Paris/August 30
From 2007.igem.org
(Difference between revisions)
(→Plasmids: MiniPrep Products) |
(→Strains (glycerol stock)) |
||
| Line 42: | Line 42: | ||
|Lambda Att B rev within pSB2K3 | |Lambda Att B rev within pSB2K3 | ||
| | | | ||
| - | |[[Paris/August | + | |[[Paris/August 30| August 30]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |S47 | | style="background: #ccffcc;" |S47 | ||
| Line 48: | Line 48: | ||
|Lambda Att P within pSB2K3 | |Lambda Att P within pSB2K3 | ||
| | | | ||
| - | |[[Paris/August | + | |[[Paris/August 30| August 30]] |
|} | |} | ||
Revision as of 12:07, 30 August 2007
Contents |
Expression test of DapA promotor by differential DAP repression
Plasmids: MiniPrep Products
| Plasmids: MiniPrep Products | ||||
|---|---|---|---|---|
| Number | Name | Description | Products in use | Date |
| MP15 | Att B | Lambda Att B rev within pSB2K3 | August 30 | |
| MP16 | Att P | Lambda Att P within pSB2K3 | August 30 | |
Strains (glycerol stock)
| Strains glycerol stock | ||||
|---|---|---|---|---|
| Number | Name | Description | Verified clone | Date |
| S46 | MP15 | Lambda Att B rev within pSB2K3 | August 30 | |
| S47 | MP16 | Lambda Att P within pSB2K3 | August 30 | |
Digestions
Growth kinetic on w121 strains
In order to precise the growth behavior/kinetics of our strain W121 in smaller DAP concentration, we fit those last ones in function of our last kinetics results (06/08/07)
| Kinetic Array :w121 kinetic as a function of DAP and supplemented medium (S0.x) | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
| A | H20 | H20 | H20 | H20 | H20 | H20 | H20 | H20 | H20 | H20 | H20 | H20 |
| B | H20 | LB+0µM DAP | LB+5µM DAP | LB+8µM DAP | LB+10µM DAP | LB+12µM DAP | LB+15µM DAP | LB+17µM DAP | LB+20µM DAP | LB+30µM DAP | LB+40µM DAP | H20 |
| C | H20 | S0.2+0µM DAP | S0.2+5µM DAP | S0.2+6µM DAP | S0.2+7µM DAP | S0.2+8µM DAP | S0.2+9µM DAP | S0.2+10µM DAP | S0.2+15µM DAP | S0.2+17µM DAP | S0.2+20µM DAP | H20 |
| D | H20 | S0.4+0µM DAP | S0.4+2µM DAP | S0.4+3µM DAP | S0.4+4µM DAP | S0.4+5µM DAP | S0.4+8µM DAP | S0.4+10µM DAP | S0.4+12µM DAP | S0.4+15µM DAP | S0.4+20µM DAP | H20 |
| E | H20 | S0.6+0µM DAP | S0.6+1µM DAP | S0.6+2µM DAP | S0.6+3µM DAP | S0.6+4µM DAP | S0.6+5µM DAP | S0.6+6µM DAP | S0.6+7µM DAP | S0.6+8µM DAP | S0.6+10µM DAP | H20 |
| F | H20 | S0.8+0µM DAP | S0.8+0,5µM DAP | S0.8+1µM DAP | S0.8+2µM DAP | S0.8+3µM DAP | S0.8+4µM DAP | S0.8+5µM DAP | S0.8+6µM DAP | S0.8+7µM DAP | S0.8+10µM DAP | H20 |
| G | H20 | H20 | H20 | H20 | H20 | H20 | H20 | H20 | H20 | H20 | H20 | H20 |
| H | H20 | H20 | H20 | H20 | H20 | H20 | H20 | H20 | H20 | H20 | H20 | H20 |
The 96 well plate is surrounded by H2O in order to maintain humidity during the assay. In each slot, 200µL of the growth medium (LB or S0.x) is mixed with 2µL of w121 culture grown ON, and with different amount of DAP (see table). The growth profile is measured over a period of 20H, a DO measurement is acquired each 4min10s.
