Paris/October 17

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[[Paris/October 16|yesterday]] -- [[Paris/October 18|tomorrow]] <br>
[[Paris/October 16|yesterday]] -- [[Paris/October 18|tomorrow]] <br>
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{|
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|- style="background: #ccccff;"
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! colspan="5" style="background: #ccccff;" | Digestion Products
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|- style="background: #ccccff; text-align: center;"
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|width=5%| Number
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|width=24%| Product Name
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|width=24%| Matrix Name
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|width=5%| Enzyme 1
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|width=5%| Enzyme 2
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|width=5%| Size
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|width=34%| Description
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|width=1%|
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|width=1%|
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|width=1%|
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |D94
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|PacI[ptet>>lox71-gfpT-lox66-mrfp]SmaI
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|P15
 +
|PacI
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|XmaI
 +
|
 +
|ptet>>lox71-gfpT-lox66-mrfp P15 PCR product digested by PacI-SmaI for cloning into pY-2C-IntC
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|
 +
|
 +
|
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D95
 +
|PacI[pY-2C-IntC]SmaI
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|pY-2C-IntC plasmid
 +
|PacI
 +
|XmaI
 +
|
 +
|pY-2C-IntC digested for ptet>>lox71-gfpT-lox66-mrfp cloning
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|
 +
|
 +
|
 +
|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |D82
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|frt1-CmR-frt2 FI
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|P16
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|EcoRI
 +
|SpeI
 +
|
 +
|frt1-CmR-frt2 PCR product digested for use as a FI
 +
|
 +
|
 +
|
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D83
 +
|frt1-CmR-frt2 BI
 +
|P16
 +
|XbaI
 +
|PstI
 +
|
 +
|frt1-CmR-frt2 PCR product digested for use as a BI
 +
|
 +
|
 +
|
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D84
 +
|frt1-CmR-frt2 BBi
 +
|P16
 +
|EcoRI
 +
|PstI
 +
|
 +
|frt1-CmR-frt2 PCR product digested for cloning as a BB
 +
|
 +
|
 +
|
 +
|}
 +
 +
 +
Unfortunatly, D82 digestion reaction gave an unexpected result with a band at 500bp

Latest revision as of 03:40, 21 October 2007

yesterday -- tomorrow


Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 Size Description
D94 PacI[ptet>>lox71-gfpT-lox66-mrfp]SmaI P15 PacI XmaI ptet>>lox71-gfpT-lox66-mrfp P15 PCR product digested by PacI-SmaI for cloning into pY-2C-IntC
D95 PacI[pY-2C-IntC]SmaI pY-2C-IntC plasmid PacI XmaI pY-2C-IntC digested for ptet>>lox71-gfpT-lox66-mrfp cloning
D82 frt1-CmR-frt2 FI P16 EcoRI SpeI frt1-CmR-frt2 PCR product digested for use as a FI
D83 frt1-CmR-frt2 BI P16 XbaI PstI frt1-CmR-frt2 PCR product digested for use as a BI
D84 frt1-CmR-frt2 BBi P16 EcoRI PstI frt1-CmR-frt2 PCR product digested for cloning as a BB


Unfortunatly, D82 digestion reaction gave an unexpected result with a band at 500bp

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