Glasgow/Wetlab
From 2007.igem.org
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- | !align=center|[ | + | !align=center|[[Image:Uog.jpg]] || || [[Glasgow|<font face=georgia color=#3366CC size=4>Back To <br> Glasgow's <br> Main Page</font>]] || [[Glasgow/Modeling|<font face=georgia color=#3366CC size=4>Go To <br> Glasgow's <br> Modelling Page</font>]] |
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{|cellspacing="6px" cellpadding="16" border="0" width="100%" | {|cellspacing="6px" cellpadding="16" border="0" width="100%" | ||
|- align=center | |- align=center | ||
- | |[https://2007.igem.org/Glasgow/Wetlab/Protocols <font face=georgia color=# | + | |[https://2007.igem.org/Glasgow/Wetlab/Protocols <font face=georgia color=#3366CC size=5><b>Protocols</b></font>] |
|[https://2007.igem.org/Glasgow/Wetlab/References <font face=georgia color=#3366CC size=5><b>References</b></font>] | |[https://2007.igem.org/Glasgow/Wetlab/References <font face=georgia color=#3366CC size=5><b>References</b></font>] | ||
- | |[https://2007.igem.org/Glasgow/Wetlab/Resources <font face=georgia color=# | + | |[https://2007.igem.org/Glasgow/Wetlab/Resources <font face=georgia color=#3366CC size=5><b>Resources</b></font>] |
- | |[https://2007.igem.org/Glasgow/Wetlab/Orders <font face=georgia color=#3366CC size=5><b> | + | |[https://2007.igem.org/Glasgow/Wetlab/Orders <font face=georgia color=#3366CC size=5><b>Sequences</b></font>] |
- | |[https://2007.igem.org/Glasgow/Wetlab/Biobricks <font face=georgia color=# | + | |[https://2007.igem.org/Glasgow/Wetlab/Biobricks <font face=georgia color=#3366CC size=5><b>Biobricks<br>Used</b></font>] |
|[https://2007.igem.org/Glasgow/Wetlab/Gels <font face=georgia color=#3366CC size=5><b>Gels</b></font>] | |[https://2007.igem.org/Glasgow/Wetlab/Gels <font face=georgia color=#3366CC size=5><b>Gels</b></font>] | ||
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- | * | + | *'''First selection of biobricks transformed.''' |
- | * | + | *'''Preparation of stocks.''' |
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- | *''' | + | *'''Researched DmpR, XylR and DntR sensor systems.''' |
- | + | *'''Designed primers for DmpR, DntR, XylR and associated promoters.''' | |
- | * | + | |
|- align="center" | |- align="center" | ||
- | !width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week3 <font face=georgia color=# | + | !width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week3 <font face=georgia color=#3366CC size=7><b>Week 3</b></font>] <br> |
- | !width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week4 <font face=georgia color=# | + | !width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week4 <font face=georgia color=#3366CC size=7><b>Week 4</b></font>] <br> |
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*'''PCR with new primers associated with DntR and XylR.''' | *'''PCR with new primers associated with DntR and XylR.''' | ||
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- | * ''' | + | * '''phzM and phzS cloned into TOPO vectors.''' |
*'''Primer design for 7 gene operon.''' | *'''Primer design for 7 gene operon.''' | ||
*'''DntR cloned into TOPO vector.''' | *'''DntR cloned into TOPO vector.''' | ||
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*'''Suspected amplification of 7 gene operon.''' | *'''Suspected amplification of 7 gene operon.''' | ||
- | * ''' | + | * '''phzM and phzS confirmed as present in TOPO vectors.''' |
*'''Confirmed presence of death gene plasmid in some vectors.''' | *'''Confirmed presence of death gene plasmid in some vectors.''' | ||
*'''Miller assay performed with DntR system.''' | *'''Miller assay performed with DntR system.''' | ||
*'''Yeast cells.''' | *'''Yeast cells.''' | ||
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- | *'''Biobricks made of | + | *'''Biobricks made of phzM, phzS, and DntR.''' |
|- align="center" | |- align="center" | ||
- | !width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week7 <font face=georgia color=# | + | !width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week7 <font face=georgia color=#3366CC size=7><b>Week 7</b></font>] <br> |
- | !width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week8 <font face=georgia color=# | + | !width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week8 <font face=georgia color=#3366CC size=7><b>Week 8</b></font>] <br> |
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*'''Utilised new biobricks.''' | *'''Utilised new biobricks.''' | ||
- | *'''Site directed mutagenesis of | + | *'''Site directed mutagenesis of phzM completed.''' |
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- | *'''Site directed mutagenesis of | + | *'''Site directed mutagenesis of phzS completed.''' |
- | + | ||
|- align="center" | |- align="center" | ||
!width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week9 <font face=georgia color=#3366CC size=7><b>Week 9</b></font>] <br> | !width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week9 <font face=georgia color=#3366CC size=7><b>Week 9</b></font>] <br> | ||
- | !width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week10 <font face=georgia color=#3366CC size=7><b>Week 10</b></font>] <br> | + | !width="50%" |[https://2007.igem.org/Glasgow/Wetlab/Week10 <font face=georgia color=#3366CC size=7><b>Week 10+</b></font>] <br> |
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- | *''' | + | *'''phzD→phzG successfully cloned into TOPO vector.''' |
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- | *''''' | + | *'''Contains further work achieved.''' |
+ | *'''XylR and Pr cloned into construction vectors.''' | ||
+ | *'''XylR and Pr put next to responsive promoter and luciferase.''' | ||
|} | |} | ||
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{|cellspacing="6px" cellpadding="16" border="0" width="100%" | {|cellspacing="6px" cellpadding="16" border="0" width="100%" | ||
|- align=center | |- align=center | ||
- | |[https://2007.igem.org/Glasgow/Wetlab/Protocols <font face=georgia color=# | + | |[https://2007.igem.org/Glasgow/Wetlab/Protocols <font face=georgia color=#3366CC size=5><b>Protocols</b></font>] |
|[https://2007.igem.org/Glasgow/Wetlab/References <font face=georgia color=#3366CC size=5><b>References</b></font>] | |[https://2007.igem.org/Glasgow/Wetlab/References <font face=georgia color=#3366CC size=5><b>References</b></font>] | ||
- | |[https://2007.igem.org/Glasgow/Wetlab/Resources <font face=georgia color=# | + | |[https://2007.igem.org/Glasgow/Wetlab/Resources <font face=georgia color=#3366CC size=5><b>Resources</b></font>] |
|[https://2007.igem.org/Glasgow/Wetlab/Orders <font face=georgia color=#3366CC size=5><b>Orders</b></font>] | |[https://2007.igem.org/Glasgow/Wetlab/Orders <font face=georgia color=#3366CC size=5><b>Orders</b></font>] | ||
- | |[https://2007.igem.org/Glasgow/Wetlab/Biobricks <font face=georgia color=# | + | |[https://2007.igem.org/Glasgow/Wetlab/Biobricks <font face=georgia color=#3366CC size=5><b>Biobricks<br>Used</b></font>] |
|[https://2007.igem.org/Glasgow/Wetlab/Gels <font face=georgia color=#3366CC size=5><b>Gels</b></font>] | |[https://2007.igem.org/Glasgow/Wetlab/Gels <font face=georgia color=#3366CC size=5><b>Gels</b></font>] | ||
|} | |} |
Latest revision as of 11:54, 26 October 2007
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