Glasgow/Wetlab/Gels
From 2007.igem.org
(Difference between revisions)
(→Gels) |
(→Gels) |
||
| Line 36: | Line 36: | ||
'''11.'''neg control<br> | '''11.'''neg control<br> | ||
|[[Image:10thAug gel 2.JPG|200px]] | |[[Image:10thAug gel 2.JPG|200px]] | ||
| + | |} | ||
| + | |||
| + | {| border="1" cellspacing="0" cellpadding="5" align="center" | ||
| + | !Date and Reference | ||
| + | !Description | ||
| + | !Key | ||
| + | !Gel | ||
|- | |- | ||
|[[Glasgow/Wetlab/Week7#Monday 13th August 2007|13th Aug 2007 (4)]] | |[[Glasgow/Wetlab/Week7#Monday 13th August 2007|13th Aug 2007 (4)]] | ||
| Line 46: | Line 53: | ||
'''2C.'''(*d→g*) <br> | '''2C.'''(*d→g*) <br> | ||
|[[Image:2007-08-13 12hr 43min.JPG|200px]] | |[[Image:2007-08-13 12hr 43min.JPG|200px]] | ||
| + | |} | ||
| + | |||
| + | {| border="1" cellspacing="0" cellpadding="5" align="center" | ||
| + | !Date and Reference | ||
| + | !Description | ||
| + | !Key | ||
| + | !Gel | ||
|- | |- | ||
|[[Glasgow/Wetlab/Week7#Friday 17th August 2007|17th Aug 2007 (2-4)]] | |[[Glasgow/Wetlab/Week7#Friday 17th August 2007|17th Aug 2007 (2-4)]] | ||
| Line 65: | Line 79: | ||
'''6.'''(*s*)+3/20G<br>('''Before''' mutagenesis)<br> | '''6.'''(*s*)+3/20G<br>('''Before''' mutagenesis)<br> | ||
|[[Image:17thAug SDM1 PstI digest gel 2.JPG|200px]] | |[[Image:17thAug SDM1 PstI digest gel 2.JPG|200px]] | ||
| + | |} | ||
| + | |||
| + | {| border="1" cellspacing="0" cellpadding="5" align="center" | ||
| + | !Date and Reference | ||
| + | !Description | ||
| + | !Key | ||
| + | !Gel | ||
|- | |- | ||
|[[Glasgow/Wetlab/Week8#Thursday 23rd August 2007|23rd Aug 2007]] | |[[Glasgow/Wetlab/Week8#Thursday 23rd August 2007|23rd Aug 2007]] | ||
Revision as of 11:29, 29 August 2007
Glasgow Main Page | Back To Wetlab Log
Gels
Gels are labelled with numbers for rows and letters for columns.
| Date and Reference | Description | Key | Gel |
|---|---|---|---|
| 10th Aug 2007 (2) | Colony PCR after ligating (*m*), (*s*), DntR and Pu with the construction vectors 3/20G and 4/6B. Gel 1. | 1.(*m*) 2.(*m*) | 
|
| 10th Aug 2007 (2) | Colony PCR after ligating (*m*), (*s*), DntR and Pu with the construction vectors 3/20G and 4/6B. Gel 2. | 1.(*s*) 2.(*s*) | 
|
| Date and Reference | Description | Key | Gel |
|---|---|---|---|
| 13th Aug 2007 (4) | PCR of genes (*a→g*), (*a→d*), and (*d→g*). | 1A.(*a→g*) 1B.(*a→g*) | 
|
| Date and Reference | Description | Key | Gel |
|---|---|---|---|
| 17th Aug 2007 (2-4) | PstI digested minipreps of (*m*) and (*s*) in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis. Gel 1. | 1.-3.(*m*)+4/6B (PstI site mutated) 4.-6.(*m*)+3/20G | 
|
| 17th Aug 2007 (2-4) | PstI digested minipreps of (*m*) and (*s*) in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis. Gel 2. | 1.-2.(*s*)+3/20G (1st PstI site mutated) 3.(*m*)+4/6B | 
|
| Date and Reference | Description | Key | Gel |
|---|---|---|---|
| 23rd Aug 2007 | (*s*) minipreps in 4/6B and 3/20G run after the second round of site-directed mutagenesis. | 1.-5.(*s*)+4/6B 6.-16.(*s*)+3/20G | 
|
| 23rd Aug 2007 | PstI digested (*s*) minipreps in 4/6B and 3/20G vectors after the second round of site-directed mutagenesis. | 1.-5.(*s*)+4/6B 6.-16.(*s*)+3/20G | 
|
| 23rd Aug 2007 | Colony PCR with "Pr_Prefix" and "XylR_Suffix" primers from Lynsey's transformations with Pr+XylR miniprep DNA. Gel 1. | 1.-7.colonies from the plate | 
|
| 23rd Aug 2007 | Colony PCR with "Pr_Prefix" and "XylR_Suffix" primers from Lynsey's transformations with Pr+XylR miniprep DNA. Gel 2. | 8.-12.colonies from the plate 13.neg control | 
|
