Glasgow/Wetlab/Gels
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|[[Glasgow/Wetlab/Week8#Thursday 23rd August 2007|23rd Aug 2007]] | |[[Glasgow/Wetlab/Week8#Thursday 23rd August 2007|23rd Aug 2007]] | ||
| - | |PstI | + | |PstI digested (*s*) minipreps in 4/6B and 3/20G vectors after the second round of site-directed mutagenesis. |
|'''1.-5.'''(*s*)+4/6B<br> | |'''1.-5.'''(*s*)+4/6B<br> | ||
'''6.-16.'''(*s*)+3/20G<br> | '''6.-16.'''(*s*)+3/20G<br> | ||
Revision as of 11:06, 29 August 2007
Glasgow Main Page | Back To Wetlab Log
Gels
Gels are labelled with numbers for rows and letters for columns.
| Date and Reference | Description | Key | Gel |
|---|---|---|---|
| 10th Aug 2007 (2) | Colony PCR after ligating (*m*), (*s*), DntR and Pu with the construction vectors 3/20G and 4/6B. Gel 1. | 1.(*m*) 2.(*m*) | 
|
| 10th Aug 2007 (2) | Colony PCR after ligating (*m*), (*s*), DntR and Pu with the construction vectors 3/20G and 4/6B. Gel 2. | 1.(*s*) 2.(*s*) | 
|
| 13th Aug 2007 (4) | PCR of genes (*a→g*), (*a→d*), and (*d→g*). | 1A.(*a→g*) 1B.(*a→g*) | 
|
| 17th Aug 2007 (2-4) | PstI digested minipreps of (*m*) and (*s*) in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis. Gel 1. | 1.-3.(*m*)+4/6B (PstI site mutated) 4.-6.(*m*)+3/20G | 
|
| 17th Aug 2007 (2-4) | PstI digested minipreps of (*m*) and (*s*) in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis. Gel 2. | 1.-2.(*s*)+3/20G (1st PstI site mutated) 3.(*m*)+4/6B | 
|
| 23rd Aug 2007 | (*s*) minipreps in 4/6B and 3/20G run after the second round of site-directed mutagenesis. | 1.-5.(*s*)+4/6B 6.-16.(*s*)+3/20G | 
|
| 23rd Aug 2007 | PstI digested (*s*) minipreps in 4/6B and 3/20G vectors after the second round of site-directed mutagenesis. | 1.-5.(*s*)+4/6B 6.-16.(*s*)+3/20G | 
|
| 23rd Aug 2007 | Colony PCR with "Pr_Prefix" and "XylR_Suffix" primers from Lynsey's transformations with Pr+XylR miniprep DNA. Gel 1. | 1.-7.colonies from the plate | 
|
| 23rd Aug 2007 | Colony PCR with "Pr_Prefix" and "XylR_Suffix" primers from Lynsey's transformations with Pr+XylR miniprep DNA. Gel 2. | 8.-12.colonies from the plate 13.neg control | 
|
