Glasgow/Wetlab/Orders
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Revision as of 16:53, 22 October 2007
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Emma's primers
| Name | Sequence | Tm |
|---|---|---|
| * PhzUp | 5´ TAAGGATCCGGTAGTTCCAAGCCCCAGAAAC 3´ | 65.6 |
| * PhzLow | 5´ CACATTTGATCTAGATGGGTCACGGCTATTCAG 3´ | 64.8 |
| *Bbp_PhzUp | 5´ gaattcgcggccgcttctagaTAAGGATCCGGTAGTTCCAAGCCCCAGAAAC 3´ | 63.6 |
| *Bbs_PhzLow | 5´ ctgcagcggccgctactagtaCACATTTGATCTAGATGGGTCACGGCTATTCAG 3´ | 64.2 |
| Bbp_Gene | 5´ GAATTCGCGGCCGCTTCTAG 3´ | 65.7 |
| Bbs | 5´ CTGCAGCGGCCGCTACTAGTA 3´ | 65.3 |
| *Methyl_1 | 5´ TTTTTGAATTCTTTCGGACGCAGGAAAAG 3´ | 66.3 |
| *Methyl_2 | 5´ TTTTTGGATCCGTTGAAAGTTCCGATTCA 3´ | 66.2 |
| *Bbp_Methyl_1 | 5´ gaattcgcggccgcttctagaTTTTTGAATTCTTTCGGACGCAGGAAAAG 3´ | 63.9 |
| *Bbs_Methyl_2 | 5´ ctgcagcggccgctactagtaTTTTTGGATCCGTTGAAAGTTCCGATTCA 3´ | 66.9 |
| PhzM_for_1 | 5´ atgaataattcgaatcttgctgct 3´ | 65.4 |
| PhzM_rev_1 | 5´ tcaggccctggcagcgac 3´ | 60.3 |
| Bbp_PhzM_for_1 | 5´ gaattcgcggccgcttctagatgaataattcgaatcttgctgct 3´ | 64.5 |
| Bbs_PhzM_rev_1 | 5´ ctgcagcggccgctactagtatcaggccctggcagcgac 3´ | 60.3 |
| *Oxy_1 | 5´TCGAACACTCTAGAAAAGGAAGCACC 3´ | 60.2 |
| *Oxy_2 | 5´ TTTTTTGCATGCTAGCGTGGCCGTTCC 3´ | 59.2 |
| *Bbp_Oxy_1 | 5´ gaattcgcggccgcttctagaTCGAACACTCTAGAAAAGGAAGCACC 3´ | 65.1 |
| *Bbs_Oxy_2 | 5´ ctgcagcggccgctactagtaTTTTTTGCATGCTAGCGTGGCCGTTCC 3´ | 66.9 |
| PhzS_for_1 | 5´ atgagcgaacccatcgatat 3´ | 71.2 |
| PhzS_rev_1 | 5´ ctagcgtggccgttccacctg 3´ | 60.2 |
| Bbp_PhzS_for_1 | 5´ gaattcgcggccgcttctagatgagcgaacccatcgatat 3´ | 70.9 |
| Bbs_PhzS_rev_1 | 5´ ctgcagcggccgctactagtactagcgtggccgttccacctg 3´ | 63.3 |
| PhzH_up | 5´ CGCACGGATCCTTTCAGAATGTTC 3´ | 69.9 |
| PhzH_low | 5´ GCCACGCCAAGCTTCACGCTCA 3´ | 70.6 |
| Bbp_PhzUp | 5´ gaattcgcggccgcttctagaTAAGGATCCGGTAGTTCCAAGCCCCAGAAAC 3´ | 63.3 |
| Bbs_PhzLow | 5´ ctgcagcggccgctactagtaCACATTTGATCTAGATGGGTCACGGCTATTCAG 3´ | 57.7 |
Designed primers
| Name | Sequence | Tm |
|---|---|---|
| DmpR_SDM_PstI_1_for | 5´ CTGGAGCAACAGCATGCGCTGTTC 3´ | 65.6 |
| DmpR_SDM_PstI_1_rev | 5´ GCTCCAGTTTTCAGCGATGGCC 3´ | 64.8 |
| DmpR_SDM_PstI_2_for | 5´ CTGGAGGAAGTGCTCAACCAGCAG 3´ | 66.3 |
| DmpR_SDM_PstI_2_rev | 5´ CCTCCAGCGCTTCCACCAG 3´ | 66.2 |
| DmpR_Start_Prefix_1_for | 5´ GAATTCGCGGCCGCTTCTAGATGCCGATCAAGTACAAGCCTG 3´ | 60.2 |
| DmpR_End_Suffix_1_rev | 5´ CTGCAGCGGCCGCTACTAGTACTAGCCTTCGATGCCGATTTTC 3´ | 59.2 |
| PhzM_SDM_PstI_1_for | 5´ CCTGGAGGATGGCCTTGTTCAATTCGCC 3´ | 69.9 |
| PhzM_SDM_PstI_1_rev | 5´ CCTCCAGGCCGAGCCCAG 3´ | 70.6 |
| PhzS_SDM_PstI_1_for | 5´ CTGGAGTTCGCCGCGATGG 3´ | 65.7 |
