Glasgow/Wetlab/Orders
From 2007.igem.org
(→[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=Glasgow <font face=georgia color=#3366CC size=4>Glasgow Parts Page</font>]) |
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(47 intermediate revisions not shown) | |||
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- | [[Glasgow|Glasgow Main Page]] | + | {| valign=top cellpadding=3 |
+ | |- | ||
+ | !align=center|[[Image:Uog.jpg]] || || [[Glasgow|<font face=georgia color=#3366CC size=4>Back To <br> Glasgow's <br> Main Page</font>]] || [[Glasgow/Wetlab|<font face=georgia color=#3366CC size=4>Back To <br> Glasgow's <br> Wetlab Log</font>]]|| [[Glasgow/Plan|<font face=georgia color=#3366CC size=4>Back To <br> Glasgow's <br> Project Page</font>]] | ||
+ | |} | ||
---- | ---- | ||
- | === Emma's | + | == [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=Glasgow <font face=georgia color=#3366CC size=4>Glasgow Parts Page</font>]== |
+ | Visit the [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=Glasgow Glasgow Parts Page] to see our constructs. | ||
+ | ---- | ||
+ | |||
+ | == <font face=georgia color=#3366CC size=4>Primers</font> == | ||
+ | |||
+ | {| border="1" cellspacing="0" cellpadding="5" align="center" | ||
+ | !Name | ||
+ | !Sequence | ||
+ | !Tm (°C) | ||
+ | |- | ||
+ | |DmpR_SDM_PstI_1_for | ||
+ | |5´ CTGGAGCAACAGCATGCGCTGTTC 3´ | ||
+ | |65.6 | ||
+ | |- | ||
+ | |DmpR_SDM_PstI_1_rev | ||
+ | |5´ GCTCCAGTTTTCAGCGATGGCC 3´ | ||
+ | |64.8 | ||
+ | |- | ||
+ | |DmpR_SDM_PstI_2_for | ||
+ | |5´ CTGGAGGAAGTGCTCAACCAGCAG 3´ | ||
+ | |66.3 | ||
+ | |- | ||
+ | |DmpR_SDM_PstI_2_rev | ||
+ | |5´ CCTCCAGCGCTTCCACCAG 3´ | ||
+ | |66.2 | ||
+ | |- | ||
+ | |DmpR_Start_Prefix_1_for | ||
+ | |5´ GAATTCGCGGCCGCTTCTAGATGCCGATCAAGTACAAGCCTG 3´ | ||
+ | |60.2 | ||
+ | |- | ||
+ | |DmpR_End_Suffix_1_rev | ||
+ | |5´ CTGCAGCGGCCGCTACTAGTACTAGCCTTCGATGCCGATTTTC 3´ | ||
+ | |59.2 | ||
+ | |- | ||
+ | |PhzM_SDM_PstI_1_for | ||
+ | |5´ CCTGGAGGATGGCCTTGTTCAATTCGCC 3´ | ||
+ | |69.9 | ||
+ | |- | ||
+ | |PhzM_SDM_PstI_1_rev | ||
+ | |5´ CCTCCAGGCCGAGCCCAG 3´ | ||
+ | |70.6 | ||
+ | |- | ||
