Paris/Oligos
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| style="background: #ccffcc;" | O47 | | style="background: #ccffcc;" | O47 | ||
| PacI-pTet-F | | PacI-pTet-F | ||
| - | | | + | | TTATTAATTAAGAATTCGCGGCCGCATCTA |
| - | | | + | | 30 |
| | | | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" | O48 | | style="background: #ccffcc;" | O48 | ||
| XmaI-mrfpBB-R | | XmaI-mrfpBB-R | ||
| + | | GCATCCCGGGCAGCGGCCGCTTCTAGTATA | ||
| + | | 30 | ||
| + | | | ||
| + | |- style="background: #cccccc;" | ||
| + | | style="background: #ccffcc;" | O49 | ||
| + | | PacI-pTet-F - oligold | ||
| + | | GCTTATTAATTAAGAATTCGCGGCCGCATCTAG | ||
| + | | 33 | ||
| + | | | ||
| + | |- style="background: #cccccc;" | ||
| + | | style="background: #ccffcc;" | O50 | ||
| + | | XmaI-mrfpBB-R - oligold | ||
| GGCATCCCGGGCAGCGGCCGCTTCTAGTATA | | GGCATCCCGGGCAGCGGCCGCTTCTAGTATA | ||
| 31 | | 31 | ||
| | | | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| - | | style="background: #ccffcc;" | | + | | style="background: #ccffcc;" | O51 |
| Prefix-frt2-R | | Prefix-frt2-R | ||
| TTTCTGGAATTCGCGGCCGCATCTAGAATGGTTCGCTTCAGATCGTT | | TTTCTGGAATTCGCGGCCGCATCTAGAATGGTTCGCTTCAGATCGTT | ||
| Line 329: | Line 341: | ||
| | | | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| - | | style="background: #ccffcc;" | | + | | style="background: #ccffcc;" | O52 |
| SufixBB-frt1-F | | SufixBB-frt1-F | ||
| - | | | + | | AGGAAGCCTGCAGCGGCCGCTACTAGTA - TCTACAAACTCTGCTAGTTT - GTTTCGAAGTTCCTATACTT |
| + | final version with 5bp homology in frt1 & a mismatch at 14th bp (A>G) inabelling the SpeI site: GAAGCCTGCAGCGGCCGCTACTAGTAAACTCTGCTAGTTTCGAAGT | ||
| + | Superfinal version with 12bp homology to each side from the mismatch (13th bp - A>G) : AGGAAGCCTGCAGCGGCCGCTACTAGTA - TCTACAAACTCTGCTAGTTTCGAAG | ||
| 48 | | 48 | ||
| | | | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| - | | style="background: #ccffcc;" | | + | | style="background: #ccffcc;" | O53 |
| SufixBB-T1T2frt1-F | | SufixBB-T1T2frt1-F | ||
| ATTAAGCCTGCAGCGGCCGCTACTAGTAAATGGATCCCCCGGGGGCTG | | ATTAAGCCTGCAGCGGCCGCTACTAGTAAATGGATCCCCCGGGGGCTG | ||
Latest revision as of 23:46, 14 October 2007
| Table of Oligos | ||||
|---|---|---|---|---|
| Number | Name | Sequence | Length | Comments |
| O1 | Lox71-FtsZ-F | TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATT ATACGAAGTTATTAATAAACAGGCACAAATCGGAGAGAAACT | 89 | |
| O2 | FtsZ-R | TTTCTGCAGCGGCCGCTACTAGTATTTTTAATCAGCTTGCTTACGCA GGAAT | 49 | |
