Paris/August 30

From 2007.igem.org

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== Glycerol Stocks ==
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== Strains (glycerol stock) ==
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{|
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|- style="background: #ccccff;"
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! colspan="5" style="background: #ccccff;" | Strains glycerol stock
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|- style="background: #ccccff; text-align: center;"
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|width=5%| Number
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|width=25%| Name
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|width=55%| Description
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|width=5%| Verified clone
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|width=5%| Date
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |S46
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|MP15
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|Lambda Att B rev within pSB2K3
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|
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|[[Paris/August 22| August 22]]
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |S47
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|MP16
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|Lambda Att P within pSB2K3
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|
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|[[Paris/August 22| August 22]]
== Digestions ==
== Digestions ==

Revision as of 12:06, 30 August 2007

yesterday -- tomorrow

Contents

Expression test of DapA promotor by differential DAP repression

Plasmids: MiniPrep Products

Plasmids: MiniPrep Products
Number Name Description Products in use Date
MP15 Att B Sigma Att B rev within pSB2K3 August 30
MP16 Att P Sigma Att P within pSB2K3 August 30

Strains (glycerol stock)

Strains glycerol stock
Number Name Description Verified clone Date
S46 MP15 Lambda Att B rev within pSB2K3 August 22
S47 MP16 Lambda Att P within pSB2K3 August 22

Digestions

Growth kinetic on w121 strains

In order to precise the growth behavior/kinetics of our strain W121 in smaller DAP concentration, we fit those last ones in function of our last kinetics results (06/08/07)

Kinetic Array :w121 kinetic as a function of DAP and supplemented medium (S0.x)
1 2 3 4 5 6 7 8 9 10 11 12
A H20 H20 H20 H20 H20 H20 H20 H20 H20 H20 H20 H20
B H20 LB+0µM DAP LB+5µM DAP LB+8µM DAP LB+10µM DAP LB+12µM DAP LB+15µM DAP LB+17µM DAP LB+20µM DAP LB+30µM DAP LB+40µM DAP H20
C H20 S0.2+0µM DAP S0.2+5µM DAP S0.2+6µM DAP S0.2+7µM DAP S0.2+8µM DAP S0.2+9µM DAP S0.2+10µM DAP S0.2+15µM DAP S0.2+17µM DAP S0.2+20µM DAP H20
D H20 S0.4+0µM DAP S0.4+2µM DAP S0.4+3µM DAP S0.4+4µM DAP S0.4+5µM DAP S0.4+8µM DAP S0.4+10µM DAP S0.4+12µM DAP S0.4+15µM DAP S0.4+20µM DAP H20
E H20 S0.6+0µM DAP S0.6+1µM DAP S0.6+2µM DAP S0.6+3µM DAP S0.6+4µM DAP S0.6+5µM DAP S0.6+6µM DAP S0.6+7µM DAP S0.6+8µM DAP S0.6+10µM DAP H20
F H20 S0.8+0µM DAP S0.8+0,5µM DAP S0.8+1µM DAP S0.8+2µM DAP S0.8+3µM DAP S0.8+4µM DAP S0.8+5µM DAP S0.8+6µM DAP S0.8+7µM DAP S0.8+10µM DAP H20
G H20 H20 H20 H20 H20 H20 H20 H20 H20 H20 H20 H20
H H20 H20 H20 H20 H20 H20 H20 H20 H20 H20 H20 H20

The 96 well plate is surrounded by H2O in order to maintain humidity during the assay. In each slot, 200µL of the growth medium (LB or S0.x) is mixed with 2µL of w121 culture grown ON, and with different amount of DAP (see table). The growth profile is measured over a period of 20H, a DO measurement is acquired each 4min10s.

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