+ | |PhzS_SDM_PstI_1_for | ||
+ | |5´ CTGGAGTTCGCCGCGATGG 3´ | ||
+ | |65.7 | ||
+ | |- | ||
+ | |PhzS_SDM_PstI_1_rev | ||
+ | |5´ ACTCCAGATGATCCTGCTCGCC 3´ | ||
+ | |65.3 | ||
+ | |- | ||
+ | |PhzS_SDM_PstI_2_for | ||
+ | |5´ GTACTGGAGGATCAACTGGTTGCG 3´ | ||
+ | |63.6 | ||
+ | |- | ||
+ | |PhzS_SDM_PstI_2_rev | ||
+ | |5´ GTACTGGAGGATCAACTGGTTGCG 3´ | ||
+ | |63.9 | ||
+ | |- | ||
+ | |PhzC_SDM_PstI_1_for | ||
+ | |5´ CTGGAGCGCTTCGCAGCG 3´ | ||
+ | |65.1 | ||
+ | |- | ||
+ | |PhzC_SDM_PstI_1_rev | ||
+ | |5´ CCTCCAGCAACCCGAATGG 3´ | ||
+ | |64.2 | ||
+ | |- | ||
+ | |PhzF_SDM_PstI_1_for | ||
+ | |5´ GCACGCCACCACCTTGCC 3´ | ||
+ | |66.9 | ||
+ | |- | ||
+ | |PhzF_SDM_PstI_1_rev | ||
+ | |5´ GGCGTGCAGCATGCAGCAGC 3´ | ||
+ | |66.9 | ||
+ | |- | ||
+ | |PhzB_SDM_EcoRI_1_for | ||
+ | |5´ GAACTCGCGGTTGCGCTTGATCTTG 3´ | ||
+ | |65.4 | ||
+ | |- | ||
+ | |PhzB_SDM_EcoRI_1_rev | ||
+ | |5´ GAGTTCATGAACGTCTTCCAGCAATTGCG 3´ | ||
+ | |64.5 | ||
+ | |- | ||
+ | |PhzE_SDM_EcoRI_1_for | ||
+ | |5´ GAACTCCATGACCTCGGC 3´ | ||
+ | |60.3 | ||
+ | |- | ||
+ | |PhzE_SDM_EcoRI_1_rev | ||
+ | |5´ GAGTTCCTCGACAACCGC 3´ | ||
+ | |60.3 | ||
+ | |- | ||
+ | |PhzE_SDM_PstI_1_for | ||
+ | |5´ CTGGAGGGCGCGCGCAGC 3´ | ||
+ | |71.2 | ||
+ | |- | ||
+ | |PhzE_SDM_PstI_1_rev | ||
+ | |5´ GCCCTCCAGGACCGCATCGAGATC 3´ | ||
+ | |70.9 | ||
+ | |- | ||
+ | |XylR_SDM_PstI_1_for | ||
+ | |5´ GAGCTCCAGACCCAGGTTG 3´ | ||
+ | |63.3 | ||
+ | |- | ||
+ | |XylR_SDM_PstI_1_rev | ||
+ | |5´ GTCTGGAGCTCGTAGCGCTC 3´ | ||
+ | |63.3 | ||
+ | |- | ||
+ | |XylR_Prefix_1 | ||
+ | |5´ GAATTCGCGGCCGCTTCTAGATGTCGCTTACATACAAACCCAAG 3´ | ||
+ | |57.7 | ||
+ | |- | ||
+ | |XylR_Suffix_1 | ||
+ | |5´ CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCAC 3´ | ||
+ | |59.8 | ||
+ | |- | ||
+ | |Pu_Prefix_1(After XylR) | ||
+ | |5´ GAATTCGCGGCCGCTTCTAGAGCGGATTCTGGGGAAAGAG 3´ | ||
+ | |65.0 | ||
+ | |- | ||
+ | |Pu_Suffix_1(After XylR) | ||
+ | |5´ CTGCAGCGGCCGCTACTAGTACCTTTACTGCGTGCGAGATCTCGTAGGTG 3´ | ||
+ | |65.1 | ||
+ | |- | ||
+ | |Pu_Prefix_2(Emma´s sequence) | ||
+ | |5´ GAATTCGCGGCCGCTTCTAGAGCCCGGGAAAGCGCGATGAAC 3´ | ||
+ | |66.0 | ||
+ | |- | ||
+ | |Pu_Suffix_2(Emma´s sequence) | ||
