Week 14

From 2007.igem.org

(Difference between revisions)

Latest revision as of 15:51, 26 October 2007

10/01/07

Meeting: Ethical issue.
Work on image segmentation algorithm.

10/02/07

Digestion of [http://partsregistry.org/Part:pSB4A3 pSB4A3];
Run on electrophoresis gel.
[http://partsregistry.org/Part:pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763033 I763033] band extraction.
Ligations for:

-[http://partsregistry.org/Part:BBa_I763004 I763004]+[http://partsregistry.org/Part:BBa_I763033 I763033];

-[http://partsregistry.org/Part:BBa_I763012 I763012]+[http://partsregistry.org/Part:BBa_I763020 I763020][http://partsregistry.org/Part:BBa_I763032 (I763032)].

10/03/07

Digestions of:

-[http://partsregistry.org/Part:BBa_I763032 I763032] with Eco/Pst1;

-[http://partsregistry.org/Part:BBa_I763031 I763031] with Eco/Pst1;

-[http://partsregistry.org/Part:BBa_I763036 I763036] with Eco/Pst1;

-[http://partsregistry.org/Part:pSB4A3 pSB4A3] with Eco/Pst1.

Run on electrophoresis gel.
[http://partsregistry.org/Part:BBa_I763031 I763031], [http://partsregistry.org/Part:BBa_I763036 I763036] and [http://partsregistry.org/Part:pSB4A3 pSB4A3] band extraction.
Transformations of:

-[http://partsregistry.org/Part:BBa_I763034 I763034];

-[http://partsregistry.org/Part:BBa_I763031 I763031];

-[http://partsregistry.org/Part:BBa_I763032 I763032].

Ligations for:

-[http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];

-[http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];

Experimental Setup

To have an higher precision for our measurements, we have decided to get, for the same field of view on the same sample of bacteria solution, either a short film segment (from which we can extract frames) and a Microsoft Excel file, which contains a ten seconds interval of values from the photomultiplier output, taken at a 100Hz frequency.

In order to have the same focus for the microscope's oculars, the photocamera and the photomultiplier we have to calibrate our system by changing the position of both camera and photomultiplier.

Finally, we check that the photomultiplier field of view is the same size as the microscope's one: by moving the appropiate diaphragms, we adjust the previously done settings.

10/04/07

Trasformation of:

-[http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];

-[http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];

We inoculate [http://partsregistry.org/Part:BBa_I763034 I763034], [http://partsregistry.org/Part:BBa_I763031 I763031] + [http://partsregistry.org/Part:pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3] (in 6 ml).

10/05/07

Stock preparation (500 ul) of [http://partsregistry.org/Part:I763034 I763034],[http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part:BBa_pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:BBa_pSB4A3 pSB4A3].
Miniprep of [http://partsregistry.org/Part:BBa_I763034 I763034], [http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part:BBa_pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:BBa_pSB4A3 pSB4A3].
Control digestion of:

-[http://partsregistry.org/Part:BBa_I763034 I763034] with Xba/Pst1;

-[http://partsregistry.org/Part:BBa_I763031 I763031] with Eco/Xba and Xba/Pst1;

-[http://partsregistry.org/Part:BBa_I763032 I763032] with Eco/Xba and Xba/Pst1.

Run on electrophoresis gel and [http://partsregistry.org/Part:BBa_I763032 I763032] (Eco/Xba and Xba/Pst1) band extraction.

