Week 14

From 2007.igem.org

(Difference between revisions)
 
(26 intermediate revisions not shown)
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::'''10/01/07'''
::'''10/01/07'''
-
 
+
*Meeting: Ethical issue.
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+
*Work on image segmentation algorithm.
::'''10/02/07'''
::'''10/02/07'''
 +
*Digestion of [http://partsregistry.org/Part:pSB4A3 pSB4A3];
 +
*Run on electrophoresis gel.
 +
*[http://partsregistry.org/Part:pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763033 I763033] band extraction.
 +
*Ligations for:
 +
::-[http://partsregistry.org/Part:BBa_I763004 I763004]+[http://partsregistry.org/Part:BBa_I763033 I763033];
 +
::-[http://partsregistry.org/Part:BBa_I763012 I763012]+[http://partsregistry.org/Part:BBa_I763020 I763020][http://partsregistry.org/Part:BBa_I763032 (I763032)].
Line 10: Line 16:
::'''10/03/07'''
::'''10/03/07'''
 +
*Digestions of:
 +
::-[http://partsregistry.org/Part:BBa_I763032 I763032] with Eco/Pst1;
 +
::-[http://partsregistry.org/Part:BBa_I763031 I763031] with Eco/Pst1;
 +
::-[http://partsregistry.org/Part:BBa_I763036 I763036] with Eco/Pst1;
 +
::-[http://partsregistry.org/Part:pSB4A3 pSB4A3] with Eco/Pst1.
 +
*Run on electrophoresis gel.
 +
*[http://partsregistry.org/Part:BBa_I763031 I763031], [http://partsregistry.org/Part:BBa_I763036 I763036] and [http://partsregistry.org/Part:pSB4A3 pSB4A3] band extraction.
 +
*Transformations of:
 +
::-[http://partsregistry.org/Part:BBa_I763034 I763034];
 +
::-[http://partsregistry.org/Part:BBa_I763031 I763031];
 +
::-[http://partsregistry.org/Part:BBa_I763032 I763032].
 +
*Ligations for:
 +
::-[http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
 +
::-[http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
 +
'''Experimental Setup'''
 +
 +
*To have an higher precision for our measurements, we have decided to get, for the same field of view on the same sample of bacteria solution, either a short film segment (from which we can extract frames) and a Microsoft Excel file, which contains a ten seconds interval of values from the photomultiplier output, taken at a 100Hz frequency.
 +
 +
*In order to have the same focus for the microscope's oculars, the photocamera and the photomultiplier we have to calibrate our system by changing the position of both camera and photomultiplier.
 +
 +
*Finally, we check that the photomultiplier field of view is the same size as the microscope's one: by moving the appropiate diaphragms, we adjust the previously done settings.
::'''10/04/07'''
::'''10/04/07'''
 +
*Trasformation of:
 +
::-[http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
 +
::-[http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
 +
*We inoculate [http://partsregistry.org/Part:BBa_I763034 I763034], [http://partsregistry.org/Part:BBa_I763031 I763031] + [http://partsregistry.org/Part:pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3] (in 6 ml).
Line 20: Line 51:
      
      
::'''10/05/07'''
::'''10/05/07'''
 +
*Stock preparation (500 ul) of [http://partsregistry.org/Part:I763034 I763034],[http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part:BBa_pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:BBa_pSB4A3 pSB4A3].
 +
*Miniprep of [http://partsregistry.org/Part:BBa_I763034 I763034], [http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part:BBa_pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:BBa_pSB4A3 pSB4A3].
 +
*Control digestion of:
 +
::-[http://partsregistry.org/Part:BBa_I763034 I763034] with Xba/Pst1;
 +
::-[http://partsregistry.org/Part:BBa_I763031 I763031] with Eco/Xba and Xba/Pst1;
 +
::-[http://partsregistry.org/Part:BBa_I763032 I763032] with Eco/Xba and Xba/Pst1.
 +
*Run on electrophoresis gel and [http://partsregistry.org/Part:BBa_I763032 I763032] (Eco/Xba and Xba/Pst1) band extraction.
-
 
+
[[Bologna#Diary | Back]]
-
[[Bologna | Back]]
+

Latest revision as of 15:51, 26 October 2007

10/01/07
  • Meeting: Ethical issue.
  • Work on image segmentation algorithm.


10/02/07
  • Digestion of [http://partsregistry.org/Part:pSB4A3 pSB4A3];
  • Run on electrophoresis gel.
  • [http://partsregistry.org/Part:pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763033 I763033] band extraction.
  • Ligations for:
-[http://partsregistry.org/Part:BBa_I763004 I763004]+[http://partsregistry.org/Part:BBa_I763033 I763033];
-[http://partsregistry.org/Part:BBa_I763012 I763012]+[http://partsregistry.org/Part:BBa_I763020 I763020][http://partsregistry.org/Part:BBa_I763032 (I763032)].



10/03/07
  • Digestions of:
-[http://partsregistry.org/Part:BBa_I763032 I763032] with Eco/Pst1;
-[http://partsregistry.org/Part:BBa_I763031 I763031] with Eco/Pst1;
-[http://partsregistry.org/Part:BBa_I763036 I763036] with Eco/Pst1;
-[http://partsregistry.org/Part:pSB4A3 pSB4A3] with Eco/Pst1.
  • Run on electrophoresis gel.
  • [http://partsregistry.org/Part:BBa_I763031 I763031], [http://partsregistry.org/Part:BBa_I763036 I763036] and [http://partsregistry.org/Part:pSB4A3 pSB4A3] band extraction.
  • Transformations of:
-[http://partsregistry.org/Part:BBa_I763034 I763034];
-[http://partsregistry.org/Part:BBa_I763031 I763031];
-[http://partsregistry.org/Part:BBa_I763032 I763032].
  • Ligations for:
-[http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
-[http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];

Experimental Setup

  • To have an higher precision for our measurements, we have decided to get, for the same field of view on the same sample of bacteria solution, either a short film segment (from which we can extract frames) and a Microsoft Excel file, which contains a ten seconds interval of values from the photomultiplier output, taken at a 100Hz frequency.
  • In order to have the same focus for the microscope's oculars, the photocamera and the photomultiplier we have to calibrate our system by changing the position of both camera and photomultiplier.
  • Finally, we check that the photomultiplier field of view is the same size as the microscope's one: by moving the appropiate diaphragms, we adjust the previously done settings.


10/04/07
  • Trasformation of:
-[http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
-[http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
  • We inoculate [http://partsregistry.org/Part:BBa_I763034 I763034], [http://partsregistry.org/Part:BBa_I763031 I763031] + [http://partsregistry.org/Part:pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3] (in 6 ml).



10/05/07
  • Stock preparation (500 ul) of [http://partsregistry.org/Part:I763034 I763034],[http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part:BBa_pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:BBa_pSB4A3 pSB4A3].
  • Miniprep of [http://partsregistry.org/Part:BBa_I763034 I763034], [http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part:BBa_pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:BBa_pSB4A3 pSB4A3].
  • Control digestion of:
-[http://partsregistry.org/Part:BBa_I763034 I763034] with Xba/Pst1;
-[http://partsregistry.org/Part:BBa_I763031 I763031] with Eco/Xba and Xba/Pst1;
-[http://partsregistry.org/Part:BBa_I763032 I763032] with Eco/Xba and Xba/Pst1.
  • Run on electrophoresis gel and [http://partsregistry.org/Part:BBa_I763032 I763032] (Eco/Xba and Xba/Pst1) band extraction.


Back

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