| O3 | Lox71-FtsA-F | TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATT ATACGAAGTTATTAATAATCAGGCACAGGCAGAACAACAATG | 89 | |
| O4 | DEcoR1-FtsZ-R | CGGTATTTACCGCGAAGAACTCAACACCTTCAATGCGCTC | 40 | |
| O5 | DEcoR1-FtsZ-F | TGCGCGAGCGCATTGAAGGTGTTGAGTTCTTCGCGGTAAA | 40 | |
| O6 | Lox66-DapAColi-F | TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACG AACGGTAGTTTGCACAGAGGATGGCCCATGTT | 84 | |
| O7 | DapAColi-R | TTTCTGCAGCGGCCGCTACTAGTATTATTACAGCAAACCGGCATGCTTAAGC | 52 | |
| O8 | Lox66-DapASubtilis-F | TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACG AACGGTAATCATGATGAGGTGAATAAAATGAA | 84 | |
| O9 | DapASubtilis-R | TTTCTGCAGCGGCCGCTACTAGTATTATTACAGTTCGCTGATCGTGCTGCTT | 52 | |
| O10 | Lox71-KmR-F | TTTCTCTAGAAGCGGCCGCGAATTCTACCGTTCGTATACGATACATTATACG AAGTTATCTGATCCTTCAACTCAGCAAAAGTTC | 85 | |
| O11 | Lox66-KmR-R | TTTTACTAGTAGCGGCCGCTGCAGTACCGTTCGTATAATGTATGCTATACCA AGTTATTTATTAGAAAAACTCATCGAGCATCAAA | 86 | |
| O12 | DPst1-DGAT-F | AGTGCTGTCGTATATGGCCCAGCAGGACTCAACATAATTT | 40 | |
| O13 | DPst1-DGAT-R | AAATTATGTTGAGTCCTGCTGGGCCATATACGACAGCACT | 40 |
|
| O14 | Lox66-F | AATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACGAAC GGTATACTAGTAGCGGCCGCTGCA | ||
| O15 | Lox66-R | GCGGCCGCTACTAGTATACCGTTCGTATAATGTATGCTATACCAAGTTATC TCTAGAAGCGGCCGCG | ||
| O16 | Lox71-F | AATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAG TTATTACTAGTAGCGGCCGCTGCA | ||
| O17 | Lox71-R | GCGGCCGCTACTAGTAATAACTTCGTATAATGTATCGTATACGAACGGTAC TCTAGAAGCGGCCGCG | ||
| O18 | VF2 | tgccacctgacgtctaagaa | 20 | Forward Primer to Biobrick vectors (VF2) BBa_G00100 |
| O19 | VR | attaccgcctttgagtgagc | 20 | Reverse Primer to Biobrick Vectors (VR) BBa_G00101 |
| O20 | RBS-DapAColi-F | TTTGAATTCGCGGCCGCTTCTAGAGATTAAAGAGGAGAAATACTAGATGT TCACGGGAAGTATTGTCGCGA | 71 | Biobrick prefix + Strong RBS BBa_B0030 + 25 bp homology to DapA (Coli) from ATG |
| O21 | RBS-DapASubtilis-F | TTTGAATTCGCGGCCGCTTCTAGAGATTAAAGAGGAGAAATACTAGATGAA TTTCGGAAATGTGTCTACAG | 71 | Biobrick prefix + Strong RBS BBa_B0030 + 25 bp homology to DapA (Subtilis) from ATG |
| O22 | SEQFtsA-1F | GTCGTCCATACCGCGAAATC | 20 | |
| O23 | SEQFtsA-2F | CGTGGAAGTGCCGAGCGTAG | 20 | |
| O24 | SEQFtsZ-1F | GGTATCGCTGAACTGATTAC | 20 | |
| O25 | SEQDapASub-1F | TTAAAGCAATTGCGGCAGAG | 20 | |
| O26 | SEQDapAColi-1F | CAATCGTCCGTCGCAAGAAG | 20 | |
| O27 | ForDGAT1 | ATCCACGCTATGCGCCCATTAC | 22 | Tm = 60°C |
| O28 | RevDGAT1 | GGGCTAATTTAGCCCTTTAGTT | 22 | Tm = 50°C |