+ | |5´ CTGCAGCGGCCGCTACTAGTAGGCGTAACGGCTACGGCTGGC 3´ | ||
+ | |66.4 | ||
+ | |- | ||
+ | |DntR_Prefix_1 | ||
+ | |5´ GAATTCGCGGCCGCTTCTAGATGGATCTGCGCGACATCG 3´ | ||
+ | |61.2 | ||
+ | |- | ||
+ | |DntR_Suffix_1 | ||
+ | |5´ TACTAGTAGCGGCCGCTGCAGGCTCGAAAAGAGACTTCGTATT 3´ | ||
+ | |55.2 | ||
+ | |- | ||
+ | |DntR_Suffix_2 | ||
+ | |5´ CTGCAGCGGCCGCTACTAGTATTATGCTTCAGAGAAAAGCTCG 3´ | ||
+ | |55.3 | ||
+ | |- | ||
+ | |Pr_Prefix_1 | ||
+ | |5´ GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTG 3´ | ||
+ | |58.8 | ||
+ | |- | ||
+ | |Pr_Suffix_1 | ||
+ | |5´ CTGCAGCGGCCGCTACTAGTAGTTGTTTTCCTCTTGTTTTTATCGGG 3´ | ||
+ | |57.3 | ||
+ | |- | ||
+ | |p1010_for | ||
+ | |5´ GCTGTGTATAAGGGAGCCTGA 3´ | ||
+ | |59.4 | ||
+ | |- | ||
+ | |p1010_rev | ||
+ | |5´ TGACAGTTGACAGCGACAGC 3´ | ||
+ | |61.3 | ||
+ | |- | ||
+ | |Bbp_XylR_for_2 | ||
+ | |5´ GAATTCGCGGCCGCTTCTAGATCTCGCTTACATACAAACCC 3´ | ||
+ | |55.2 | ||
+ | |- | ||
+ | |Bbs_XylR_rev_2 | ||
+ | |5´ CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCACAG 3´ | ||
+ | |61.8 | ||
+ | |- | ||
+ | |Bbp_Pr_for_2 | ||
+ | |5´ GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTGTG 3´ | ||
+ | |60.8 | ||
+ | |- | ||
+ | |Bbs_Pr_rev_2 | ||
+ | |5´ CTGCAGCGGCCGCTACTAGTAGTAGGAGTACGACGTAGAACCCAAAC 3´ | ||
+ | |61.3 | ||
+ | |- | ||
+ | |Bbp_Pu_for_2 | ||
+ | |5´ GAATTCGCGGCCGCTTCTAGAGGGAAAGCGCGATGAACCTTTTTTATCGC 3´ | ||
+ | |64.3 | ||
+ | |- | ||
+ | |Bbs_Pu_rev_2 | ||
+ | |5´ CTGCAGCGGCCGCTACTAGTAGGACTCCAGGCGTAACGGCTACGG 3´ | ||
+ | |69.9 | ||
+ | |- | ||
+ | |Bbp_PhzD_for | ||
+ | |5´ GAATTCGCGGCCGCTTCTAGCACCACCGACGAGGTG 3´ | ||
+ | |61.0 | ||
+ | |- | ||
+ | |Bbs_PhzD_rev | ||
+ | |5´ CTGCAGCGGCCGCTACTAGTATCATTCCAGCACCTCGTCG 3´ | ||
+ | |61.4 | ||
+ | |- | ||
+ | |Bbp_PhzA_for | ||
+ | |5´ GAATTCGCGGCCGCTTCTAGATGAACGGTCAGCGGTACAGG 3´ | ||
+ | |63.1 | ||
+ | |- | ||
+ | |Bbs_PhzG_rev | ||
+ | |5´ CTGCAGCGGCCGCTACTAGTAGTTGCAGGTAGCGGTGCTTC 3´ | ||
+ | |61.9 | ||
+ | |} | ||
+ | ---- | ||
+ | |||
+ | == <font face=georgia color=#3366CC size=4>[[User:EmmaTravis|Emma]]'s Primers</font> == | ||
+ | {| border="1" cellspacing="0" cellpadding="5" align="center" | ||
