Week 14
From 2007.igem.org
(Difference between revisions)
| Line 10: | Line 10: | ||
*Ligation for: | *Ligation for: | ||
::-[http://partsregistry.org/Part:I763004 I763004]+[http://partsregistry.org/Part:I763033 I763033]; | ::-[http://partsregistry.org/Part:I763004 I763004]+[http://partsregistry.org/Part:I763033 I763033]; | ||
| - | ::-[http://partsregistry.org/Part:I763012 I763012]+[http://partsregistry.org/Part:I763020 I763020] | + | ::-[http://partsregistry.org/Part:I763012 I763012]+[http://partsregistry.org/Part:I763020 I763020][http://partsregistry.org/Part:I763032 (I763032)]. |
| Line 22: | Line 22: | ||
::-[http://partsregistry.org/Part:pSB4A3 pSB4A3] with Eco/Pst1. | ::-[http://partsregistry.org/Part:pSB4A3 pSB4A3] with Eco/Pst1. | ||
*Run on electrophoresis gel. | *Run on electrophoresis gel. | ||
| - | *[http://partsregistry.org/Part:I763031 I763031], | + | *[http://partsregistry.org/Part:I763031 I763031], +[http://partsregistry.org/Part:I763036 I763036] and [http://partsregistry.org/Part:pSB4A3 pSB4A3] band extraction. |
*Transformation of | *Transformation of | ||
::-[http://partsregistry.org/Part:I763034 I763034]; | ::-[http://partsregistry.org/Part:I763034 I763034]; | ||
Revision as of 10:35, 8 October 2007
- 10/01/07
- Meeting: Ethical issue.
- 10/02/07
- Digestion of [http://partsregistry.org/Part:pSB4A3 pSB4A3];
- Run on electrophoresis gel.
- [http://partsregistry.org/Part:pSB4A3 pSB4A3]; and [http://partsregistry.org/Part:I763033 I763033] band extraction.
- Ligation for:
- -[http://partsregistry.org/Part:I763004 I763004]+[http://partsregistry.org/Part:I763033 I763033];
- -[http://partsregistry.org/Part:I763012 I763012]+[http://partsregistry.org/Part:I763020 I763020][http://partsregistry.org/Part:I763032 (I763032)].
- 10/03/07
- Digestion of:
- -[http://partsregistry.org/Part:I763032 I763032] with Eco/Pst1;
- -[http://partsregistry.org/Part:I763031 I763031] with Eco/Pst1;
- -[http://partsregistry.org/Part:I763036 I763036] with Eco/Pst1;
- -[http://partsregistry.org/Part:pSB4A3 pSB4A3] with Eco/Pst1.
- Run on electrophoresis gel.
- [http://partsregistry.org/Part:I763031 I763031], +[http://partsregistry.org/Part:I763036 I763036] and [http://partsregistry.org/Part:pSB4A3 pSB4A3] band extraction.
- Transformation of
- -[http://partsregistry.org/Part:I763034 I763034];
- -[http://partsregistry.org/Part:I763031 I763031];
- -[http://partsregistry.org/Part:I763032 I763032].
- Ligation for
- -[http://partsregistry.org/Part:I763031 I763031]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
- -[http://partsregistry.org/Part:I763032 I763032::-[http://partsregistry.org/Part:I763032 I763032].
Experimental Setup
- To have an higher precision for our measurements, we have decided to get, for the same field of view on the same sample of bacteria solution, either a short film segment (from which we can extract frames) and a Microsoft Excel file, which contains a ten seconds interval of values from the photomultiplier output, taken at a 100Hz frequency.
- In order to have the same focus for the microscope's oculars, the photocamera and the photomultiplier we have to calibrate our system by changing the position of both camera and photomultiplier.
- Finally, we check that the photomultiplier field of view is the same size as the microscope's one: by moving the appropiate diaphragms, we adjust the previously done settings.
- 10/04/07
- Trasformation of
- -[http://partsregistry.org/Part:I763031 I763031]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
- -[http://partsregistry.org/Part:I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
- We inoculate [http://partsregistry.org/Part:I763034 I763034],I763031+pSB4A3 and [http://partsregistry.org/Part:I763032 I763032].+pSB4A3 (in 6 ml).
- 10/05/07
- Stock preparation (500 ul) of I763034,I763031+pSB4A3 and I763032+pSB4A3.
- Miniprep of I763034,I763031+pSB4A3 and I763032+pSB4A3.
- Control digestion of
- -I763034 with Xba/Pst1;
- -I763031 with Eco/Xba and Xba/Pst1;
- -I763032 with Eco/Xba and Xba/Pst1.
- Run on electrophoresis gel and I763032 (Eco/Xba and Xba/Pst1) band extraction.
