From 2007.igem.org

Revision as of 16:23, 19 July 2007 (view source) MaijaP (Talk | contribs) (→03/07) ← Older edit		Revision as of 16:27, 19 July 2007 (view source) MaijaP (Talk | contribs) (→04/07) Newer edit →
Line 10:		Line 10:
	=== 04/07 ===		=== 04/07 ===
-	1 All wetlab researched BioBricks.	+	#All wetlab researched BioBricks.
-	2 Reporter constructs and mini-Tn5 stocks looked out.	+	#Reporter constructs and mini-Tn5 stocks looked out.
-	3 Streaked the following: P. putida PAW 340 pJAK14 Carb. plate	+	#Streaked the following:
-	Tn5 lux AB Carb. plate	+	##P. putida PAW 340 pJAK14 Carb. plate
-	Mini-Tn5 lux AB Carb. plate	+	##Tn5 lux AB Carb. plate
-	P. Fluorescens NCIMB 9815 Carb. plate	+	##Mini-Tn5 lux AB Carb. plate
-	P. putida KT2440 LB	+	##P. Fluorescens NCIMB 9815 Carb. plate
-		+	##P. putida KT2440 LB
-	JM109 pBluescript 5k+ Carb. plate	+	##JM109 pBluescript 5k+ Carb. plate
-	Mini-Tn5 Tc Carb. Plate	+	##Mini-Tn5 Tc Carb. Plate
-	pQF52 Carb. plate	+	##pQF52 Carb. plate
-	P. Fluorescens 9815 LB	+	##P. Fluorescens 9815 LB
-	E. coli pJAK14 Km plate	+	##E. coli pJAK14 Km plate
-		+	##Il DntR in pOF52 Carb. plate
-	Il DntR in pOF52 Carb. plate	+	##pUCINR in Ω strain C Carb. plate
-	pUCINR in Ω strain C Carb. plate	+	##pGLTUR Carb. plate
-	pGLTUR Carb. plate	+	##Mini-Tn5 Kan Carb. plate
-	Mini-Tn5 Kan Carb. plate	+	##Mini-Tn5 Sm/Sp Carb. plate
-	Mini-Tn5 Sm/Sp Carb. plate	+	##Mini-Tn5 1cc2 Carb. plate
-		+	##E. coli Sa1 LB
-	Mini-Tn5 1cc2 Carb. plate	+	##DmpR #24 Carb. plate
-	E. coli Sa1 LB	+	##Mini-Tn5 lac 32 in E. coli 517 Carb. plate
-	DmpR #24 Carb. plate	+	##Mini-Tn5 Tc Carb. Plate
-	Mini-Tn5 lac 32 in E. coli 517 Carb. plate	+	##Mini-Tn5 Cm Carb. plate
-	Mini-Tn5 Tc Carb. Plate	+	##DmpR WT Carb. plate
-		+	##E.coli sm 10 pESD15 Tn5 GFP Carb. plate
-	Mini-Tn5 Cm Carb. plate	+	##pUJ8 Carb. Plate
-	DmpR WT Carb. plate	+
-	E.coli sm 10 pESD15 Tn5 GFP Carb. plate	+
-	pUJ8 Carb. Plate	+

---

Revision as of 16:27, 19 July 2007

PROTOCOLSREFERENCES

Week 1

03/07

Maija and Christine prepared LB broth and LB agar with Protocol 1. Made 4 batches of plates, 2 with kanamycin and 2 with carbenicillin.

04/07

1. All wetlab researched BioBricks.
2. Reporter constructs and mini-Tn5 stocks looked out.
3. Streaked the following:
   1. P. putida PAW 340 pJAK14 Carb. plate
   2. Tn5 lux AB Carb. plate
   3. Mini-Tn5 lux AB Carb. plate
   4. P. Fluorescens NCIMB 9815 Carb. plate
   5. P. putida KT2440 LB
   6. JM109 pBluescript 5k+ Carb. plate
   7. Mini-Tn5 Tc Carb. Plate
   8. pQF52 Carb. plate
   9. P. Fluorescens 9815 LB
   10. E. coli pJAK14 Km plate
   11. Il DntR in pOF52 Carb. plate
   12. pUCINR in Ω strain C Carb. plate
   13. pGLTUR Carb. plate
   14. Mini-Tn5 Kan Carb. plate
   15. Mini-Tn5 Sm/Sp Carb. plate
   16. Mini-Tn5 1cc2 Carb. plate
   17. E. coli Sa1 LB
   18. DmpR #24 Carb. plate
   19. Mini-Tn5 lac 32 in E. coli 517 Carb. plate
   20. Mini-Tn5 Tc Carb. Plate
   21. Mini-Tn5 Cm Carb. plate
   22. DmpR WT Carb. plate
   23. E.coli sm 10 pESD15 Tn5 GFP Carb. plate
   24. pUJ8 Carb. Plate