Tokyo/Formulation/1.toggle model

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＜中身＞
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__NOTOC__
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（相平面解析）
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<br>[[Tokyo/Works|Works top]]&nbsp;&nbsp;&nbsp;0.[[Tokyo/Works/Hybrid promoter|Hybrid promoter]]&nbsp;&nbsp;&nbsp;'''1.[[Tokyo/Works/Formulation |Formulation]]'''&nbsp;&nbsp;&nbsp;2.[[Tokyo/Works/Assay |Assay1]]&nbsp;&nbsp;&nbsp;3.[[Tokyo/Works/Simulation |Simulation]]&nbsp;&nbsp;&nbsp;4.[[Tokyo/Works/Assay2 |Assay2]]&nbsp;&nbsp;&nbsp;5.[[Tokyo/Works/Future works |Future works]]
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パラメータによって相平面が変わり，平衡点が１つのときと３つのときがある．
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<br><br>[[Tokyo/Formulation/1.toggle model |Step1]]&nbsp;&nbsp;&nbsp;[[Tokyo/Formulation/2.toggle model with hybrid promoter |Step2]]&nbsp;&nbsp;&nbsp;[[Tokyo/Formulation/3.AHL-experssing model|Step3]]　　
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平衡点が３つのときbistableになり，A状態，B状態ができる．
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<br>
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（シミュレーション）
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== 1.Single cell model:mutual inhibition ==
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初期値を安定点付近から始めるとちゃんとそのまま安定してるよ
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First, the ordinary differential equations (ODEs) of the toggle switch were derived as
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<br>[[Image:expression1-1.jpg|200px|left|thumb|Ex1-1 ]]  [[Image:parameter1-1.jpg|200px|]]
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[[Image:式-1.JPG|200px|]]
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<br>These equations were normalized as follows:
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[[Image:toggle1.jpg|200px|]] [[Image:toggle2.jpg|200px|]]
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<br>[[Image:expression1-2.jpg|200px|none|thumb|Ex1-2 ]]
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<br>In the steady state, time derivatives are zero:
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<br>[[Image:expression1-3.jpg|80px|none|thumb|Ex1-3]]
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<br>As a result, the nullclines of this system were derived as
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<br>[[Image:Siki2.jpg|200px|none|thumb|Ex1-4]]
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<br>which indicate the nullclines of the system shown in Fig 1.1.A-C. Where about parameters, we use three sets of parameters.
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<br>　　A)the maximum expression rate of repressor A and repressor B is balanced,and hill coefficient of both A and B is three.
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<br>[[Image:parameter1-2.jpg|150px|center|thumb|Table1.A]]
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<br>　　B)the maximum expression rate of repressor A and repressor B is equal,and hill coefficient of A is one.
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<br>[[Image:parameter1-3.JPG|150px|center|thumb|Table1.B]]
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<br>　　C)the maximum expression rate of repressor A and repressor B is not balanced,and hill coefficient of both A and B is three.
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<br>[[Image:parameter1-4.JPG|150px|center|thumb|Table1.C]]
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[[Image:toggle1.jpg|260px|left|thumb|Figure 1.1.A]]
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[[Image:toggle2.jpg|270px|left|thumb|Figure 1.1.B]]
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[[Image:Toggle1-4.jpg|270px|none|thumb|Figure 1.1.C]]
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<br>First,we carried out kinetic simulations in the condition of Fig 1.1.A. The results are shown in Fig 1.2.A-C.
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[[Image:toggle3.jpg|200px|left|thumb|Figure 1.2.A  (Ra(0),Rb(0))=(0.0,2.5)]] [[Image:toggle4.JPG|200px|left|thumb|Figure 1.2.B  (Ra(0),Rb(0))=(2.5,0.0)]]
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[[Image:toggle5.JPG|200px|left|thumb|Figure 1.2.C  (Ra(0),Rb(0))=(1.5,1.3)]]
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[[Image:toggle1-1.jpg|200px|none|thumb|Figure 3  bistable]]
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<br>Fig 1.2.A-C indicate that when the initial condition is (Ra,Rb)=(0.0,2.5), which is near the stable equilibrium point B, the values of Ra and Rb go to stable equilibrium point B, and when the initial condition is (Ra,Rb)=(2.5,0.0), which is near the stable equilibrium point A, the values of Ra and Rb go to the stable equilibrium point B.
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<br>Next, when the number of stable equilibrium point is one(Fig 1.1.B), the result of simulation are shown in Fig 1.4.A-C.
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[[Image:toggle6.JPG|200px|left|thumb|Figure 1.4.A  (Ra(0),Rb(0))=(0.0,2.5)]] [[Image:toggle7.JPG|200px|left|thumb|Figure 1.4.B  (Ra(0),Rb(0))=(2.5,0.0)]]
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[[Image:toggle8.JPG|200px|left|thumb|Figure 1.4.C  (Ra(0),Rb(0))=(1.5,1.3)]]
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[[Image:toggle1-2.jpg|220px|none|thumb|Figure 1.5  monostable]]
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<br><br>
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<br>
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<br>Fig 4.A-C indicate that the value of Ra and Rb go to the stable equilibrium point A regardless of an initial value in case of monostable state.
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<br>'''As a result, taking two stable states needs the phaseplane of two stable equilibrium points and Hill coefficients was very important.'''
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== ==
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[[Tokyo/Formulation/1.toggle model|Step.1]] >> [[Tokyo/Formulation/2.toggle model with hybrid promoter|Step.2]]

