Glasgow/Wetlab/Week9
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| - | #[[User:MaijaP|Maija]] performed a PvuI digest for pDntA+3/20G and pDntA+4/6B constructs with Roche's PvuI enzyme. The bands | + | #[[User:MaijaP|Maija]] performed a PvuI digest for pDntA+3/20G and pDntA+4/6B constructs with Roche's PvuI enzyme. The bands were weird sizes, see the gel [[Glasgow/Wetlab/Gels#Monday 27th August 2007|here]]. |
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Revision as of 14:10, 29 August 2007
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Week 9
Monday 27th August 2007
- Maija performed a PvuI digest for pDntA+3/20G and pDntA+4/6B constructs with Roche's PvuI enzyme. The bands were weird sizes, see the gel here.
| Insert | Vector | Enzyme | Expected size | Result |
|---|---|---|---|---|
| pDntA | 3/20G | PvuI | 1450bp + 749bp | ~2500bp |
| pDntA | 4/6B | PvuI | 2375bp + 681bp | ~3800bp + ~1250bp |
Tuesday 28 August 2007
Wednesday 29th August 2007
Scott did colony PCR (see protocol 9) on the assembly transformations carried out yesterday using primers VF2 and VR. This will tell us if the BioBrick insert region is the expected (enlarged) size.