+ | !Name | ||
+ | !Sequence | ||
+ | !Tm (°C) | ||
+ | |- | ||
+ | |* PhzUp | ||
+ | |5´ TAAGGATCCGGTAGTTCCAAGCCCCAGAAAC 3´ | ||
+ | |65.6 | ||
+ | |- | ||
+ | |* PhzLow | ||
+ | |5´ CACATTTGATCTAGATGGGTCACGGCTATTCAG 3´ | ||
+ | |64.8 | ||
+ | |- | ||
+ | |*Bbp_PhzUp | ||
+ | |5´ gaattcgcggccgcttctagaTAAGGATCCGGTAGTTCCAAGCCCCAGAAAC 3´ | ||
+ | |63.6 | ||
+ | |- | ||
+ | |*Bbs_PhzLow | ||
+ | |5´ ctgcagcggccgctactagtaCACATTTGATCTAGATGGGTCACGGCTATTCAG 3´ | ||
+ | |64.2 | ||
+ | |- | ||
+ | |Bbp_Gene | ||
+ | |5´ GAATTCGCGGCCGCTTCTAG 3´ | ||
+ | |65.7 | ||
+ | |- | ||
+ | |Bbs | ||
+ | |5´ CTGCAGCGGCCGCTACTAGTA 3´ | ||
+ | |65.3 | ||
+ | |- | ||
+ | |*Methyl_1 | ||
+ | |5´ TTTTTGAATTCTTTCGGACGCAGGAAAAG 3´ | ||
+ | |66.3 | ||
+ | |- | ||
+ | |*Methyl_2 | ||
+ | |5´ TTTTTGGATCCGTTGAAAGTTCCGATTCA 3´ | ||
+ | |66.2 | ||
+ | |- | ||
+ | |*Bbp_Methyl_1 | ||
+ | |5´ gaattcgcggccgcttctagaTTTTTGAATTCTTTCGGACGCAGGAAAAG 3´ | ||
+ | |63.9 | ||
+ | |- | ||
+ | |*Bbs_Methyl_2 | ||
+ | |5´ ctgcagcggccgctactagtaTTTTTGGATCCGTTGAAAGTTCCGATTCA 3´ | ||
+ | |66.9 | ||
+ | |- | ||
+ | |PhzM_for_1 | ||
+ | |5´ atgaataattcgaatcttgctgct 3´ | ||
+ | |65.4 | ||
+ | |- | ||
+ | |PhzM_rev_1 | ||
+ | |5´ tcaggccctggcagcgac 3´ | ||
+ | |60.3 | ||
+ | |- | ||
+ | |Bbp_PhzM_for_1 | ||
+ | |5´ gaattcgcggccgcttctagatgaataattcgaatcttgctgct 3´ | ||
+ | |64.5 | ||
+ | |- | ||
+ | |Bbs_PhzM_rev_1 | ||
+ | |5´ ctgcagcggccgctactagtatcaggccctggcagcgac 3´ | ||
+ | |60.3 | ||
+ | |- | ||
+ | |*Oxy_1 | ||
+ | |5´TCGAACACTCTAGAAAAGGAAGCACC 3´ | ||
+ | |60.2 | ||
+ | |- | ||
+ | |*Oxy_2 | ||
+ | |5´ TTTTTTGCATGCTAGCGTGGCCGTTCC 3´ | ||
+ | |59.2 | ||
+ | |- | ||
+ | |*Bbp_Oxy_1 | ||
+ | |5´ gaattcgcggccgcttctagaTCGAACACTCTAGAAAAGGAAGCACC 3´ | ||
+ | |65.1 | ||
+ | |- | ||
+ | |*Bbs_Oxy_2 | ||
+ | |5´ ctgcagcggccgctactagtaTTTTTTGCATGCTAGCGTGGCCGTTCC 3´ | ||
+ | |66.9 | ||
+ | |- | ||
+ | |PhzS_for_1 | ||
+ | |5´ atgagcgaacccatcgatat 3´ | ||
+ | |71.2 | ||
+ | |- | ||
+ | |PhzS_rev_1 | ||
+ | |5´ ctagcgtggccgttccacctg 3´ | ||
+ | |60.2 | ||
+ | |- | ||
+ | |Bbp_PhzS_for_1 | ||
+ | |5´ gaattcgcggccgcttctagatgagcgaacccatcgatat 3´ | ||
+ | |70.9 | ||
