Glasgow/Wetlab

From 2007.igem.org

< Glasgow(Difference between revisions)
 
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!align=center|[https://2007.igem.org/Glasgow https://static.igem.org/mediawiki/2007/thumb/c/cc/Uog.jpg/50px-Uog.jpg] ||  [[Glasgow|<font face=georgia color=#3366CC size=4>Back To <br> Glasgow's <br> Main Page</font>]] || [[Glasgow/Wetlab|<font face=georgia color=#99CCFF size=4>Back To <br> Glasgow's <br> Drylab's Log</font>]]
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!align=center|[[Image:Uog.jpg]] ||    ||  [[Glasgow|<font face=georgia color=#3366CC size=4>Back To <br> Glasgow's <br> Main Page</font>]] || [[Glasgow/Modeling|<font face=georgia color=#3366CC size=4>Go To <br> Glasgow's <br> Modelling Page</font>]]
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{|cellspacing="6px" cellpadding="16" border="0" width="100%"
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|[https://2007.igem.org/Glasgow/Wetlab/Protocols <font face=georgia color=#99CCFF size=5><b>Protocols</b></font>]
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|[https://2007.igem.org/Glasgow/Wetlab/Protocols <font face=georgia color=#3366CC size=5><b>Protocols</b></font>]
|[https://2007.igem.org/Glasgow/Wetlab/References <font face=georgia color=#3366CC size=5><b>References</b></font>]
|[https://2007.igem.org/Glasgow/Wetlab/References <font face=georgia color=#3366CC size=5><b>References</b></font>]
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|[https://2007.igem.org/Glasgow/Wetlab/Resources <font face=georgia color=#99CCFF size=5><b>Resources</b></font>]
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|[https://2007.igem.org/Glasgow/Wetlab/Resources <font face=georgia color=#3366CC size=5><b>Resources</b></font>]
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|[https://2007.igem.org/Glasgow/Wetlab/Orders <font face=georgia color=#3366CC size=5><b>Orders</b></font>]
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|[https://2007.igem.org/Glasgow/Wetlab/Orders <font face=georgia color=#3366CC size=5><b>Sequences</b></font>]
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|[https://2007.igem.org/Glasgow/Wetlab/Biobricks <font face=georgia color=#99CCFF size=5><b>Biobricks<br>Used</b></font>]
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|[https://2007.igem.org/Glasgow/Wetlab/Biobricks <font face=georgia color=#3366CC size=5><b>Biobricks<br>Used</b></font>]
|[https://2007.igem.org/Glasgow/Wetlab/Gels <font face=georgia color=#3366CC size=5><b>Gels</b></font>]
|[https://2007.igem.org/Glasgow/Wetlab/Gels <font face=georgia color=#3366CC size=5><b>Gels</b></font>]
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*'''''First selection of biobricks transformed.'''
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*'''First selection of biobricks transformed.'''
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*'''''Preparation of stocks.'''
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*'''Preparation of stocks.'''
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*'''''Change of plan because DmpR failed to grow. '''
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*'''Researched DmpR, XylR and DntR sensor systems.'''
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*'''''Researched XylR and DntR sensor systems.'''
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*'''Designed primers for DmpR, DntR, XylR and associated promoters.'''
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*'''''Designed primers for DmpR, DntR, XylR and associated promoters.'''
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!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week3 <font face=georgia color=#99CCFF size=7><b>Week 3</b></font>] <br>  
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!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week3 <font face=georgia color=#3366CC size=7><b>Week 3</b></font>] <br>  
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!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week4 <font face=georgia color=#99CCFF size=7><b>Week 4</b></font>] <br>  
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!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week4 <font face=georgia color=#3366CC size=7><b>Week 4</b></font>] <br>  
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*'''PCR with new primers associated with DntR and XylR.'''
*'''PCR with new primers associated with DntR and XylR.'''
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* '''*m and *s cloned into TOPO vectors.'''
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* '''phzM and phzS cloned into TOPO vectors.'''
*'''Primer design for 7 gene operon.'''
*'''Primer design for 7 gene operon.'''
*'''DntR cloned into TOPO vector.'''
*'''DntR cloned into TOPO vector.'''
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*'''Suspected amplification of 7 gene operon.'''
*'''Suspected amplification of 7 gene operon.'''
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* '''*m and *s confirmed as present in TOPO vectors.'''
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* '''phzM and phzS confirmed as present in TOPO vectors.'''
*'''Confirmed presence of death gene plasmid in some vectors.'''
*'''Confirmed presence of death gene plasmid in some vectors.'''
*'''Miller assay performed with DntR system.'''
*'''Miller assay performed with DntR system.'''
*'''Yeast cells.'''
*'''Yeast cells.'''
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*'''Biobricks made of (*m), (*s), and DntR.'''
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*'''Biobricks made of phzM, phzS, and DntR.'''
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!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week7 <font face=georgia color=#99CCFF size=7><b>Week 7</b></font>] <br>  
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!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week7 <font face=georgia color=#3366CC size=7><b>Week 7</b></font>] <br>  
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!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week8 <font face=georgia color=#99CCFF size=7><b>Week 8</b></font>] <br>  
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!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week8 <font face=georgia color=#3366CC size=7><b>Week 8</b></font>] <br>  
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*'''Utilised new biobricks.'''
*'''Utilised new biobricks.'''
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*'''Site directed mutagenesis of (*m) completed.'''
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*'''Site directed mutagenesis of phzM completed.'''
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*'''Site directed mutagenesis of (*s) completed.'''
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*'''Site directed mutagenesis of phzS completed.'''
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*'''(*d→g*) successfully cloned into TOPO vector.'''
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!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week9 <font face=georgia color=#3366CC size=7><b>Week 9</b></font>] <br>  
!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week9 <font face=georgia color=#3366CC size=7><b>Week 9</b></font>] <br>  
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!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week10 <font face=georgia color=#3366CC size=7><b>Week 10</b></font>] <br>  
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!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week10 <font face=georgia color=#3366CC size=7><b>Week 10+</b></font>] <br>  
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*'''Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.'''
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*'''phzD→phzG successfully cloned into TOPO vector.'''
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*'''''Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.'''''
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*'''Contains further work achieved.'''
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*'''XylR and Pr cloned into construction vectors.'''
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*'''XylR and Pr put next to responsive promoter and luciferase.'''
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{|cellspacing="6px" cellpadding="16" border="0" width="100%"
{|cellspacing="6px" cellpadding="16" border="0" width="100%"
|- align=center
|- align=center
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|[https://2007.igem.org/Glasgow/Wetlab/Protocols <font face=georgia color=#99CCFF size=5><b>Protocols</b></font>]
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|[https://2007.igem.org/Glasgow/Wetlab/Protocols <font face=georgia color=#3366CC size=5><b>Protocols</b></font>]
|[https://2007.igem.org/Glasgow/Wetlab/References <font face=georgia color=#3366CC size=5><b>References</b></font>]
|[https://2007.igem.org/Glasgow/Wetlab/References <font face=georgia color=#3366CC size=5><b>References</b></font>]
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|[https://2007.igem.org/Glasgow/Wetlab/Resources <font face=georgia color=#99CCFF size=5><b>Resources</b></font>]
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|[https://2007.igem.org/Glasgow/Wetlab/Resources <font face=georgia color=#3366CC size=5><b>Resources</b></font>]
|[https://2007.igem.org/Glasgow/Wetlab/Orders <font face=georgia color=#3366CC size=5><b>Orders</b></font>]
|[https://2007.igem.org/Glasgow/Wetlab/Orders <font face=georgia color=#3366CC size=5><b>Orders</b></font>]
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|[https://2007.igem.org/Glasgow/Wetlab/Biobricks <font face=georgia color=#99CCFF size=5><b>Biobricks<br>Used</b></font>]
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|[https://2007.igem.org/Glasgow/Wetlab/Biobricks <font face=georgia color=#3366CC size=5><b>Biobricks<br>Used</b></font>]
|[https://2007.igem.org/Glasgow/Wetlab/Gels <font face=georgia color=#3366CC size=5><b>Gels</b></font>]
|[https://2007.igem.org/Glasgow/Wetlab/Gels <font face=georgia color=#3366CC size=5><b>Gels</b></font>]
|}
|}

