Berkeley LBL/MimiNotebook
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== '''Construction of pET3A Derivatives Containing R-bchH, R-bchI, and R-bchD''' (Simultaneous Ligation) == | == '''Construction of pET3A Derivatives Containing R-bchH, R-bchI, and R-bchD''' (Simultaneous Ligation) == | ||
| + | |||
| + | |||
| + | '''''Rhodobacter sphaeroides''''' | ||
| + | '''R-bchH:''' 3582 base pairs | ||
| + | '''R-bchI''': 1005 base pairs | ||
| + | '''R-bchD''': 1695 base pairs | ||
| + | |||
| + | [[Berkeley_LBL/MimiSequence2|Sequences and Properties of Oligonucleotides for R-bch Genes]] | ||
| + | |||
| + | [[Berkeley_LBL/Mimi_RbchHID|'''Construction of ''pET3A-(R)-bchHID''''']] | ||
| + | - Not Successful | ||
| + | |||
| + | [[Berkeley_LBL/MimiSequence3|Sequences and Properties of Oligonucleotides for R-bch Genes(NEW)]] | ||
| + | |||
| + | [[Berkeley_LBL/Mimi_RbchHID2|'''Construction of ''pET3A-(R)-bchHID''' - 2nd Attempt'']] | ||
| + | - Not Successful | ||
| + | |||
| + | |||
== '''Contruction of pET3a Derivatives Containing S-chlH, S-chlI, and S-chlD''' (Sequential Ligation) == | == '''Contruction of pET3a Derivatives Containing S-chlH, S-chlI, and S-chlD''' (Sequential Ligation) == | ||
| - | '''''Cyanobacteria | + | '''''Cyanobacteria Synechocystis''''' |
'''S-chlH:''' 3996 base pairs | '''S-chlH:''' 3996 base pairs | ||
'''S-chlI''': 1074 base pairs | '''S-chlI''': 1074 base pairs | ||
'''S-chlD''': 2031 base pairs | '''S-chlD''': 2031 base pairs | ||
| - | [[Berkeley_LBL/MimiSequence|Sequences and Properties of Oligonucleotides]] | + | [[Berkeley_LBL/MimiSequence|Sequences and Properties of Oligonucleotides for S-chl Genes]] |
[[Berkeley_LBL/Mimi-SchlH|'''Construction of ''pET3A-(S)-chlH''''']] | [[Berkeley_LBL/Mimi-SchlH|'''Construction of ''pET3A-(S)-chlH''''']] | ||
Latest revision as of 20:29, 26 October 2007
| Home | Project Description | Methods | Notebook | Results and Discussion | Resources |
Construction of pET3A Derivatives Containing R-bchH, R-bchI, and R-bchD (Simultaneous Ligation)
Rhodobacter sphaeroides
R-bchH: 3582 base pairs
R-bchI: 1005 base pairs
R-bchD: 1695 base pairs
Sequences and Properties of Oligonucleotides for R-bch Genes
Construction of pET3A-(R)-bchHID - Not Successful
Sequences and Properties of Oligonucleotides for R-bch Genes(NEW)
Construction of pET3A-(R)-bchHID - 2nd Attempt - Not Successful
Contruction of pET3a Derivatives Containing S-chlH, S-chlI, and S-chlD (Sequential Ligation)
Cyanobacteria Synechocystis
S-chlH: 3996 base pairs
S-chlI: 1074 base pairs
S-chlD: 2031 base pairs
Sequences and Properties of Oligonucleotides for S-chl Genes
Construction of pET3A-(S)-chlH
Construction of pET3A-(S)-chlHI
Construction of pET3A-(S)-chlHID