+ | |- | ||
+ | |Bbs_PhzS_rev_1 | ||
+ | |5´ ctgcagcggccgctactagtactagcgtggccgttccacctg 3´ | ||
+ | |63.3 | ||
+ | |- | ||
+ | |PhzH_up | ||
+ | |5´ CGCACGGATCCTTTCAGAATGTTC 3´ | ||
+ | |69.9 | ||
+ | |- | ||
+ | |PhzH_low | ||
+ | |5´ GCCACGCCAAGCTTCACGCTCA 3´ | ||
+ | |70.6 | ||
+ | |- | ||
+ | |Bbp_PhzUp | ||
+ | |5´ gaattcgcggccgcttctagaTAAGGATCCGGTAGTTCCAAGCCCCAGAAAC 3´ | ||
+ | |63.3 | ||
+ | |- | ||
+ | |Bbs_PhzLow | ||
+ | |5´ ctgcagcggccgctactagtaCACATTTGATCTAGATGGGTCACGGCTATTCAG 3´ | ||
+ | |57.7 | ||
+ | |} | ||
+ | ---- | ||
+ | {| valign=top cellpadding=3 | ||
+ | |- | ||
+ | !align=center|[[Image:Uog.jpg]] || || [[Glasgow|<font face=georgia color=#3366CC size=4>Back To <br> Glasgow's <br> Main Page</font>]] || [[Glasgow/Wetlab|<font face=georgia color=#3366CC size=4>Back To <br> Glasgow's <br> Wetlab Log</font>]] || [[Glasgow/Plan|<font face=georgia color=#3366CC size=4>Back To <br> Glasgow's <br> Project Page</font>]] | ||
+ | |} |
Latest revision as of 11:28, 26 October 2007
![]() | Back To Glasgow's Main Page | Back To Glasgow's Wetlab Log | Back To Glasgow's Project Page |
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[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=Glasgow Glasgow Parts Page]
Visit the [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=Glasgow Glasgow Parts Page] to see our constructs.
Primers
Name | Sequence | Tm (°C) |
---|---|---|
DmpR_SDM_PstI_1_for | 5´ CTGGAGCAACAGCATGCGCTGTTC 3´ | 65.6 |
DmpR_SDM_PstI_1_rev | 5´ GCTCCAGTTTTCAGCGATGGCC 3´ | 64.8 |
DmpR_SDM_PstI_2_for | 5´ CTGGAGGAAGTGCTCAACCAGCAG 3´ | 66.3 |
DmpR_SDM_PstI_2_rev | 5´ CCTCCAGCGCTTCCACCAG 3´ | 66.2 |
DmpR_Start_Prefix_1_for | 5´ GAATTCGCGGCCGCTTCTAGATGCCGATCAAGTACAAGCCTG 3´ | 60.2 |
DmpR_End_Suffix_1_rev | 5´ CTGCAGCGGCCGCTACTAGTACTAGCCTTCGATGCCGATTTTC 3´ | 59.2 |
PhzM_SDM_PstI_1_for | 5´ CCTGGAGGATGGCCTTGTTCAATTCGCC 3´ | 69.9 |
PhzM_SDM_PstI_1_rev | 5´ CCTCCAGGCCGAGCCCAG 3´ | 70.6 |
PhzS_SDM_PstI_1_for | 5´ CTGGAGTTCGCCGCGATGG 3´ | 65.7 |
PhzS_SDM_PstI_1_rev | 5´ ACTCCAGATGATCCTGCTCGCC 3´ | 65.3 |
PhzS_SDM_PstI_2_for | 5´ GTACTGGAGGATCAACTGGTTGCG 3´ | 63.6 |
