Glasgow/Wetlab/Gels

From 2007.igem.org

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|[[Glasgow/Wetlab/Week6#Friday 10th August 2007|10th Aug 2007 (2)]]
|[[Glasgow/Wetlab/Week6#Friday 10th August 2007|10th Aug 2007 (2)]]
|Colony PCR after ligating (*m*), (*s*), DntR and Pu with the construction vectors 3/20G and 4/6B. Gel 2.
|Colony PCR after ligating (*m*), (*s*), DntR and Pu with the construction vectors 3/20G and 4/6B. Gel 2.
-
|1.(*s*)<br>
+
|'''1.'''(*s*)<br>
-
2.(*s*)<br>
+
'''2.'''(*s*)<br>
-
3.neg control<br>
+
'''3.'''neg control<br>
-
4.DntR<br>
+
'''4.'''DntR<br>
-
5.DntR<br>
+
'''5.'''DntR<br>
-
6.Pu<br>
+
'''6.'''Pu<br>
-
7.Pu<br>
+
'''7.'''Pu<br>
-
8.neg control<br>
+
'''8.'''neg control<br>
-
9.neg control<br>
+
'''9.'''neg control<br>
-
10.neg control<br>
+
'''10.'''neg control<br>
-
11.neg control<br>
+
'''11.'''neg control<br>
|[[Image:10thAug gel 2.JPG|200px]]
|[[Image:10thAug gel 2.JPG|200px]]
|-
|-
|[[Glasgow/Wetlab/Week7#Monday 13th August 2007|13th Aug 2007 (4)]]
|[[Glasgow/Wetlab/Week7#Monday 13th August 2007|13th Aug 2007 (4)]]
|PCR of genes (*a→g*), (*a→d*), and (*d→g*).
|PCR of genes (*a→g*), (*a→d*), and (*d→g*).
-
|1A:(*a→g*) <br>
+
|'''1A.'''(*a→g*) <br>
-
1B:(*a→g*) <br>
+
'''1B.'''(*a→g*) <br>
-
1C:(*a→g*) <br>
+
'''1C.'''(*a→g*) <br>
-
2A:(*a→g*) <br>
+
'''2A.'''(*a→g*) <br>
-
2B:(*a→d*) <br>
+
'''2B.'''(*a→d*) <br>
-
2C:(*d→g*) <br>
+
'''2C.'''(*d→g*) <br>
|[[Image:2007-08-13 12hr 43min.JPG|200px]]
|[[Image:2007-08-13 12hr 43min.JPG|200px]]
|-
|-
|[[Glasgow/Wetlab/Week7#Friday 17th August 2007|17th Aug 2007 (2-4)]]
|[[Glasgow/Wetlab/Week7#Friday 17th August 2007|17th Aug 2007 (2-4)]]
|PstI digested minipreps of (*m*) and (*s*) in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis. Gel 1.
|PstI digested minipreps of (*m*) and (*s*) in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis. Gel 1.
-
|1.-3.(*m*)+4/6B<br>(PstI site mutated)<br>
+
|'''1.-3.'''(*m*)+4/6B<br>(PstI site mutated)<br>
-
4.-6.(*m*)+3/20G<br>(PstI site mutated)<br>
+
'''4.-6.'''(*m*)+3/20G<br>(PstI site mutated)<br>
-
7.-9.(*s*)+4/6B<br>(1st PstI site mutated)<br>
+
'''7.-9.'''(*s*)+4/6B<br>(1st PstI site mutated)<br>
-
10.-12.(*s*)+3/20G<br>(2nd PstI site mutated)<br>
+
'''10.-12.'''(*s*)+3/20G<br>(2nd PstI site mutated)<br>
-
13.-15.(*s*)+4/6B<br>(2nd PstI site mutated)<br>
+
'''13.-15.'''(*s*)+4/6B<br>(2nd PstI site mutated)<br>
-
16.(*s*)+3/20G<br>(1st PstI site mutated)<br>
+
'''16.'''(*s*)+3/20G<br>(1st PstI site mutated)<br>
|[[Image:17thAug SDM1 PstI digest gel 1.JPG|200px]]
|[[Image:17thAug SDM1 PstI digest gel 1.JPG|200px]]
|-
|-
|[[Glasgow/Wetlab/Week7#Friday 17th August 2007|17th Aug 2007 (2-4)]]
|[[Glasgow/Wetlab/Week7#Friday 17th August 2007|17th Aug 2007 (2-4)]]
|PstI digested minipreps of (*m*) and (*s*) in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis. Gel 2.
|PstI digested minipreps of (*m*) and (*s*) in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis. Gel 2.
-
|1.-2.(*s*)+3/20G<br>(1st PstI site mutated)<br>
+
|'''1.-2.'''(*s*)+3/20G<br>(1st PstI site mutated)<br>
-
3.(*m*)+4/6B<br>('''Before''' mutagenesis)<br>
+
'''3.'''(*m*)+4/6B<br>('''Before''' mutagenesis)<br>
-
4.(*m*)+3/20G<br>('''Before''' mutagenesis)<br>
+
'''4.'''(*m*)+3/20G<br>('''Before''' mutagenesis)<br>
-
5.(*s*)+4/6B<br>('''Before''' mutagenesis)<br>
+
'''5.'''(*s*)+4/6B<br>('''Before''' mutagenesis)<br>
-
6.(*s*)+3/20G<br>('''Before''' mutagenesis)<br>
+
'''6.'''(*s*)+3/20G<br>('''Before''' mutagenesis)<br>
|[[Image:17thAug SDM1 PstI digest gel 2.JPG|200px]]
|[[Image:17thAug SDM1 PstI digest gel 2.JPG|200px]]
|}
|}
<br>
<br>