Latest revision as of 11:54, 26 October 2007

Uog.jpg Back To
Glasgow's
Main Page 		Go To
Glasgow's
Modelling Page
Protocols References Resources Sequences Biobricks
Used 		Gels

Click on a week number for more detailed lab book...


Week 1
Week 2
  • First selection of biobricks transformed.
  • Preparation of stocks.
  • Researched DmpR, XylR and DntR sensor systems.
  • Designed primers for DmpR, DntR, XylR and associated promoters.
Week 3
Week 4
  • BioBricks tested by digestion and PCR.
  • PCR with new primers associated with DntR and XylR.
  • phzM and phzS cloned into TOPO vectors.
  • Primer design for 7 gene operon.
  • DntR cloned into TOPO vector.
  • Primer design for XylR and associated genes.
  • Death gene cloned into TOPO vector.
  • Primer design for death gene.
Week 5
Week 6
  • Suspected amplification of 7 gene operon.
  • phzM and phzS confirmed as present in TOPO vectors.
  • Confirmed presence of death gene plasmid in some vectors.
  • Miller assay performed with DntR system.
  • Yeast cells.
  • Biobricks made of phzM, phzS, and DntR.
Week 7
Week 8
  • Utilised new biobricks.
  • Site directed mutagenesis of phzM completed.
  • Site directed mutagenesis of phzS completed.


Week 9
Week 10+
  • phzD→phzG successfully cloned into TOPO vector.
  • Contains further work achieved.
  • XylR and Pr cloned into construction vectors.
  • XylR and Pr put next to responsive promoter and luciferase.
Protocols References Resources Orders Biobricks
Used 		Gels
Retrieved from "http://2007.igem.org/wiki/index.php/Glasgow/Wetlab"