Paris/October 17

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Unfortunatly, D82 digestion reaction gave an unexpected result with a band at 500bp

Latest revision as of 03:40, 21 October 2007

yesterday -- tomorrow


Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 Size Description
D94 PacI[ptet>>lox71-gfpT-lox66-mrfp]SmaI P15 PacI XmaI ptet>>lox71-gfpT-lox66-mrfp P15 PCR product digested by PacI-SmaI for cloning into pY-2C-IntC
D95 PacI[pY-2C-IntC]SmaI pY-2C-IntC plasmid PacI XmaI pY-2C-IntC digested for ptet>>lox71-gfpT-lox66-mrfp cloning
D82 frt1-CmR-frt2 FI P16 EcoRI SpeI frt1-CmR-frt2 PCR product digested for use as a FI
D83 frt1-CmR-frt2 BI P16 XbaI PstI frt1-CmR-frt2 PCR product digested for use as a BI
D84 frt1-CmR-frt2 BBi P16 EcoRI PstI frt1-CmR-frt2 PCR product digested for cloning as a BB


Unfortunatly, D82 digestion reaction gave an unexpected result with a band at 500bp

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