| O29 | ForDGAT_BB1 | AAAGAATTCGCGGCCGCTTCTAGAGATTAAAGAGGAGAAAATCCACGCTATG CGCCCATTAC | 62 | Tm = 60/83°C |
| O30 | RevDGAT_BB1 | CTGCAGCGGCCGCTACTAGTAGGGCTAATTTAGCCCTTTAGTT | 43 | Tm = 50/74°C |
| O31 | DapAp-F | aattcgcggccgcttctagagAACAATCAGAACGGTTCTGTCTGCTTGCTTTT AATGCCATACCAAACGTACCATTGAGACACTTGTTTGCACAGAGGATGGta | 104 | |
| O32 | DapAp-R | ctagtaCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCA TTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTctctagaagcggccgcg | 104 | |
| O33 | dDapA-F | CATACCAAACGTACCATTGAGACACTTGTTTGCACAGAGGAT GGCCCATGATTCCGGGGATCCGTCGACC | 70 | more here |
| O34 | dDapA-R | AAGCCATCAAATCTCCCTAAACTTTACAGCAAACCGGCATGC TTAAGCGCTGTAGGCTGGAGCTGCTTCG | 70 | more here |
| O35 | dFtsZ-F | GACGATGATTACGGCCTCAGGCGACAGGCACAAATCGGAGAGA AACTATGATTCCGGGGATCCGTCGACC | 70 | more here |
| O36 | dFtsZ-R | GAAACCCAAATTCCAGTCAATTCTTAATCAGCTTGCTTACGCA GGAATGCTGTAGGCTGGAGCTGCTTCG | 70 | more here |
| O37 | dDapAverif-F | ACAATCAGAACGGTTCTGTCTG | 22 | |
| O38 | dDapAverif-R | ATAGCGTGAGTCAGAACTACAG | 22 | |
| O39 | dFtsZverif-R | TCGCCAAATTACCTATCCAACC | 22 | |
| O40 | SEQ-mRFP-R | CGGACGACCTTCACCTTCAC | ||
| O41 | M13(-20)-F | GTAAAACGACGGCCAG | ||
| O42 | M13-R | CAGGAAACAGCTTATGAC | ||
| O43 | Bfr-FtsK-F | TTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAAT GCCACCTGACGTCTAAGAA | 70 | |
| O44 | Bfr-FtsK-R | CCAGGTTACCCCAGTACTGTTGCTGTATGGATTAACAGGAGTGTAATCAAA TTACCGCCTTTGAGTGAGC | 70 | |
| O45 | Aft-FtsK-F | AGGGGCACAACGGTAATCGTGAAGTGCTGGCCCCACCGCCGTTTGACTAAT GCCACCTGACGTCTAAGAA | 70 | |
| O46 | Aft-FtsK-R | GAAAATACTGAATTTTCAGTTGATAGAGTGCCGGATGCGACGCTACCGCGA TTACCGCCTTTGAGTGAGC | 70 | |
| O47 | PacI-pTet-F | TTATTAATTAAGAATTCGCGGCCGCATCTA | 30 | |
| O48 | XmaI-mrfpBB-R | GCATCCCGGGCAGCGGCCGCTTCTAGTATA | 30 | |
| O49 | PacI-pTet-F - oligold | GCTTATTAATTAAGAATTCGCGGCCGCATCTAG | 33 | |
| O50 | XmaI-mrfpBB-R - oligold | GGCATCCCGGGCAGCGGCCGCTTCTAGTATA | 31 | |
| O51 | Prefix-frt2-R | TTTCTGGAATTCGCGGCCGCATCTAGAATGGTTCGCTTCAGATCGTT | 47 | |
| O52 | SufixBB-frt1-F | AGGAAGCCTGCAGCGGCCGCTACTAGTA - TCTACAAACTCTGCTAGTTT - GTTTCGAAGTTCCTATACTT
final version with 5bp homology in frt1 & a mismatch at 14th bp (A>G) inabelling the SpeI site: GAAGCCTGCAGCGGCCGCTACTAGTAAACTCTGCTAGTTTCGAAGT Superfinal version with 12bp homology to each side from the mismatch (13th bp - A>G) : AGGAAGCCTGCAGCGGCCGCTACTAGTA - TCTACAAACTCTGCTAGTTTCGAAG | 48 | |
| O53 | SufixBB-T1T2frt1-F | ATTAAGCCTGCAGCGGCCGCTACTAGTAAATGGATCCCCCGGGGGCTG | 48 | |
