Edinburgh/Disclaimer
From 2007.igem.org
(Difference between revisions)
| Line 6: | Line 6: | ||
*# RT-PCR of COMT from alfalfa was attempted by '''Lucy Montgomery''', an undergraduate Honours student who worked with the team for a part of the project period. | *# RT-PCR of COMT from alfalfa was attempted by '''Lucy Montgomery''', an undergraduate Honours student who worked with the team for a part of the project period. | ||
*# Attempts to transform ''Lactobacillus'' and ''Streptococcus'' (unsuccessful) and ''Bacillus'' (successful) with pTG262-RFP were performed mainly by '''Chris French''' (Instructor). | *# Attempts to transform ''Lactobacillus'' and ''Streptococcus'' (unsuccessful) and ''Bacillus'' (successful) with pTG262-RFP were performed mainly by '''Chris French''' (Instructor). | ||
| - | *# Mutation of the PstI sites in the crtIB BioBrick,assembly of the crtEIB, lacZ-sam8 and sam8- | + | *# Mutation of the PstI sites in the crtIB BioBrick,assembly of the ''crtEIB'', ''lacZ''-''sam8'' and ''sam8''-''sam''5 composite BioBricks,and preliminary TLC experiments to detect vanillin pathway intermediates, were performed mainly by '''Chris French''' (Instructor). |
*# Initial fluorescence microscopy was performed by a colleague of '''Alistair Elfick''' (Instructor). | *# Initial fluorescence microscopy was performed by a colleague of '''Alistair Elfick''' (Instructor). | ||
| Line 14: | Line 14: | ||
* '''Prof. Millicent Masters''', University of Edinburgh: for single copy vector pHR277. | * '''Prof. Millicent Masters''', University of Edinburgh: for single copy vector pHR277. | ||
* '''Dr. Mike Gasson''' and '''Dr. Claire Shearman''', Institute of Food Research, Norwich: for Gram positive vector pTG262. | * '''Dr. Mike Gasson''' and '''Dr. Claire Shearman''', Institute of Food Research, Norwich: for Gram positive vector pTG262. | ||
| - | * '''Dr. Anton Linnenbrink''', Albert-Ludwigs-Universitat, Freiburg: for supplying DNA encoding sam8 and sam5. | + | * '''Dr. Anton Linnenbrink''', Albert-Ludwigs-Universitat, Freiburg: for supplying DNA encoding ''sam8'' and ''sam5''. |
Revision as of 19:13, 26 October 2007
The Edinburgh iGEM 2007 Team declares that:
- Both projects were conceived and designed by the student team members within the time course of the project.
- All modelling and design work was performed by the students.
- All laboratory work was performed by the students with the following exceptions:
- RT-PCR of COMT from alfalfa was attempted by Lucy Montgomery, an undergraduate Honours student who worked with the team for a part of the project period.
- Attempts to transform Lactobacillus and Streptococcus (unsuccessful) and Bacillus (successful) with pTG262-RFP were performed mainly by Chris French (Instructor).
- Mutation of the PstI sites in the crtIB BioBrick,assembly of the crtEIB, lacZ-sam8 and sam8-sam5 composite BioBricks,and preliminary TLC experiments to detect vanillin pathway intermediates, were performed mainly by Chris French (Instructor).
- Initial fluorescence microscopy was performed by a colleague of Alistair Elfick (Instructor).
In addition to our sponsors, listed above, we also gratefully acknowledge the assistance of:
- Dr. Garry Blakely, University of Edinburgh: advice on recombinases.
- Prof. Millicent Masters, University of Edinburgh: for single copy vector pHR277.
- Dr. Mike Gasson and Dr. Claire Shearman, Institute of Food Research, Norwich: for Gram positive vector pTG262.
- Dr. Anton Linnenbrink, Albert-Ludwigs-Universitat, Freiburg: for supplying DNA encoding sam8 and sam5.