| PhzS_SDM_PstI_1_rev | 5´ ACTCCAGATGATCCTGCTCGCC 3´ | 65.3 |
| PhzS_SDM_PstI_2_for | 5´ GTACTGGAGGATCAACTGGTTGCG 3´ | 63.6 |
| PhzS_SDM_PstI_2_rev | 5´ GTACTGGAGGATCAACTGGTTGCG 3´ | 63.9 |
| PhzC_SDM_PstI_1_for | 5´ CTGGAGCGCTTCGCAGCG 3´ | 65.1 |
| PhzC_SDM_PstI_1_rev | 5´ CCTCCAGCAACCCGAATGG 3´ | 64.2 |
| PhzF_SDM_PstI_1_for | 5´ GCACGCCACCACCTTGCC 3´ | 66.9 |
| PhzF_SDM_PstI_1_rev | 5´ GGCGTGCAGCATGCAGCAGC 3´ | 66.9 |
| PhzB_SDM_EcoRI_1_for | 5´ GAACTCGCGGTTGCGCTTGATCTTG 3´ | 65.4 |
| PhzB_SDM_EcoRI_1_rev | 5´ GAGTTCATGAACGTCTTCCAGCAATTGCG 3´ | 64.5 |
| PhzE_SDM_EcoRI_1_for | 5´ GAACTCCATGACCTCGGC 3´ | 60.3 |
| PhzE_SDM_EcoRI_1_rev | 5´ GAGTTCCTCGACAACCGC 3´ | 60.3 |
| PhzE_SDM_PstI_1_for | 5´ CTGGAGGGCGCGCGCAGC 3´ | 71.2 |
| PhzE_SDM_PstI_1_rev | 5´ GCCCTCCAGGACCGCATCGAGATC 3´ | 70.9 |
| XylR_SDM_PstI_1_for | 5´ GAGCTCCAGACCCAGGTTG 3´ | 63.3 |
| XylR_SDM_PstI_1_rev | 5´ GTCTGGAGCTCGTAGCGCTC 3´ | 63.3 |
| XylR_Prefix_1 | 5´ GAATTCGCGGCCGCTTCTAGATGTCGCTTACATACAAACCCAAG 3´ | 57.7 |
| XylR_Suffix_1 | 5´ CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCAC 3´ | 59.8 |
| Pu_Prefix_1(After XylR) | 5´ GAATTCGCGGCCGCTTCTAGAGCGGATTCTGGGGAAAGAG 3´ | 65.0 |
| Pu_Suffix_1(After XylR) | 5´ CTGCAGCGGCCGCTACTAGTACCTTTACTGCGTGCGAGATCTCGTAGGTG 3´ | 65.1 |
| Pu_Prefix_2(Emma´s sequence) | 5´ GAATTCGCGGCCGCTTCTAGAGCCCGGGAAAGCGCGATGAAC 3´ | 66.0 |
| Pu_Suffix_2(Emma´s sequence) | 5´ CTGCAGCGGCCGCTACTAGTAGGCGTAACGGCTACGGCTGGC 3´ | 66.4 |
| DntR_Prefix_1 | 5´ GAATTCGCGGCCGCTTCTAGATGGATCTGCGCGACATCG 3´ | 61.2 |
| DntR_Suffix_1 | 5´ TACTAGTAGCGGCCGCTGCAGGCTCGAAAAGAGACTTCGTATT 3´ | 55.2 |
| DntR_Suffix_2 | 5´ CTGCAGCGGCCGCTACTAGTATTATGCTTCAGAGAAAAGCTCG 3´ | 55.3 |
| Pr_Prefix_1 | 5´ GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTG 3´ | 58.8 |
| Pr_Suffix_1 | 5´ CTGCAGCGGCCGCTACTAGTAGTTGTTTTCCTCTTGTTTTTATCGGG 3´ | 57.3 |
| p1010_for | 5´ GCTGTGTATAAGGGAGCCTGA 3´ | 59.4 |
| p1010_rev | 5´ TGACAGTTGACAGCGACAGC 3´ | 61.3 |
| Bbp_XylR_for_2 | 5´ GAATTCGCGGCCGCTTCTAGATCTCGCTTACATACAAACCC 3´ | 55.2 |
| Bbs_XylR_rev_2 | 5´ CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCACAG 3´ | 61.8 |
| Bbp_Pr_for_2 | 5´ GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTGTG 3´ | 60.8 |
| Bbs_Pr_rev_2 | 5´ CTGCAGCGGCCGCTACTAGTAGTAGGAGTACGACGTAGAACCCAAAC 3´ | 61.3 |
| Bbp_Pu_for_2 | 5´ GAATTCGCGGCCGCTTCTAGAGGGAAAGCGCGATGAACCTTTTTTATCGC 3´ | 64.3 |
| Bbs_Pu_rev_2 | 5´ CTGCAGCGGCCGCTACTAGTAGGACTCCAGGCGTAACGGCTACGG 3´ | 69.9 |
| Bbp_PhzD_for | 5´ *** 3´ | 61.0 |
| Bbs_PhzD_for | 5´ *** 3´ | 61.4 |
| Bbp_PhzA_for | 5´ *** 3´ | 63.1 |
| Bbs_PhzG_rev | 5´ *** 3´ | 61.9 |
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