From 2007.igem.org

Revision as of 13:52, 3 October 2007 (view source) Guido Costa (Talk | contribs) ← Older edit		Latest revision as of 15:51, 26 October 2007 (view source) Francesca.bugane2 (Talk | contribs)
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	::'''10/01/07'''		::'''10/01/07'''
		+	*Meeting: Ethical issue.
		+	*Work on image segmentation algorithm.
		+	::'''10/02/07'''
		+	*Digestion of [http://partsregistry.org/Part:pSB4A3 pSB4A3];
		+	*Run on electrophoresis gel.
		+	*[http://partsregistry.org/Part:pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763033 I763033] band extraction.
		+	*Ligations for:
		+	::-[http://partsregistry.org/Part:BBa_I763004 I763004]+[http://partsregistry.org/Part:BBa_I763033 I763033];
		+	::-[http://partsregistry.org/Part:BBa_I763012 I763012]+[http://partsregistry.org/Part:BBa_I763020 I763020][http://partsregistry.org/Part:BBa_I763032 (I763032)].
-	::'''10/02/07'''
-	'''Experimental Setup'''
-	To have an higher precision for our measurements, we have decided to get, for the same field of view on the same sample of bacteria solution, either a short film segment (from which we can extract frames) and a Microsoft Excel file, which contains a ten seconds interval of values from the photomultiplier output, taken at a 100Hz frequency.	+	::'''10/03/07'''
		+	*Digestions of:
		+	::-[http://partsregistry.org/Part:BBa_I763032 I763032] with Eco/Pst1;
		+	::-[http://partsregistry.org/Part:BBa_I763031 I763031] with Eco/Pst1;
		+	::-[http://partsregistry.org/Part:BBa_I763036 I763036] with Eco/Pst1;
		+	::-[http://partsregistry.org/Part:pSB4A3 pSB4A3] with Eco/Pst1.
		+	*Run on electrophoresis gel.
		+	*[http://partsregistry.org/Part:BBa_I763031 I763031], [http://partsregistry.org/Part:BBa_I763036 I763036] and [http://partsregistry.org/Part:pSB4A3 pSB4A3] band extraction.
		+	*Transformations of:
		+	::-[http://partsregistry.org/Part:BBa_I763034 I763034];
		+	::-[http://partsregistry.org/Part:BBa_I763031 I763031];
		+	::-[http://partsregistry.org/Part:BBa_I763032 I763032].
		+	*Ligations for:
		+	::-[http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
		+	::-[http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
-	In order to have the same focus for the microscope's oculars, the photocamera and the photomultiplier we have to calibrate our system by changing the position of both camera and photomultiplier.	+	'''Experimental Setup'''
-	Finally, we check that the photomultiplier field of view is the same size as the microscope's one: by moving the appropiate diaphragms, we adjust the previously done settings.	+	*To have an higher precision for our measurements, we have decided to get, for the same field of view on the same sample of bacteria solution, either a short film segment (from which we can extract frames) and a Microsoft Excel file, which contains a ten seconds interval of values from the photomultiplier output, taken at a 100Hz frequency.
		+	*In order to have the same focus for the microscope's oculars, the photocamera and the photomultiplier we have to calibrate our system by changing the position of both camera and photomultiplier.
-	::'''10/03/07'''	+	*Finally, we check that the photomultiplier field of view is the same size as the microscope's one: by moving the appropiate diaphragms, we adjust the previously done settings.
-		+
	::'''10/04/07'''		::'''10/04/07'''
		+	*Trasformation of:
		+	::-[http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
		+	::-[http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
		+	*We inoculate [http://partsregistry.org/Part:BBa_I763034 I763034], [http://partsregistry.org/Part:BBa_I763031 I763031] + [http://partsregistry.org/Part:pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3] (in 6 ml).
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	::'''10/05/07'''		::'''10/05/07'''
		+	*Stock preparation (500 ul) of [http://partsregistry.org/Part:I763034 I763034],[http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part:BBa_pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:BBa_pSB4A3 pSB4A3].
		+	*Miniprep of [http://partsregistry.org/Part:BBa_I763034 I763034], [http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part:BBa_pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:BBa_pSB4A3 pSB4A3].
		+	*Control digestion of:
		+	::-[http://partsregistry.org/Part:BBa_I763034 I763034] with Xba/Pst1;
		+	::-[http://partsregistry.org/Part:BBa_I763031 I763031] with Eco/Xba and Xba/Pst1;
		+	::-[http://partsregistry.org/Part:BBa_I763032 I763032] with Eco/Xba and Xba/Pst1.
		+	*Run on electrophoresis gel and [http://partsregistry.org/Part:BBa_I763032 I763032] (Eco/Xba and Xba/Pst1) band extraction.
-		+	[[Bologna#Diary | Back]]
-	[[Bologna | Back]]	+

