From 2007.igem.org
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| - | '''Products and Parts'''
| + | ===Hop Count with Conjugation=== |
| | + | Project Proposal |
| | + | Project Presentation |
| | + | Road Map |
| | + | Related Articles |
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| - | '''Procedures'''<br>
| + | ===Push-on-push-off Switch=== |
| - | [[iGEM:Peking/2007/Count-Conjugation-Procedure |Hop-count with Conjugation]]<br>
| + | Project proposal |
| - | [[iGEM:Peking/2007/Switch-Procedure |Push-on-push-off Switch]]<br>
| + | Project presentation |
| - | | + | Road Map |
| - | '''Protocols'''
| + | Related Articles |
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| - | [[iGEM:Peking/2007/Switch:Miller|Switch:Miller Method]]<br>
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| - | [[iGEM:Peking/2007/Oligos | Oligonucleotides]]<br>
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| - | [[iGEM:Peking/2007/Sequencing | Sequencing]]<br>
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| - | [[iGEM:Peking/2007/Transformation | Transformation]]<br>
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| - | [[iGEM:Peking/2007/Digestion | Enzymatic DNA Digestion]]<br>
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| - | [[iGEM:Peking/2007/Count:Miniprep | Count:Miniprep]]<br>
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| - | [[iGEM:Peking/2007/Count:Gel-Extraction | Count:DNA Gel Extraction]]<br>
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| - | [[iGEM:Peking/2007/Count:PCR product purifcation| Count:PCR product purifcation]]<br>
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| - | [[iGEM:Peking/2007/Count:Preparation of Competent Cells | Count:Preparation of Competent Cells]]<br>
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| - | [[iGEM:Peking/2007/Count:Conjugation| Count:Conjugation test]]<br>
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| - | [[iGEM:Peking/2007/Count:Agarose Gel Electrophoresis| Count:Agarose Gel Electrophoresis]]<br>
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| - | [[iGEM:Peking/2007/Ligation | Ligation]]<br>
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| - | '''Notebooks'''<br>
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| - | [[iGEM:Peking/2007/Count-Conjugation-Notebook |Hop-count with Conjugation]]<br>
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| - | [[iGEM:Peking/2007/Switch_Notebook|Push-on-push-off Switch]]<br>
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| - | [[iGEM:Peking/2007/Turtle_Diary|Turtle Diary]]<br>
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Revision as of 05:53, 8 August 2007
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There are
days left until the Jamboree!
Hop Count with Conjugation
Hop counting is a cell-cell communication system in which the signals transfered among the cells record the number of times of transfer. We decided to use conjugation as our one to one cell-cell communication system and plasmids as the vector for the transfered signal.
With the rolling-cycle replication of conjugation, independent tandem oriT (BBa_J01002 and more) pairs can be inserted into the same plasmids, losing one tandem region at each single conjugation event.
Sequential conjugation and deletion of the different oriT pairs can be achieved by fine control of the expression of the cis-acting elements of the independent conjugation systems.
Besides intellectual interests, these systems are the core parts of controllable and encoded local cell-cell communication, which can be used in potential pattern formation and material weaving applications.
Push-on-push-off Switch
We propose to construct a gene regulatory circuit in E. coli which functions as a push-on/push-off switch. The immediate output of such circuit is determined by the input signal and the internal state of the circuit. It is designed to response to low dose UV irradiation. Under intermittent irradiation, two reporter genes are expected to be induced alternately.
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Team
Hop Count with Conjugation
Project Proposal
Project Presentation
Road Map
Related Articles
Push_on_push_off Switch
Project proposal
Project presentation
Road Map
Related Articles
Meetings
Brainstorming
Some of our ideas are listed here
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Project
Hop Count with Conjugation
Project Proposal
Project Presentation
Road Map
Related Articles
Push-on-push-off Switch
Project proposal
Project presentation
Road Map
Related Articles
|
People
The iGEM 2007 PKU Team now consists of about 20 students and several faculty members from School of Life Science and Center of Theoretical Biology.
Students
Please sign up here.
Advisors
- [http://www.bio.pku.edu.cn/collegereview/scholarinfo.jsp?name=wangyp Wang Yiping]
- Li Fangting
- [http://ctb.pku.edu.cn/main/cn/graduation/graduation-daoshijianjie-ouyangqi.htm Ouyang Qi]
- [http://linux2.ipc.pku.edu.cn/ Lai Luhua]
Photo
The Team
Our Campus
The Labs
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Email us at igem-pku AT googlegroups DOT com
External links iGEM | [http://www.igem2006.com iGEM 2006] | [http://partsregistry.org Registry of Standard Biological Parts] | [http://openwetware.org/wiki/IGEM:Peking/2007 iGEM PKU OWW Site] | iGEM 2007 Team List
