Melbourne/Primary Reagents & disposables
From 2007.igem.org
< Melbourne(Difference between revisions)
| (5 intermediate revisions not shown) | |||
| Line 21: | Line 21: | ||
*[[Melbourne/primary DNA marker|DNA ladder]] | *[[Melbourne/primary DNA marker|DNA ladder]] | ||
*[[Melbourne/primary phosphatase|phospatase]] | *[[Melbourne/primary phosphatase|phospatase]] | ||
| + | *[[Melbourne/primary BSA|BSA]] | ||
| + | *[[Melbourne/primary GoTaq|GoTaq]] | ||
| + | *[[Melbourne/primary vf2|vf2 oligonucleotide]] | ||
| + | *[[Melbourne/primary vR|vR oligonucleotide]] | ||
===Antibiotics=== | ===Antibiotics=== | ||
*[[Melbourne/primary AMP|Ampicilian]] | *[[Melbourne/primary AMP|Ampicilian]] | ||
*[[Melbourne/primary Kan|Kanamycin]] | *[[Melbourne/primary Kan|Kanamycin]] | ||
| + | *[[Melbourne/primary Chloroamphenicol|Chloroamphenicol]] | ||
| + | *[[Melbourne/primary Gen|Gentimycin]] | ||
| + | |||
| + | |||
===Chemicals=== | ===Chemicals=== | ||
*[[Melbourne/primary agar|Agar]] | *[[Melbourne/primary agar|Agar]] | ||
Latest revision as of 11:48, 8 October 2007
<Back to team home page> <Template>
Contents |
Primary Reagents
Kits
- IGEM 2007 kit
- Miniprep kit
- Gel clean up kit
- Site directed mutagenesis (stratagene)
- Quickchange multi 200515(x10),200514(x30): 3-5 changes 1 primer each 50% <8Kb
- Quickchange 200518(x30) 200519(x10): single change 2 primers 90% <8Kb
- QuickchangeII XL 200522(x30) 200521(x10): single change 2 primers 80% >8Kb
- Primer design program : http://www.stratagene.com/sdmdesigner/default.aspx
Cells
Enzymes
- Melbourne/primary Restriction enzymes
- T4 Ligase
- T4 Ligase buffer 10X
- T4 Ligase buffer 2X
- DNA ladder
- phospatase
- BSA
- GoTaq
- vf2 oligonucleotide
- vR oligonucleotide
Antibiotics
Chemicals
- Agar
- Agarose
- Ethidium Bromide
- EDTA
- Glycerol
- Loading dye for DNA gel
- NaCl
- TRIS
- IPTG
- Xgal
- Bacto-tryptone
- Yeast Extract
- NaOH
- NZ amine
- Bacto-yeast Extract
- Ethanol
Other
Disposables
- Parrafilm
- Tips (10, 200, 1000 ul)
- Gloves (Large , Med, Small)
- Scalpels
- Tube racks
- Freezer boxes
