Glasgow/Wetlab/Gels
From 2007.igem.org
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| - | |[[Glasgow/Wetlab/Week6#Friday 10th August 2007| | + | |[[Glasgow/Wetlab/Week6#Friday 10th August 2007|10th Aug 2007 (2)]] |
|Colony PCR after ligating (*m*), (*s*), DntR and Pu with the construction vectors 3/20G and 4/6B. | |Colony PCR after ligating (*m*), (*s*), DntR and Pu with the construction vectors 3/20G and 4/6B. | ||
|1.(*m*)<br> | |1.(*m*)<br> | ||
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|[[Image:10thAug gel 1.JPG|200px]] | |[[Image:10thAug gel 1.JPG|200px]] | ||
|- | |- | ||
| - | |[[Glasgow/Wetlab/Week6#Friday 10th August 2007| | + | |[[Glasgow/Wetlab/Week6#Friday 10th August 2007|10th Aug 2007 (2)]] |
|Colony PCR after ligating (*m*), (*s*), DntR and Pu with the construction vectors 3/20G and 4/6B. | |Colony PCR after ligating (*m*), (*s*), DntR and Pu with the construction vectors 3/20G and 4/6B. | ||
|1.(*s*)<br> | |1.(*s*)<br> | ||
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|[[Image:10thAug gel 2.JPG|200px]] | |[[Image:10thAug gel 2.JPG|200px]] | ||
|- | |- | ||
| - | |[[Glasgow/Wetlab/Week7#Monday 13th August 2007| | + | |[[Glasgow/Wetlab/Week7#Monday 13th August 2007|13th Aug 2007 (4)]] |
|PCR of genes (*a→g*), (*a→d*), and (*d→g*). | |PCR of genes (*a→g*), (*a→d*), and (*d→g*). | ||
|1A:(*a→g*) <br> | |1A:(*a→g*) <br> | ||
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|[[Image:2007-08-13 12hr 43min.JPG|200px]] | |[[Image:2007-08-13 12hr 43min.JPG|200px]] | ||
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| - | |[[Glasgow/Wetlab/Week7#Friday 17th August 2007| | + | |[[Glasgow/Wetlab/Week7#Friday 17th August 2007|17th Aug 2007 (2-4)]] |
|PstI digested minipreps of (*m*) and (*s*) in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis. | |PstI digested minipreps of (*m*) and (*s*) in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis. | ||
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|[[Image:17thAug SDM1 PstI digest gel 1.JPG|200px]] | |[[Image:17thAug SDM1 PstI digest gel 1.JPG|200px]] | ||
|- | |- | ||
| - | |[[Glasgow/Wetlab/Week7#Friday 17th August 2007| | + | |[[Glasgow/Wetlab/Week7#Friday 17th August 2007|17th Aug 2007 (2-4)]] |
|PstI digested minipreps of (*m*) and (*s*) in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis. | |PstI digested minipreps of (*m*) and (*s*) in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis. | ||
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Revision as of 14:33, 28 August 2007
Glasgow Main Page | Back To Wetlab Log
Gels
Gels are labelled with numbers for rows and letters for columns.
| Date and Reference | Description | Key | Gel |
|---|---|---|---|
| 10th Aug 2007 (2) | Colony PCR after ligating (*m*), (*s*), DntR and Pu with the construction vectors 3/20G and 4/6B. | 1.(*m*) 2.(*m*) | 
|
| 10th Aug 2007 (2) | Colony PCR after ligating (*m*), (*s*), DntR and Pu with the construction vectors 3/20G and 4/6B. | 1.(*s*) 2.(*s*) | 
|
| 13th Aug 2007 (4) | PCR of genes (*a→g*), (*a→d*), and (*d→g*). | 1A:(*a→g*) 1B:(*a→g*) | 
|
| 17th Aug 2007 (2-4) | PstI digested minipreps of (*m*) and (*s*) in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis. | 
| |
| 17th Aug 2007 (2-4) | PstI digested minipreps of (*m*) and (*s*) in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis. | 
|