Back

@@ Line 1: / Line 1: @@
 ::'''10/01/07'''
 *Meeting: Ethical issue.
+*Work on image segmentation algorithm.
 ::'''10/02/07'''
-*Digestion of PSB4A3;
+*Digestion of [http://partsregistry.org/Part:pSB4A3 pSB4A3];
 *Run on electrophoresis gel.
-*pSB4A3 and Ptet-RFP band extraction.
+*[http://partsregistry.org/Part:pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763033 I763033] band extraction.
-*Ligation for
+*Ligations for:
-::-pLac-GFP+I76303;
+::-[http://partsregistry.org/Part:BBa_I763004 I763004]+[http://partsregistry.org/Part:BBa_I763033 I763033];
-::-I763012+I763020(I763032).
+::-[http://partsregistry.org/Part:BBa_I763012 I763012]+[http://partsregistry.org/Part:BBa_I763020 I763020][http://partsregistry.org/Part:BBa_I763032 (I763032)].
@@ Line 16: / Line 16: @@
 ::'''10/03/07'''
-*Digestion of:
+*Digestions of:
-::-pLac-Lacy-GFP with Eco/Pst1;
+::-[http://partsregistry.org/Part:BBa_I763032 I763032] with Eco/Pst1;
-::-I763031 with Eco/Pst1;
+::-[http://partsregistry.org/Part:BBa_I763031 I763031] with Eco/Pst1;
-::-I763036 with Eco/Pst1;
+::-[http://partsregistry.org/Part:BBa_I763036 I763036] with Eco/Pst1;
-::-pSB4A3 with Eco/Pst1.
+::-[http://partsregistry.org/Part:pSB4A3 pSB4A3] with Eco/Pst1.
 *Run on electrophoresis gel.
-*I763031, I763036 and pSB4A3 band extraction.
+*[http://partsregistry.org/Part:BBa_I763031 I763031], [http://partsregistry.org/Part:BBa_I763036 I763036] and [http://partsregistry.org/Part:pSB4A3 pSB4A3] band extraction.
-*Transformation of
+*Transformations of:
-::-pTet-RFP-Plac GFP;
+::-[http://partsregistry.org/Part:BBa_I763034 I763034];
-::-I763031;
+::-[http://partsregistry.org/Part:BBa_I763031 I763031];
-::-I763032.
+::-[http://partsregistry.org/Part:BBa_I763032 I763032].
-*Ligation for
+*Ligations for:
-::-I763031+pSB4A3;
+::-[http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
-::-I763032+pSB4A3.
+::-[http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
 '''Experimental Setup'''
@@ Line 42: / Line 42: @@
 ::'''10/04/07'''
-*Trasformation of
+*Trasformation of:
-::-I763031+pSB4A3;
+::-[http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
-::-I763032+pSB4A3.
+::-[http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
-*We inoculate I763034,I763031+pSB4A3 and I763032+pSB4A3 (in 6 ml).
+*We inoculate [http://partsregistry.org/Part:BBa_I763034 I763034], [http://partsregistry.org/Part:BBa_I763031 I763031] + [http://partsregistry.org/Part:pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3] (in 6 ml).
@@ Line 51: / Line 51: @@
 ::'''10/05/07'''
-*Stock preparation (500 ul) of I763034,I763031+pSB4A3 and I763032+pSB4A3.
+*Stock preparation (500 ul) of [http://partsregistry.org/Part:I763034 I763034],[http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part:BBa_pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:BBa_pSB4A3 pSB4A3].
-*Miniprep of I763034,I763031+pSB4A3 and I763032+pSB4A3.
+*Miniprep of [http://partsregistry.org/Part:BBa_I763034 I763034], [http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part:BBa_pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:BBa_pSB4A3 pSB4A3].
-*Control digestion of
+*Control digestion of:
-::-I763034 with Xba/Pst1;
+::-[http://partsregistry.org/Part:BBa_I763034 I763034] with Xba/Pst1;
-::-I763031 with Eco/Xba and Xba/Pst1;
+::-[http://partsregistry.org/Part:BBa_I763031 I763031] with Eco/Xba and Xba/Pst1;
-::-I763032 with Eco/Xba and Xba/Pst1.
+::-[http://partsregistry.org/Part:BBa_I763032 I763032] with Eco/Xba and Xba/Pst1.
-*Run on electrophoresis gel and I763032(Eco/Xba and Xba/Pst1) band extraction.
+*Run on electrophoresis gel and [http://partsregistry.org/Part:BBa_I763032 I763032] (Eco/Xba and Xba/Pst1) band extraction.
-[[Bologna | Back]]
+[[Bologna#Diary | Back]]

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Week 14

From 2007.igem.org

Latest revision as of 15:51, 26 October 2007