Paris/Oligos

From 2007.igem.org

< Paris(Difference between revisions)
 
(8 intermediate revisions not shown)
Line 17: Line 17:
| style="background: #ccffcc;" | O2
| style="background: #ccffcc;" | O2
| FtsZ-R
| FtsZ-R
-
| TTTCTGCAGCGGCCGCTACTAGTATTTTTAATCAGCTTGCTTACGCAGGAAT
+
| TTTCTGCAGCGGCCGCTACTAGTATTTTTAATCAGCTTGCTTACGCA GGAAT
| 49
| 49
|  
|  
Line 23: Line 23:
| style="background: #ccffcc;" | O3
| style="background: #ccffcc;" | O3
| Lox71-FtsA-F
| Lox71-FtsA-F
-
| TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACG AAGTTATTAATAATCAGGCACAGGCAGAACAACAATG
+
| TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATT ATACGAAGTTATTAATAATCAGGCACAGGCAGAACAACAATG
| 89
| 89
|  
|  
Line 98: Line 98:
| style="background: #ccffcc;" | O14
| style="background: #ccffcc;" | O14
| Lox66-F       
| Lox66-F       
-
| AATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACGAACGGTATAC TAGTAGCGGCCGCTGCA
+
| AATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACGAAC GGTATACTAGTAGCGGCCGCTGCA
|  
|  
|
|
Line 105: Line 105:
| style="background: #ccffcc;" | O15
| style="background: #ccffcc;" | O15
| Lox66-R
| Lox66-R
-
| GCGGCCGCTACTAGTATACCGTTCGTATAATGTATGCTATACCAAGTTATCTCTAG AAGCGGCCGCG
+
| GCGGCCGCTACTAGTATACCGTTCGTATAATGTATGCTATACCAAGTTATC TCTAGAAGCGGCCGCG
|  
|  
|
|
Line 112: Line 112:
| style="background: #ccffcc;" | O16
| style="background: #ccffcc;" | O16
| Lox71-F
| Lox71-F
-
| AATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAGTTATTACT AGTAGCGGCCGCTGCA
+
| AATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAG TTATTACTAGTAGCGGCCGCTGCA
|  
|  
|
|
Line 119: Line 119:
| style="background: #ccffcc;" | O17
| style="background: #ccffcc;" | O17
| Lox71-R
| Lox71-R
-
| GCGGCCGCTACTAGTAATAACTTCGTATAATGTATCGTATACGAACGGTACTCTAG AAGCGGCCGCG
+
| GCGGCCGCTACTAGTAATAACTTCGTATAATGTATCGTATACGAACGGTAC TCTAGAAGCGGCCGCG
|  
|  
|
|
Line 140: Line 140:
| style="background: #ccffcc;" | O20
| style="background: #ccffcc;" | O20
| RBS-DapAColi-F
| RBS-DapAColi-F
-
| TTTGAATTCGCGGCCGCTTCTAGAGATTAAAGAGGAGAAATACTAGATGTT CACGGGAAGTATTGTCGCGA
+
| TTTGAATTCGCGGCCGCTTCTAGAGATTAAAGAGGAGAAATACTAGATGT TCACGGGAAGTATTGTCGCGA
| 71
| 71
| Biobrick prefix + Strong RBS BBa_B0030 + 25 bp homology to DapA (Coli) from ATG
| Biobrick prefix + Strong RBS BBa_B0030 + 25 bp homology to DapA (Coli) from ATG
Line 203: Line 203:
| style="background: #ccffcc;" | O29
| style="background: #ccffcc;" | O29
| ForDGAT_BB1
| ForDGAT_BB1
-
| AAAGAATTCGCGGCCGCTTCTAGAGATTAAAGAGGAGAAAATCCACGCTATGCGCCCATTAC
+
| AAAGAATTCGCGGCCGCTTCTAGAGATTAAAGAGGAGAAAATCCACGCTATG CGCCCATTAC
| 62
| 62
| Tm = 60/83°C
