Glasgow/Wetlab
From 2007.igem.org
< Glasgow(Difference between revisions)
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- | [[Glasgow|Glasgow Main Page]] | + | {| valign=top cellpadding=3 |
+ | |- | ||
+ | !align=center|[[Image:Uog.jpg]] || || [[Glasgow|<font face=georgia color=#3366CC size=4>Back To <br> Glasgow's <br> Main Page</font>]] || [[Glasgow/Modeling|<font face=georgia color=#3366CC size=4>Go To <br> Glasgow's <br> Modelling Page</font>]] | ||
+ | |} | ||
---- | ---- | ||
+ | {|cellspacing="6px" cellpadding="16" border="0" width="100%" | ||
+ | |- align=center | ||
+ | |[https://2007.igem.org/Glasgow/Wetlab/Protocols <font face=georgia color=#3366CC size=5><b>Protocols</b></font>] | ||
+ | |[https://2007.igem.org/Glasgow/Wetlab/References <font face=georgia color=#3366CC size=5><b>References</b></font>] | ||
+ | |[https://2007.igem.org/Glasgow/Wetlab/Resources <font face=georgia color=#3366CC size=5><b>Resources</b></font>] | ||
+ | |[https://2007.igem.org/Glasgow/Wetlab/Orders <font face=georgia color=#3366CC size=5><b>Sequences</b></font>] | ||
+ | |[https://2007.igem.org/Glasgow/Wetlab/Biobricks <font face=georgia color=#3366CC size=5><b>Biobricks<br>Used</b></font>] | ||
+ | |[https://2007.igem.org/Glasgow/Wetlab/Gels <font face=georgia color=#3366CC size=5><b>Gels</b></font>] | ||
+ | |} | ||
+ | ---- | ||
''Click on a week number for more detailed lab book...'' | ''Click on a week number for more detailed lab book...'' | ||
+ | |||
{|cellspacing="6px" cellpadding="16" border="0" width="100%" | {|cellspacing="6px" cellpadding="16" border="0" width="100%" | ||
- | |- align=" | + | |- align="center" |
- | !width=" | + | !width="50%" | [https://2007.igem.org/Glasgow/Wetlab/Week1 <font face=georgia color=#3366CC size=7><b>Week 1</b></font>] <br> |
- | + | !width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week2 <font face=georgia color=#3366CC size=7><b>Week 2</b></font>] <br> | |
- | !width=" | + | |
- | |- align=" | + | |- valign=top |
- | !width=" | + | | |
- | + | *'''First selection of biobricks transformed.''' | |
- | !width=" | + | *'''Preparation of stocks.''' |
- | |- align=" | + | | |
- | !width=" | + | *'''Researched DmpR, XylR and DntR sensor systems.''' |
- | + | *'''Designed primers for DmpR, DntR, XylR and associated promoters.''' | |
- | !width=" | + | |
- | |- align=" | + | |- align="center" |
- | !width=" | + | !width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week3 <font face=georgia color=#3366CC size=7><b>Week 3</b></font>] <br> |
- | + | ||
- | !width=" | + | !width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week4 <font face=georgia color=#3366CC size=7><b>Week 4</b></font>] <br> |
- | |- align=" | + | |
- | !width=" | + | |- valign=top |
- | + | | | |
- | !width=" | + | *'''BioBricks tested by digestion and PCR.''' |
+ | *'''PCR with new primers associated with DntR and XylR.''' | ||
+ | | | ||
+ | * '''phzM and phzS cloned into TOPO vectors.''' | ||
+ | *'''Primer design for 7 gene operon.''' | ||
+ | *'''DntR cloned into TOPO vector.''' | ||
+ | *'''Primer design for XylR and associated genes.''' | ||
+ | *'''Death gene cloned into TOPO vector.''' | ||
+ | *'''Primer design for death gene.''' | ||
+ | |||
+ | |- align="center" | ||
+ | !width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week5 <font face=georgia color=#3366CC size=7><b>Week 5</b></font>] <br> | ||
+ | |||
+ | !width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week6 <font face=georgia color=#3366CC size=7><b>Week 6</b></font>] <br> | ||
+ | |||
+ | |- valign=top | ||
+ | | | ||
+ | *'''Suspected amplification of 7 gene operon.''' | ||
+ | * '''phzM and phzS confirmed as present in TOPO vectors.''' | ||
+ | *'''Confirmed presence of death gene plasmid in some vectors.''' | ||
+ | *'''Miller assay performed with DntR system.''' | ||
+ | *'''Yeast cells.''' | ||
+ | | | ||
+ | *'''Biobricks made of phzM, phzS, and DntR.''' | ||
+ | |||
+ | |- align="center" | ||
+ | !width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week7 <font face=georgia color=#3366CC size=7><b>Week 7</b></font>] <br> | ||
+ | |||
+ | !width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week8 <font face=georgia color=#3366CC size=7><b>Week 8</b></font>] <br> | ||
+ | |||
+ | |- valign=top | ||
+ | | | ||
+ | *'''Utilised new biobricks.''' | ||
+ | *'''Site directed mutagenesis of phzM completed.''' | ||
+ | | | ||
+ | *'''Site directed mutagenesis of phzS completed.''' | ||
+ | |||
+ | |||
+ | |- align="center" | ||
+ | !width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week9 <font face=georgia color=#3366CC size=7><b>Week 9</b></font>] <br> | ||
+ | |||
+ | !width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week10 <font face=georgia color=#3366CC size=7><b>Week 10+</b></font>] <br> | ||
+ | |||
+ | |- valign=top | ||
+ | | | ||
+ | *'''phzD→phzG successfully cloned into TOPO vector.''' | ||
+ | | | ||
+ | *'''Contains further work achieved.''' | ||
+ | *'''XylR and Pr cloned into construction vectors.''' | ||
+ | *'''XylR and Pr put next to responsive promoter and luciferase.''' | ||
|} | |} | ||
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{|cellspacing="6px" cellpadding="16" border="0" width="100%" | {|cellspacing="6px" cellpadding="16" border="0" width="100%" | ||
- | |- | + | |- align=center |
- | |[ | + | |[https://2007.igem.org/Glasgow/Wetlab/Protocols <font face=georgia color=#3366CC size=5><b>Protocols</b></font>] |
- | |[ | + | |[https://2007.igem.org/Glasgow/Wetlab/References <font face=georgia color=#3366CC size=5><b>References</b></font>] |
- | |[ | + | |[https://2007.igem.org/Glasgow/Wetlab/Resources <font face=georgia color=#3366CC size=5><b>Resources</b></font>] |
- | |[ | + | |[https://2007.igem.org/Glasgow/Wetlab/Orders <font face=georgia color=#3366CC size=5><b>Orders</b></font>] |
- | |[ | + | |[https://2007.igem.org/Glasgow/Wetlab/Biobricks <font face=georgia color=#3366CC size=5><b>Biobricks<br>Used</b></font>] |
- | + | |[https://2007.igem.org/Glasgow/Wetlab/Gels <font face=georgia color=#3366CC size=5><b>Gels</b></font>] | |
|} | |} |
Latest revision as of 11:54, 26 October 2007
![]() | Back To Glasgow's Main Page | Go To Glasgow's Modelling Page |
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Protocols | References | Resources | Sequences | Biobricks Used | Gels |
Click on a week number for more detailed lab book...
Week 1 | Week 2 |
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Week 3 | Week 4 |
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Week 5 | Week 6 |
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Week 7 | Week 8 |
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Week 9 | Week 10+ |
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Protocols | References | Resources | Orders | Biobricks Used | Gels |