Paris/October 19

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[[Paris/October 18|yesterday]] -- [[Paris/October 20|tomorrow]] <br>
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== FACS ==
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== PCR reactions ==
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== Digestion reactions ==
== Digestion reactions ==
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|pSB1A2 vector digested with EcoRI and PstI enzymes
|pSB1A2 vector digested with EcoRI and PstI enzymes
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |D80
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|PacI[ptet>>lox71-gfpT-lox66-mrfp]SmaI
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|P15
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|PacI
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|SmaI
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|ptet>>lox71-gfpT-lox66-mrfp P15 PCR product digested by PacI-SmaI for cloning into pY-2C-IntC
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |D81
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|PacI[pY-2C-IntC]SmaI
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|pY-2C-IntC plasmid
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|PacI
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|SmaI
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|pY-2C-IntC digested for ptet>>lox71-gfpT-lox66-mrfp cloning
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|rbsdapAsubtilis extracted for use as a BI
|rbsdapAsubtilis extracted for use as a BI
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More digestions that need to be performed
 
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{|
 
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|- style="background: #ccccff;"
 
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! colspan="5" style="background: #ccccff;" | Digestion Products
 
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|- style="background: #ccccff; text-align: center;"
 
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|width=5%| Number
 
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|width=24%| Product Name
 
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|width=24%| Matrix Name
 
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|width=5%| Enzyme 1
 
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|width=5%| Enzyme 2
 
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|width=5%| Size
 
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|width=34%| Description
 
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|width=1%|
 
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|width=1%|
 
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|width=1%|
 
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|- style="background: #cccccc;" 
 
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| style="background: #ccffcc;" |D76
 
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|DGAT BB
 
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|P14(19/10/07)
 
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|EcoRI
 
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|PstI
 
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|DGAT (delta PstI)
 
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|- style="background: #cccccc;" 
 
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| style="background: #ccffcc;" |D79
 
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|DGAT BI
 
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|P14(19/10/07)
 
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|XbaI
 
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|PstI
 
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|DGAT(delta PstI)PCR product for cloming as a BB in D47 
 
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|- style="background: #cccccc;" 
 
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| style="background: #ccffcc;" |D80
 
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|PacI[ptet>>lox71-gfpT-lox66-mrfp]SmaI
 
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|P15
 
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|PacI
 
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|SmaI
 
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|
 
-
|ptet>>lox71-gfpT-lox66-mrfp P15 PCR product digested by PacI-SmaI for cloning into pY-2C-IntC
 
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|
 
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|
 
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|
 
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|- style="background: #cccccc;" 
 
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| style="background: #ccffcc;" |D81
 
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|PacI[pY-2C-IntC]SmaI
 
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|pY-2C-IntC plasmid
 
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|PacI
 
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|SmaI
 
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|
 
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|pY-2C-IntC digested for ptet>>lox71-gfpT-lox66-mrfp cloning
 
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|- style="background: #cccccc;" 
 
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| style="background: #ccffcc;" |D82
 
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|frt1-CmR-frt2 FI
 
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|P16
 
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|EcoRI
 
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|SpeI
 
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|frt1-CmR-frt2 PCR product digested for use as a FI
 
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|- style="background: #cccccc;" 
 
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| style="background: #ccffcc;" |D83
 
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|frt1-CmR-frt2 BI
 
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|P16
 
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|XbaI
 
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|PstI
 
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|
 
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|frt1-CmR-frt2 PCR product digested for use as a BI
 
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|- style="background: #cccccc;" 
 
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| style="background: #ccffcc;" |D84
 
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|frt1-CmR-frt2 BBi
 
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|P16
 
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|EcoRI
 
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|PstI
 
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|
 
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|frt1-CmR-frt2 PCR product digested for cloning as a BB
 
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|- style="background: #cccccc;" 
 
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| style="background: #ccffcc;" |D85
 
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|frt1-CmR-frt2-T1T2 FI
 
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|P17
 
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|EcoRI
 
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|SpeI
 
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|
 
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|frt1-CmR-frt2-T1T2 PCR product digested for use as a FI
 
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|- style="background: #cccccc;" 
 
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| style="background: #ccffcc;" |D86
 
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|frt1-CmR-frt2-T1T2 BI
 
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|P17
 
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|XbaI-
 
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|PstI
 
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|frt1-CmR-frt2-T1T2 PCR product digested for use as a BI
 
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|- style="background: #cccccc;" 
 
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| style="background: #ccffcc;" |D87
 
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|frt1-CmR-frt2-T1T2 BBi
 
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|P17
 
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|EcoRI
 
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|PstI
 
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|
 
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|frt1-CmR-frt2-T1T2 PCR product digested for cloning as a BB
 
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Latest revision as of 23:38, 26 October 2007

yesterday -- tomorrow

FACS

PCR reactions

Digestion reactions

Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 Size Description
D22bis pSB1A2 BBv MP6.1 et .2 EcoRI PstI pSB1A2 vector digested with EcoRI and PstI enzymes
D80 PacI[ptet>>lox71-gfpT-lox66-mrfp]SmaI P15 PacI SmaI ptet>>lox71-gfpT-lox66-mrfp P15 PCR product digested by PacI-SmaI for cloning into pY-2C-IntC
D81 PacI[pY-2C-IntC]SmaI pY-2C-IntC plasmid PacI SmaI pY-2C-IntC digested for ptet>>lox71-gfpT-lox66-mrfp cloning
D88 pTet>>lox71-gfptripqrt FV L34.1 et .2 EcoRI XbaI pTet>>lox71-gfptripqrt pSB1A2 dig. for use as a FV
D89 T-lox66-rbsdapAcoli BV L53.7 SpeI PstI T-lox66-rbsdapAcoli pSB1A2 dig. for use as a BV
D90 T-lox66-rbsdapAsubtilis BV L56 SpeI PstI T-lox66-rbsdapAsubtilis pSB1A2 dig. for use as a BV
D91 ptet>>lox71-gfpT-lox66-mrfp pSB1A2 BV L59.6, .7 SpeI PstI ptet>>lox71-gfpT-lox66-mrfp pSB1A2 dig. for use as a BV
D92 rbsdapAcoli BI L39.1 XbaI PstI rbsdapAcoli extracted for use as a BI
D93 rbsdapAsubtilis BI L64.1, .2 XbaI PstI rbsdapAsubtilis extracted for use as a BI
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