---

Latest revision as of 15:51, 26 October 2007

10/01/07

• Meeting: Ethical issue.
• Work on image segmentation algorithm.

10/02/07

• Digestion of [http://partsregistry.org/Part:pSB4A3 pSB4A3];
• Run on electrophoresis gel.
• [http://partsregistry.org/Part:pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763033 I763033] band extraction.
• Ligations for:

-[http://partsregistry.org/Part:BBa_I763004 I763004]+[http://partsregistry.org/Part:BBa_I763033 I763033];

-[http://partsregistry.org/Part:BBa_I763012 I763012]+[http://partsregistry.org/Part:BBa_I763020 I763020][http://partsregistry.org/Part:BBa_I763032 (I763032)].

10/03/07

• Digestions of:

-[http://partsregistry.org/Part:BBa_I763032 I763032] with Eco/Pst1;

-[http://partsregistry.org/Part:BBa_I763031 I763031] with Eco/Pst1;

-[http://partsregistry.org/Part:BBa_I763036 I763036] with Eco/Pst1;

-[http://partsregistry.org/Part:pSB4A3 pSB4A3] with Eco/Pst1.

• Run on electrophoresis gel.
• [http://partsregistry.org/Part:BBa_I763031 I763031], [http://partsregistry.org/Part:BBa_I763036 I763036] and [http://partsregistry.org/Part:pSB4A3 pSB4A3] band extraction.
• Transformations of:

-[http://partsregistry.org/Part:BBa_I763034 I763034];

-[http://partsregistry.org/Part:BBa_I763031 I763031];

-[http://partsregistry.org/Part:BBa_I763032 I763032].

• Ligations for:

-[http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];

-[http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];

Experimental Setup

• To have an higher precision for our measurements, we have decided to get, for the same field of view on the same sample of bacteria solution, either a short film segment (from which we can extract frames) and a Microsoft Excel file, which contains a ten seconds interval of values from the photomultiplier output, taken at a 100Hz frequency.

• In order to have the same focus for the microscope's oculars, the photocamera and the photomultiplier we have to calibrate our system by changing the position of both camera and photomultiplier.

• Finally, we check that the photomultiplier field of view is the same size as the microscope's one: by moving the appropiate diaphragms, we adjust the previously done settings.

10/04/07

• Trasformation of:

-[http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];

-[http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];

• We inoculate [http://partsregistry.org/Part:BBa_I763034 I763034], [http://partsregistry.org/Part:BBa_I763031 I763031] + [http://partsregistry.org/Part:pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3] (in 6 ml).

10/05/07

• Stock preparation (500 ul) of [http://partsregistry.org/Part:I763034 I763034],[http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part:BBa_pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:BBa_pSB4A3 pSB4A3].
• Miniprep of [http://partsregistry.org/Part:BBa_I763034 I763034], [http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part:BBa_pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:BBa_pSB4A3 pSB4A3].
• Control digestion of:

-[http://partsregistry.org/Part:BBa_I763034 I763034] with Xba/Pst1;

-[http://partsregistry.org/Part:BBa_I763031 I763031] with Eco/Xba and Xba/Pst1;

-[http://partsregistry.org/Part:BBa_I763032 I763032] with Eco/Xba and Xba/Pst1.

• Run on electrophoresis gel and [http://partsregistry.org/Part:BBa_I763032 I763032] (Eco/Xba and Xba/Pst1) band extraction.

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