Paris/October 17
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| + | |- style="background: #cccccc;" | ||
| + | | style="background: #ccffcc;" |D94 | ||
| + | |PacI[ptet>>lox71-gfpT-lox66-mrfp]SmaI | ||
| + | |P15 | ||
| + | |PacI | ||
| + | |XmaI | ||
| + | | | ||
| + | |ptet>>lox71-gfpT-lox66-mrfp P15 PCR product digested by PacI-SmaI for cloning into pY-2C-IntC | ||
| + | | | ||
| + | | | ||
| + | | | ||
| + | |- style="background: #cccccc;" | ||
| + | | style="background: #ccffcc;" |D95 | ||
| + | |PacI[pY-2C-IntC]SmaI | ||
| + | |pY-2C-IntC plasmid | ||
| + | |PacI | ||
| + | |XmaI | ||
| + | | | ||
| + | |pY-2C-IntC digested for ptet>>lox71-gfpT-lox66-mrfp cloning | ||
| + | | | ||
| + | | | ||
| + | | | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D82 | | style="background: #ccffcc;" |D82 | ||
Latest revision as of 03:40, 21 October 2007
| Digestion Products | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Number | Product Name | Matrix Name | Enzyme 1 | Enzyme 2 | Size | Description | |||
| D94 | PacI[ptet>>lox71-gfpT-lox66-mrfp]SmaI | P15 | PacI | XmaI | ptet>>lox71-gfpT-lox66-mrfp P15 PCR product digested by PacI-SmaI for cloning into pY-2C-IntC | ||||
| D95 | PacI[pY-2C-IntC]SmaI | pY-2C-IntC plasmid | PacI | XmaI | pY-2C-IntC digested for ptet>>lox71-gfpT-lox66-mrfp cloning | ||||
| D82 | frt1-CmR-frt2 FI | P16 | EcoRI | SpeI | frt1-CmR-frt2 PCR product digested for use as a FI | ||||
| D83 | frt1-CmR-frt2 BI | P16 | XbaI | PstI | frt1-CmR-frt2 PCR product digested for use as a BI | ||||
| D84 | frt1-CmR-frt2 BBi | P16 | EcoRI | PstI | frt1-CmR-frt2 PCR product digested for cloning as a BB | ||||
Unfortunatly, D82 digestion reaction gave an unexpected result with a band at 500bp
