Week 14

From 2007.igem.org

(Difference between revisions)
 
Line 61: Line 61:
-
[[Bologna | Back]]
+
[[Bologna#Diary | Back]]

Latest revision as of 15:51, 26 October 2007

10/01/07
  • Meeting: Ethical issue.
  • Work on image segmentation algorithm.


10/02/07
  • Digestion of [http://partsregistry.org/Part:pSB4A3 pSB4A3];
  • Run on electrophoresis gel.
  • [http://partsregistry.org/Part:pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763033 I763033] band extraction.
  • Ligations for:
-[http://partsregistry.org/Part:BBa_I763004 I763004]+[http://partsregistry.org/Part:BBa_I763033 I763033];
-[http://partsregistry.org/Part:BBa_I763012 I763012]+[http://partsregistry.org/Part:BBa_I763020 I763020][http://partsregistry.org/Part:BBa_I763032 (I763032)].



10/03/07
  • Digestions of:
-[http://partsregistry.org/Part:BBa_I763032 I763032] with Eco/Pst1;
-[http://partsregistry.org/Part:BBa_I763031 I763031] with Eco/Pst1;
-[http://partsregistry.org/Part:BBa_I763036 I763036] with Eco/Pst1;
-[http://partsregistry.org/Part:pSB4A3 pSB4A3] with Eco/Pst1.
  • Run on electrophoresis gel.
  • [http://partsregistry.org/Part:BBa_I763031 I763031], [http://partsregistry.org/Part:BBa_I763036 I763036] and [http://partsregistry.org/Part:pSB4A3 pSB4A3] band extraction.
  • Transformations of:
-[http://partsregistry.org/Part:BBa_I763034 I763034];
-[http://partsregistry.org/Part:BBa_I763031 I763031];
-[http://partsregistry.org/Part:BBa_I763032 I763032].
  • Ligations for:
-[http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
-[http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];

Experimental Setup

  • To have an higher precision for our measurements, we have decided to get, for the same field of view on the same sample of bacteria solution, either a short film segment (from which we can extract frames) and a Microsoft Excel file, which contains a ten seconds interval of values from the photomultiplier output, taken at a 100Hz frequency.
  • In order to have the same focus for the microscope's oculars, the photocamera and the photomultiplier we have to calibrate our system by changing the position of both camera and photomultiplier.
  • Finally, we check that the photomultiplier field of view is the same size as the microscope's one: by moving the appropiate diaphragms, we adjust the previously done settings.


10/04/07
  • Trasformation of:
-[http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
-[http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3];
  • We inoculate [http://partsregistry.org/Part:BBa_I763034 I763034], [http://partsregistry.org/Part:BBa_I763031 I763031] + [http://partsregistry.org/Part:pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:pSB4A3 pSB4A3] (in 6 ml).



10/05/07
  • Stock preparation (500 ul) of [http://partsregistry.org/Part:I763034 I763034],[http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part:BBa_pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:BBa_pSB4A3 pSB4A3].
  • Miniprep of [http://partsregistry.org/Part:BBa_I763034 I763034], [http://partsregistry.org/Part:BBa_I763031 I763031]+[http://partsregistry.org/Part:BBa_pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763032 I763032]+[http://partsregistry.org/Part:BBa_pSB4A3 pSB4A3].
  • Control digestion of:
-[http://partsregistry.org/Part:BBa_I763034 I763034] with Xba/Pst1;
-[http://partsregistry.org/Part:BBa_I763031 I763031] with Eco/Xba and Xba/Pst1;
-[http://partsregistry.org/Part:BBa_I763032 I763032] with Eco/Xba and Xba/Pst1.
  • Run on electrophoresis gel and [http://partsregistry.org/Part:BBa_I763032 I763032] (Eco/Xba and Xba/Pst1) band extraction.


Back

Retrieved from "http://2007.igem.org/wiki/index.php/Week_14"