[[Melbourne/primary Restriction enzymes]] | *[[Melbourne/primary Restriction enzymes]] | ||
| - | * | + | *[[Melbourne/primary Ligase|T4 Ligase]] |
| - | + | *[[Melbourne/primary 10X Ligase buffer|T4 Ligase buffer 10X]] | |
| - | + | *[[Melbourne/primary 2X Ligase buffer|T4 Ligase buffer 2X]] | |
| - | + | ||
| - | + | ||
| - | *[[Melbourne/primary Ligase|Ligase]] | + | |
| - | *[[Melbourne/primary | + | |
*[[Melbourne/primary DNA marker|DNA ladder]] | *[[Melbourne/primary DNA marker|DNA ladder]] | ||
| + | *[[Melbourne/primary phosphatase|phospatase]] | ||
| + | *[[Melbourne/primary BSA|BSA]] | ||
| + | *[[Melbourne/primary GoTaq|GoTaq]] | ||
| + | *[[Melbourne/primary vf2|vf2 oligonucleotide]] | ||
| + | *[[Melbourne/primary vR|vR oligonucleotide]] | ||
| + | |||
===Antibiotics=== | ===Antibiotics=== | ||
*[[Melbourne/primary AMP|Ampicilian]] | *[[Melbourne/primary AMP|Ampicilian]] | ||
*[[Melbourne/primary Kan|Kanamycin]] | *[[Melbourne/primary Kan|Kanamycin]] | ||
| + | *[[Melbourne/primary Chloroamphenicol|Chloroamphenicol]] | ||
| + | *[[Melbourne/primary Gen|Gentimycin]] | ||
| + | |||
| + | |||
===Chemicals=== | ===Chemicals=== | ||
*[[Melbourne/primary agar|Agar]] | *[[Melbourne/primary agar|Agar]] | ||
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*[[Melbourne/primary Nacl|NaCl]] | *[[Melbourne/primary Nacl|NaCl]] | ||
*[[Melbourne/primary Tris|TRIS]] | *[[Melbourne/primary Tris|TRIS]] | ||
| + | *[[Melbourne/primary IPTG|IPTG]] | ||
| + | *[[Melbourne/primary Xgal|Xgal]] | ||
| + | *[[Melbourne/primary Bacto-tryptone|Bacto-tryptone]] | ||
| + | *[[Melbourne/primary Yeast Extract|Yeast Extract]] | ||
| + | *[[Melbourne/primary NaOH|NaOH]] | ||
| + | *[[Melbourne/primary NZ amine|NZ amine]] | ||
| + | *[[Melbourne/primary Bacto-yeast Extract|Bacto-yeast Extract]] | ||
| + | *[[Melbourne/primary Ethanol|Ethanol]] | ||
===Other=== | ===Other=== | ||
| + | *[[Melbourne/primary DNA Loading dye|DNA Loading Dye]] | ||
*[[Melbourne/primary ice|ice]] | *[[Melbourne/primary ice|ice]] | ||
*[[Melbourne/primary milliq|milliQ water]] | *[[Melbourne/primary milliq|milliQ water]] | ||
*[[Melbourne/primary distilled|Distilled water]] | *[[Melbourne/primary distilled|Distilled water]] | ||
| + | *[[Melbourne/primary Liquid N2|Liquid Nitrogen]] | ||
| + | *[[Melbourne/primary Solid CO2|Dry Ice]] | ||
| + | |||
==Disposables== | ==Disposables== | ||
*[[Melbourne/primary Parrafilm|Parrafilm]] | *[[Melbourne/primary Parrafilm|Parrafilm]] | ||
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===TUBES:=== | ===TUBES:=== | ||
*[[Melbourne/primary falcon|Falcon: 10ml, 50ml]] | *[[Melbourne/primary falcon|Falcon: 10ml, 50ml]] | ||
| - | *microcentrifuge: 1.7ml | + | *[[Melbourne/1.7ml microcentrifuge|microcentrifuge: 1.7ml]] |
| - | * | + | *[[Melbourne/0.5ml microcentrifuge|microcentrifuge: 0.6ml]] |
| + | *[[Melbourne/Screw Top tubes|Screw Top tubes]] | ||
Latest revision as of 11:48, 8 October 2007
<Back to team home page> <Template>
Contents |
Primary Reagents
Kits
- IGEM 2007 kit
- Miniprep kit
- Gel clean up kit
- Site directed mutagenesis (stratagene)
- Quickchange multi 200515(x10),200514(x30): 3-5 changes 1 primer each 50% <8Kb
- Quickchange 200518(x30) 200519(x10): single change 2 primers 90% <8Kb
- QuickchangeII XL 200522(x30) 200521(x10): single change 2 primers 80% >8Kb
- Primer design program : http://www.stratagene.com/sdmdesigner/default.aspx
Cells
Enzymes
- Melbourne/primary Restriction enzymes
- T4 Ligase
- T4 Ligase buffer 10X
- T4 Ligase buffer 2X
- DNA ladder
- phospatase
- BSA
- GoTaq
- vf2 oligonucleotide
- vR oligonucleotide
Antibiotics
Chemicals
- Agar
- Agarose
- Ethidium Bromide
- EDTA
- Glycerol
- Loading dye for DNA gel
- NaCl
- TRIS
- IPTG
- Xgal
- Bacto-tryptone
- Yeast Extract
- NaOH
- NZ amine
- Bacto-yeast Extract
- Ethanol
Other
Disposables
- Parrafilm
- Tips (10, 200, 1000 ul)
- Gloves (Large , Med, Small)
- Scalpels
- Tube racks
- Freezer boxes