PhzS_SDM_PstI_2_rev | 5´ GTACTGGAGGATCAACTGGTTGCG 3´ | 63.9 |
PhzC_SDM_PstI_1_for | 5´ CTGGAGCGCTTCGCAGCG 3´ | 65.1 |
PhzC_SDM_PstI_1_rev | 5´ CCTCCAGCAACCCGAATGG 3´ | 64.2 |
PhzF_SDM_PstI_1_for | 5´ GCACGCCACCACCTTGCC 3´ | 66.9 |
PhzF_SDM_PstI_1_rev | 5´ GGCGTGCAGCATGCAGCAGC 3´ | 66.9 |
PhzB_SDM_EcoRI_1_for | 5´ GAACTCGCGGTTGCGCTTGATCTTG 3´ | 65.4 |
PhzB_SDM_EcoRI_1_rev | 5´ GAGTTCATGAACGTCTTCCAGCAATTGCG 3´ | 64.5 |
PhzE_SDM_EcoRI_1_for | 5´ GAACTCCATGACCTCGGC 3´ | 60.3 |
PhzE_SDM_EcoRI_1_rev | 5´ GAGTTCCTCGACAACCGC 3´ | 60.3 |
PhzE_SDM_PstI_1_for | 5´ CTGGAGGGCGCGCGCAGC 3´ | 71.2 |
PhzE_SDM_PstI_1_rev | 5´ GCCCTCCAGGACCGCATCGAGATC 3´ | 70.9 |
XylR_SDM_PstI_1_for | 5´ GAGCTCCAGACCCAGGTTG 3´ | 63.3 |
XylR_SDM_PstI_1_rev | 5´ GTCTGGAGCTCGTAGCGCTC 3´ | 63.3 |
XylR_Prefix_1 | 5´ GAATTCGCGGCCGCTTCTAGATGTCGCTTACATACAAACCCAAG 3´ | 57.7 |
XylR_Suffix_1 | 5´ CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCAC 3´ | 59.8 |
Pu_Prefix_1(After XylR) | 5´ GAATTCGCGGCCGCTTCTAGAGCGGATTCTGGGGAAAGAG 3´ | 65.0 |
Pu_Suffix_1(After XylR) | 5´ CTGCAGCGGCCGCTACTAGTACCTTTACTGCGTGCGAGATCTCGTAGGTG 3´ | 65.1 |
Pu_Prefix_2(Emma´s sequence) | 5´ GAATTCGCGGCCGCTTCTAGAGCCCGGGAAAGCGCGATGAAC 3´ | 66.0 |
Pu_Suffix_2(Emma´s sequence) | 5´ CTGCAGCGGCCGCTACTAGTAGGCGTAACGGCTACGGCTGGC 3´ | 66.4 |
DntR_Prefix_1 | 5´ GAATTCGCGGCCGCTTCTAGATGGATCTGCGCGACATCG 3´ | 61.2 |
DntR_Suffix_1 | 5´ TACTAGTAGCGGCCGCTGCAGGCTCGAAAAGAGACTTCGTATT 3´ | 55.2 |
DntR_Suffix_2 | 5´ CTGCAGCGGCCGCTACTAGTATTATGCTTCAGAGAAAAGCTCG 3´ | 55.3 |
Pr_Prefix_1 | 5´ GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTG 3´ | 58.8 |
Pr_Suffix_1 | 5´ CTGCAGCGGCCGCTACTAGTAGTTGTTTTCCTCTTGTTTTTATCGGG 3´ | 57.3 |
p1010_for | 5´ GCTGTGTATAAGGGAGCCTGA 3´ | 59.4 |
p1010_rev | 5´ TGACAGTTGACAGCGACAGC 3´ | 61.3 |
Bbp_XylR_for_2 | 5´ GAATTCGCGGCCGCTTCTAGATCTCGCTTACATACAAACCC 3´ | 55.2 |
Bbs_XylR_rev_2 | 5´ CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCACAG 3´ | 61.8 |
Bbp_Pr_for_2 | 5´ GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTGTG 3´ | 60.8 |
Bbs_Pr_rev_2 | 5´ CTGCAGCGGCCGCTACTAGTAGTAGGAGTACGACGTAGAACCCAAAC 3´ | 61.3 |
Bbp_Pu_for_2 | 5´ GAATTCGCGGCCGCTTCTAGAGGGAAAGCGCGATGAACCTTTTTTATCGC 3´ | 64.3 |