Revision as of 14:48, 28 August 2007

Glasgow Main Page | Back To Wetlab Log

Gels

Gels are labelled with numbers for rows and letters for columns.

Date and Reference Description Key Gel
10th Aug 2007 (2) Colony PCR after ligating (*m*), (*s*), DntR and Pu with the construction vectors 3/20G and 4/6B. Gel 1. 1.(*m*)

2.(*m*)
3.(*s*)
4.(*s*)
5.(*s*)
6.(*s*)
7.neg control
8.neg control
9.neg control

10thAug gel 1.JPG
10th Aug 2007 (2) Colony PCR after ligating (*m*), (*s*), DntR and Pu with the construction vectors 3/20G and 4/6B. Gel 2. 1.(*s*)

2.(*s*)
3.neg control
4.DntR
5.DntR
6.Pu
7.Pu
8.neg control
9.neg control
10.neg control
11.neg control

10thAug gel 2.JPG
13th Aug 2007 (4) PCR of genes (*a→g*), (*a→d*), and (*d→g*). 1A.(*a→g*)

1B.(*a→g*)
1C.(*a→g*)
2A.(*a→g*)
2B.(*a→d*)
2C.(*d→g*)

2007-08-13 12hr 43min.JPG
17th Aug 2007 (2-4) PstI digested minipreps of (*m*) and (*s*) in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis. Gel 1. 1.-3.(*m*)+4/6B
(PstI site mutated)

4.-6.(*m*)+3/20G
(PstI site mutated)
7.-9.(*s*)+4/6B
(1st PstI site mutated)
10.-12.(*s*)+3/20G
(2nd PstI site mutated)
13.-15.(*s*)+4/6B
(2nd PstI site mutated)
16.(*s*)+3/20G
(1st PstI site mutated)

17thAug SDM1 PstI digest gel 1.JPG
17th Aug 2007 (2-4) PstI digested minipreps of (*m*) and (*s*) in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis. Gel 2. 1.-2.(*s*)+3/20G
(1st PstI site mutated)

3.(*m*)+4/6B
(Before mutagenesis)
4.(*m*)+3/20G
(Before mutagenesis)
5.(*s*)+4/6B
(Before mutagenesis)
6.(*s*)+3/20G
(Before mutagenesis)

17thAug SDM1 PstI digest gel 2.JPG


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