| Tm = 60/83°C
Line 289: Line 289:
| style="background: #ccffcc;" | O43
| style="background: #ccffcc;" | O43
| Bfr-FtsK-F
| Bfr-FtsK-F
-
| TTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAATGCCACCTGACGTCTAAGAA
+
| TTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAAT GCCACCTGACGTCTAAGAA
| 70  
| 70  
|  
|  
Line 295: Line 295:
| style="background: #ccffcc;" | O44
| style="background: #ccffcc;" | O44
| Bfr-FtsK-R
| Bfr-FtsK-R
-
| CCAGGTTACCCCAGTACTGTTGCTGTATGGATTAACAGGAGTGTAATCAAATTACCGCCTTTGAGTGAGC
+
| CCAGGTTACCCCAGTACTGTTGCTGTATGGATTAACAGGAGTGTAATCAAA TTACCGCCTTTGAGTGAGC
| 70  
| 70  
|
|
Line 301: Line 301:
| style="background: #ccffcc;" | O45
| style="background: #ccffcc;" | O45
| Aft-FtsK-F
| Aft-FtsK-F
-
| AGGGGCACAACGGTAATCGTGAAGTGCTGGCCCCACCGCCGTTTGACTAATGCCACCTGACGTCTAAGAA
+
| AGGGGCACAACGGTAATCGTGAAGTGCTGGCCCCACCGCCGTTTGACTAAT GCCACCTGACGTCTAAGAA
| 70  
| 70  
|
|
Line 307: Line 307:
| style="background: #ccffcc;" | O46
| style="background: #ccffcc;" | O46
| Aft-FtsK-R
| Aft-FtsK-R
-
| GAAAATACTGAATTTTCAGTTGATAGAGTGCCGGATGCGACGCTACCGCGATTACCGCCTTTGAGTGAGC
+
| GAAAATACTGAATTTTCAGTTGATAGAGTGCCGGATGCGACGCTACCGCGA TTACCGCCTTTGAGTGAGC
| 70  
| 70  
 +
|
 +
|- style="background: #cccccc;"
 +
| style="background: #ccffcc;" | O47
 +
| PacI-pTet-F
 +
| TTATTAATTAAGAATTCGCGGCCGCATCTA
 +
| 30
 +
|
 +
|- style="background: #cccccc;"
 +
| style="background: #ccffcc;" | O48
 +
| XmaI-mrfpBB-R
 +
| GCATCCCGGGCAGCGGCCGCTTCTAGTATA
 +
| 30
 +
|
 +
|- style="background: #cccccc;"
 +
| style="background: #ccffcc;" | O49
 +
| PacI-pTet-F - oligold
 +
| GCTTATTAATTAAGAATTCGCGGCCGCATCTAG
 +
| 33
 +
|
 +
|- style="background: #cccccc;"
 +
| style="background: #ccffcc;" | O50
 +
| XmaI-mrfpBB-R - oligold
 +
| GGCATCCCGGGCAGCGGCCGCTTCTAGTATA
 +
| 31
 +
|
 +
|- style="background: #cccccc;"
 +
| style="background: #ccffcc;" | O51
 +
| Prefix-frt2-R
 +
| TTTCTGGAATTCGCGGCCGCATCTAGAATGGTTCGCTTCAGATCGTT
 +
| 47
 +
|
 +
|- style="background: #cccccc;"
 +
| style="background: #ccffcc;" | O52
 +
| SufixBB-frt1-F
 +
| AGGAAGCCTGCAGCGGCCGCTACTAGTA - TCTACAAACTCTGCTAGTTT - GTTTCGAAGTTCCTATACTT   
 +
final version with 5bp homology in frt1 & a mismatch at 14th bp (A>G) inabelling the SpeI site: GAAGCCTGCAGCGGCCGCTACTAGTAAACTCTGCTAGTTTCGAAGT
 +
Superfinal version with 12bp homology to each side from the mismatch (13th bp - A>G) : AGGAAGCCTGCAGCGGCCGCTACTAGTA - TCTACAAACTCTGCTAGTTTCGAAG
 +
| 48
 +
|
 +
|- style="background: #cccccc;"
 +
| style="background: #ccffcc;" | O53
 +
| SufixBB-T1T2frt1-F
 +
| ATTAAGCCTGCAGCGGCCGCTACTAGTAAATGGATCCCCCGGGGGCTG
 +
| 48
|
|
|}
|}

