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	#Reporter constructs and mini-Tn5 stocks looked out.		#Reporter constructs and mini-Tn5 stocks looked out.
	#Streaked the following:		#Streaked the following:
-	***P. putida PAW 340 pJAK14 Carb. plate	+	##P. putida PAW 340 pJAK14 (Carb. plate)
-	##Tn5 lux AB Carb. plate	+	##Tn5 lux AB (Carb. plate)
-	##Mini-Tn5 lux AB Carb. plate	+	##Mini-Tn5 lux AB (Carb. plate)
-	##P. Fluorescens NCIMB 9815 Carb. plate	+	##P. Fluorescens NCIMB 9815 (Carb. plate)
-	##P. putida KT2440 LB	+	##P. putida KT2440 (LB)
-	##JM109 pBluescript 5k+ Carb. plate	+	##JM109 pBluescript 5k+ (Carb. plate)
-	##Mini-Tn5 Tc Carb. Plate	+	##Mini-Tn5 Tc (Carb. Plate)
-	##pQF52 Carb. plate	+	##pQF52 (Carb. plate)
-	##P. Fluorescens 9815 LB	+	##P. Fluorescens 9815 (LB)
-	##E. coli pJAK14 Km plate	+	##E. coli pJAK14 (Km plate)
-	##Il DntR in pOF52 Carb. plate	+	##Il DntR in pOF52 (Carb. plate)
-	##pUCINR in Ω strain C Carb. plate	+	##pUCINR in Ω strain C (Carb. plate)
-	##pGLTUR Carb. plate	+	##pGLTUR (Carb. plate)
-	##Mini-Tn5 Kan Carb. plate	+	##Mini-Tn5 Kan (Carb. plate)
-	##Mini-Tn5 Sm/Sp Carb. plate	+	##Mini-Tn5 Sm/Sp (Carb. plate)
-	##Mini-Tn5 1cc2 Carb. plate	+	##Mini-Tn5 1cc2 (Carb. plate)
-	##E. coli Sa1 LB	+	##E. coli Sa1 (LB)
-	##DmpR #24 Carb. plate	+	##DmpR #24 (Carb. plate)
-	##Mini-Tn5 lac 32 in E. coli 517 Carb. plate	+	##Mini-Tn5 lac 32 in E. coli 517 (Carb. plate)
-	##Mini-Tn5 Tc Carb. Plate	+	##Mini-Tn5 Tc (Carb. Plate)
-	##Mini-Tn5 Cm Carb. plate	+	##Mini-Tn5 Cm (Carb. plate)
-	##DmpR WT Carb. plate	+	##DmpR WT (Carb. plate)
-	##E.coli sm 10 pESD15 Tn5 GFP Carb. plate	+	##E.coli sm 10 pESD15 Tn5 GFP (Carb. plate)
-	##pUJ8 Carb. Plate	+	##pUJ8 (Carb. Plate)

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Revision as of 16:32, 19 July 2007

PROTOCOLSREFERENCES

Week 1

03/07

1. Maija and Christine prepared LB broth and LB agar with Protocol 1. Made 4 batches of plates, 2 with kanamycin and 2 with carbenicillin.

04/07

1. All wetlab researched BioBricks.
2. Reporter constructs and mini-Tn5 stocks looked out.
3. Streaked the following:
   1. P. putida PAW 340 pJAK14 (Carb. plate)
   2. Tn5 lux AB (Carb. plate)
   3. Mini-Tn5 lux AB (Carb. plate)
   4. P. Fluorescens NCIMB 9815 (Carb. plate)
   5. P. putida KT2440 (LB)
   6. JM109 pBluescript 5k+ (Carb. plate)
   7. Mini-Tn5 Tc (Carb. Plate)
   8. pQF52 (Carb. plate)
   9. P. Fluorescens 9815 (LB)
   10. E. coli pJAK14 (Km plate)
   11. Il DntR in pOF52 (Carb. plate)
   12. pUCINR in Ω strain C (Carb. plate)
   13. pGLTUR (Carb. plate)
   14. Mini-Tn5 Kan (Carb. plate)
   15. Mini-Tn5 Sm/Sp (Carb. plate)
   16. Mini-Tn5 1cc2 (Carb. plate)
   17. E. coli Sa1 (LB)
   18. DmpR #24 (Carb. plate)
   19. Mini-Tn5 lac 32 in E. coli 517 (Carb. plate)
   20. Mini-Tn5 Tc (Carb. Plate)
   21. Mini-Tn5 Cm (Carb. plate)
   22. DmpR WT (Carb. plate)
   23. E.coli sm 10 pESD15 Tn5 GFP (Carb. plate)
   24. pUJ8 (Carb. Plate)