Latest revision as of 02:45, 27 October 2007


Works top   0.Hybrid promoter   1.Formulation   2.Assay1   3.Simulation   4.Assay2   5.Future works

Step1   Step2   Step3　　

1.Single cell model:mutual inhibition

First, the ordinary differential equations (ODEs) of the toggle switch were derived as


Ex1-1
Parameter1-1.jpg


These equations were normalized as follows:


Ex1-2


In the steady state, time derivatives are zero:


Ex1-3


As a result, the nullclines of this system were derived as


Ex1-4


which indicate the nullclines of the system shown in Fig 1.1.A-C. Where about parameters, we use three sets of parameters.


　　A)the maximum expression rate of repressor A and repressor B is balanced,and hill coefficient of both A and B is three.


Table1.A


　　B)the maximum expression rate of repressor A and repressor B is equal,and hill coefficient of A is one.


Table1.B


　　C)the maximum expression rate of repressor A and repressor B is not balanced,and hill coefficient of both A and B is three.


Table1.C
Figure 1.1.A
Figure 1.1.B
Figure 1.1.C


First,we carried out kinetic simulations in the condition of Fig 1.1.A. The results are shown in Fig 1.2.A-C.

Figure 1.2.A (Ra(0),Rb(0))=(0.0,2.5)
Figure 1.2.B (Ra(0),Rb(0))=(2.5,0.0)
Figure 1.2.C (Ra(0),Rb(0))=(1.5,1.3)
Figure 3 bistable



Fig 1.2.A-C indicate that when the initial condition is (Ra,Rb)=(0.0,2.5), which is near the stable equilibrium point B, the values of Ra and Rb go to stable equilibrium point B, and when the initial condition is (Ra,Rb)=(2.5,0.0), which is near the stable equilibrium point A, the values of Ra and Rb go to the stable equilibrium point B.


Next, when the number of stable equilibrium point is one(Fig 1.1.B), the result of simulation are shown in Fig 1.4.A-C.

Figure 1.4.A (Ra(0),Rb(0))=(0.0,2.5)
Figure 1.4.B (Ra(0),Rb(0))=(2.5,0.0)
Figure 1.4.C (Ra(0),Rb(0))=(1.5,1.3)
Figure 1.5 monostable





Fig 4.A-C indicate that the value of Ra and Rb go to the stable equilibrium point A regardless of an initial value in case of monostable state.


As a result, taking two stable states needs the phaseplane of two stable equilibrium points and Hill coefficients was very important.

Step.1 >> Step.2

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