Latest revision as of 23:46, 14 October 2007

Table of Oligos
Number Name Sequence Length Comments
O1 Lox71-FtsZ-F TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATT ATACGAAGTTATTAATAAACAGGCACAAATCGGAGAGAAACT 89
O2 FtsZ-R TTTCTGCAGCGGCCGCTACTAGTATTTTTAATCAGCTTGCTTACGCA GGAAT 49
O3 Lox71-FtsA-F TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATT ATACGAAGTTATTAATAATCAGGCACAGGCAGAACAACAATG 89
O4 DEcoR1-FtsZ-R CGGTATTTACCGCGAAGAACTCAACACCTTCAATGCGCTC 40
O5 DEcoR1-FtsZ-F TGCGCGAGCGCATTGAAGGTGTTGAGTTCTTCGCGGTAAA 40
O6 Lox66-DapAColi-F TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACG AACGGTAGTTTGCACAGAGGATGGCCCATGTT 84
O7 DapAColi-R TTTCTGCAGCGGCCGCTACTAGTATTATTACAGCAAACCGGCATGCTTAAGC 52
O8 Lox66-DapASubtilis-F TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACG AACGGTAATCATGATGAGGTGAATAAAATGAA 84
O9 DapASubtilis-R TTTCTGCAGCGGCCGCTACTAGTATTATTACAGTTCGCTGATCGTGCTGCTT 52
O10 Lox71-KmR-F TTTCTCTAGAAGCGGCCGCGAATTCTACCGTTCGTATACGATACATTATACG AAGTTATCTGATCCTTCAACTCAGCAAAAGTTC 85
O11 Lox66-KmR-R TTTTACTAGTAGCGGCCGCTGCAGTACCGTTCGTATAATGTATGCTATACCA AGTTATTTATTAGAAAAACTCATCGAGCATCAAA 86
O12 DPst1-DGAT-F AGTGCTGTCGTATATGGCCCAGCAGGACTCAACATAATTT 40
O13 DPst1-DGAT-R AAATTATGTTGAGTCCTGCTGGGCCATATACGACAGCACT 40


