Tokyo/sunaba
From 2007.igem.org
| Line 43: | Line 43: | ||
====For our circuit, we developped the [[Tokyo/Hybrid promoter|promoter]] that can receive two input,AHL & reperssor.==== | ====For our circuit, we developped the [[Tokyo/Hybrid promoter|promoter]] that can receive two input,AHL & reperssor.==== | ||
| + | <br>図 配列と棒グラフ | ||
| + | |||
<!--New part development:Hybrid promoterPBとして、2つの入力を受けるプロモータを開発する必要があった | <!--New part development:Hybrid promoterPBとして、2つの入力を受けるプロモータを開発する必要があった | ||
| - | |||
--> | --> | ||
|width="150px" valign="top" | | |width="150px" valign="top" | | ||
[[Image:Hybrid.JPG|200px|]] | [[Image:Hybrid.JPG|200px|]] | ||
|} | |} | ||
| - | |||
| - | |||
===2-1.Dry : [[Tokyo/Formulation |Formulation]]=== | ===2-1.Dry : [[Tokyo/Formulation |Formulation]]=== | ||
We wrote the Formulation | We wrote the Formulation | ||
| Line 64: | Line 63: | ||
--> | --> | ||
[[Image:hill AHL.JPG|200px]]|[[Image:hill IPTG.JPG|200px]] | [[Image:hill AHL.JPG|200px]]|[[Image:hill IPTG.JPG|200px]] | ||
| + | <br>ns=2.3 nb=2.7 | ||
===2-3.Dry : [[Tokyo/Simulation |Simulation]]=== | ===2-3.Dry : [[Tokyo/Simulation |Simulation]]=== | ||
Revision as of 09:31, 21 October 2007
砂場。
1,Project summaryConcept& model: Bacterial society that follows Pareto's principle like ant society
Requirements:
Genetic circuit with new part:
Simulation:using parameters from our wet experiments
Our construction parts are Here!!! |
2,WorksApproach:We alternated approach of Wet and Dry.2-0.Wet : Hybrid promoter
2-1.Dry : FormulationWe wrote the Formulation 2-2.Wet : AssayWe decided the parameter of hill coefficients by the assay of AHL & IPTG
2-3.Dry : Simulationwet実験で求められたパラメータを用いて、集団が怠け者の細胞のみとなった場合に、一度不安定化して、続いて働き者と怠け者になれることをStocastic Simulationで確認した。このシステムの挙動に必要な他のパラメータ範囲が判明した。
2-4.Wet : AssaySimulationを満たすための他のパラメータが現在のプラスミドで可能であるかを、大腸菌を用いた実験で確認した。
2-5.Future works3,About our team
Advisors: Last year's member
Instructors
Links sunaba
|