Bbs_Pu_rev_2 | 5´ CTGCAGCGGCCGCTACTAGTAGGACTCCAGGCGTAACGGCTACGG 3´ | 69.9 |
Bbp_PhzD_for | 5´ GAATTCGCGGCCGCTTCTAGCACCACCGACGAGGTG 3´ | 61.0 |
Bbs_PhzD_rev | 5´ CTGCAGCGGCCGCTACTAGTATCATTCCAGCACCTCGTCG 3´ | 61.4 |
Bbp_PhzA_for | 5´ GAATTCGCGGCCGCTTCTAGATGAACGGTCAGCGGTACAGG 3´ | 63.1 |
Bbs_PhzG_rev | 5´ CTGCAGCGGCCGCTACTAGTAGTTGCAGGTAGCGGTGCTTC 3´ | 61.9 |
Emma's Primers
Name | Sequence | Tm (°C) |
---|---|---|
* PhzUp | 5´ TAAGGATCCGGTAGTTCCAAGCCCCAGAAAC 3´ | 65.6 |
* PhzLow | 5´ CACATTTGATCTAGATGGGTCACGGCTATTCAG 3´ | 64.8 |
*Bbp_PhzUp | 5´ gaattcgcggccgcttctagaTAAGGATCCGGTAGTTCCAAGCCCCAGAAAC 3´ | 63.6 |
*Bbs_PhzLow | 5´ ctgcagcggccgctactagtaCACATTTGATCTAGATGGGTCACGGCTATTCAG 3´ | 64.2 |
Bbp_Gene | 5´ GAATTCGCGGCCGCTTCTAG 3´ | 65.7 |
Bbs | 5´ CTGCAGCGGCCGCTACTAGTA 3´ | 65.3 |
*Methyl_1 | 5´ TTTTTGAATTCTTTCGGACGCAGGAAAAG 3´ | 66.3 |
*Methyl_2 | 5´ TTTTTGGATCCGTTGAAAGTTCCGATTCA 3´ | 66.2 |
*Bbp_Methyl_1 | 5´ gaattcgcggccgcttctagaTTTTTGAATTCTTTCGGACGCAGGAAAAG 3´ | 63.9 |
*Bbs_Methyl_2 | 5´ ctgcagcggccgctactagtaTTTTTGGATCCGTTGAAAGTTCCGATTCA 3´ | 66.9 |
PhzM_for_1 | 5´ atgaataattcgaatcttgctgct 3´ | 65.4 |
PhzM_rev_1 | 5´ tcaggccctggcagcgac 3´ | 60.3 |
Bbp_PhzM_for_1 | 5´ gaattcgcggccgcttctagatgaataattcgaatcttgctgct 3´ | 64.5 |
Bbs_PhzM_rev_1 | 5´ ctgcagcggccgctactagtatcaggccctggcagcgac 3´ | 60.3 |
*Oxy_1 | 5´TCGAACACTCTAGAAAAGGAAGCACC 3´ | 60.2 |
*Oxy_2 | 5´ TTTTTTGCATGCTAGCGTGGCCGTTCC 3´ | 59.2 |
*Bbp_Oxy_1 | 5´ gaattcgcggccgcttctagaTCGAACACTCTAGAAAAGGAAGCACC 3´ | 65.1 |
*Bbs_Oxy_2 | 5´ ctgcagcggccgctactagtaTTTTTTGCATGCTAGCGTGGCCGTTCC 3´ | 66.9 |
PhzS_for_1 | 5´ atgagcgaacccatcgatat 3´ | 71.2 |
PhzS_rev_1 | 5´ ctagcgtggccgttccacctg 3´ | 60.2 |
Bbp_PhzS_for_1 | 5´ gaattcgcggccgcttctagatgagcgaacccatcgatat 3´ | 70.9 |
Bbs_PhzS_rev_1 | 5´ ctgcagcggccgctactagtactagcgtggccgttccacctg 3´ | 63.3 |
PhzH_up | 5´ CGCACGGATCCTTTCAGAATGTTC 3´ | 69.9 |
PhzH_low | 5´ GCCACGCCAAGCTTCACGCTCA 3´ | 70.6 |
Bbp_PhzUp | 5´ gaattcgcggccgcttctagaTAAGGATCCGGTAGTTCCAAGCCCCAGAAAC 3´ | 63.3 |
Bbs_PhzLow | 5´ ctgcagcggccgctactagtaCACATTTGATCTAGATGGGTCACGGCTATTCAG 3´ | 57.7 |
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