O14 Lox66-F AATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACGAAC GGTATACTAGTAGCGGCCGCTGCA
O15 Lox66-R GCGGCCGCTACTAGTATACCGTTCGTATAATGTATGCTATACCAAGTTATC TCTAGAAGCGGCCGCG
O16 Lox71-F AATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAG TTATTACTAGTAGCGGCCGCTGCA
O17 Lox71-R GCGGCCGCTACTAGTAATAACTTCGTATAATGTATCGTATACGAACGGTAC TCTAGAAGCGGCCGCG
O18 VF2 tgccacctgacgtctaagaa 20 Forward Primer to Biobrick vectors (VF2) BBa_G00100
O19 VR attaccgcctttgagtgagc 20 Reverse Primer to Biobrick Vectors (VR) BBa_G00101
O20 RBS-DapAColi-F TTTGAATTCGCGGCCGCTTCTAGAGATTAAAGAGGAGAAATACTAGATGT TCACGGGAAGTATTGTCGCGA 71 Biobrick prefix + Strong RBS BBa_B0030 + 25 bp homology to DapA (Coli) from ATG
O21 RBS-DapASubtilis-F TTTGAATTCGCGGCCGCTTCTAGAGATTAAAGAGGAGAAATACTAGATGAA TTTCGGAAATGTGTCTACAG 71 Biobrick prefix + Strong RBS BBa_B0030 + 25 bp homology to DapA (Subtilis) from ATG
O22 SEQFtsA-1F GTCGTCCATACCGCGAAATC 20
O23 SEQFtsA-2F CGTGGAAGTGCCGAGCGTAG 20
O24 SEQFtsZ-1F GGTATCGCTGAACTGATTAC 20
O25 SEQDapASub-1F TTAAAGCAATTGCGGCAGAG 20
O26 SEQDapAColi-1F CAATCGTCCGTCGCAAGAAG 20
O27 ForDGAT1 ATCCACGCTATGCGCCCATTAC 22 Tm = 60°C
O28 RevDGAT1 GGGCTAATTTAGCCCTTTAGTT 22 Tm = 50°C
O29 ForDGAT_BB1 AAAGAATTCGCGGCCGCTTCTAGAGATTAAAGAGGAGAAAATCCACGCTATG CGCCCATTAC 62 Tm = 60/83°C
O30 RevDGAT_BB1 CTGCAGCGGCCGCTACTAGTAGGGCTAATTTAGCCCTTTAGTT 43 Tm = 50/74°C
O31 DapAp-F aattcgcggccgcttctagagAACAATCAGAACGGTTCTGTCTGCTTGCTTTT AATGCCATACCAAACGTACCATTGAGACACTTGTTTGCACAGAGGATGGta 104
O32 DapAp-R ctagtaCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCA TTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTctctagaagcggccgcg 104
O33 dDapA-F CATACCAAACGTACCATTGAGACACTTGTTTGCACAGAGGAT GGCCCATGATTCCGGGGATCCGTCGACC 70 more here
O34 dDapA-R AAGCCATCAAATCTCCCTAAACTTTACAGCAAACCGGCATGC TTAAGCGCTGTAGGCTGGAGCTGCTTCG 70 more here
O35 dFtsZ-F GACGATGATTACGGCCTCAGGCGACAGGCACAAATCGGAGAGA AACTATGATTCCGGGGATCCGTCGACC 70 more here
O36 dFtsZ-R GAAACCCAAATTCCAGTCAATTCTTAATCAGCTTGCTTACGCA GGAATGCTGTAGGCTGGAGCTGCTTCG 70 more here
O37 dDapAverif-F ACAATCAGAACGGTTCTGTCTG 22
O38 dDapAverif-R ATAGCGTGAGTCAGAACTACAG 22
O39 dFtsZverif-R TCGCCAAATTACCTATCCAACC 22
O40 SEQ-mRFP-R CGGACGACCTTCACCTTCAC
O41 M13(-20)-F GTAAAACGACGGCCAG
O42 M13-R CAGGAAACAGCTTATGAC
O43 Bfr-FtsK-F TTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAAT GCCACCTGACGTCTAAGAA 70
O44 Bfr-FtsK-R CCAGGTTACCCCAGTACTGTTGCTGTATGGATTAACAGGAGTGTAATCAAA TTACCGCCTTTGAGTGAGC 70
O45 Aft-FtsK-F AGGGGCACAACGGTAATCGTGAAGTGCTGGCCCCACCGCCGTTTGACTAAT GCCACCTGACGTCTAAGAA 70
O46 Aft-FtsK-R GAAAATACTGAATTTTCAGTTGATAGAGTGCCGGATGCGACGCTACCGCGA TTACCGCCTTTGAGTGAGC 70
O47 PacI-pTet-F TTATTAATTAAGAATTCGCGGCCGCATCTA 30
O48 XmaI-mrfpBB-R GCATCCCGGGCAGCGGCCGCTTCTAGTATA 30
O49 PacI-pTet-F - oligold GCTTATTAATTAAGAATTCGCGGCCGCATCTAG 33
O50 XmaI-mrfpBB-R - oligold GGCATCCCGGGCAGCGGCCGCTTCTAGTATA 31
O51 Prefix-frt2-R TTTCTGGAATTCGCGGCCGCATCTAGAATGGTTCGCTTCAGATCGTT 47
O52 SufixBB-frt1-F AGGAAGCCTGCAGCGGCCGCTACTAGTA - TCTACAAACTCTGCTAGTTT - GTTTCGAAGTTCCTATACTT

final version with 5bp homology in frt1 & a mismatch at 14th bp (A>G) inabelling the SpeI site: GAAGCCTGCAGCGGCCGCTACTAGTAAACTCTGCTAGTTTCGAAGT Superfinal version with 12bp homology to each side from the mismatch (13th bp - A>G) : AGGAAGCCTGCAGCGGCCGCTACTAGTA - TCTACAAACTCTGCTAGTTTCGAAG

48
O53 SufixBB-T1T2frt1-F ATTAAGCCTGCAGCGGCCGCTACTAGTAAATGGATCCCCCGGGGGCTG 48
Retrieved from "http://2007.igem.org/wiki/index.php/Paris/Oligos"