Glasgow/Wetlab/Gels
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|[[Glasgow/Wetlab/Week7#Friday 17th August 2007|17th Aug 2007 (2-4)]] | |[[Glasgow/Wetlab/Week7#Friday 17th August 2007|17th Aug 2007 (2-4)]] | ||
|PstI digested minipreps of (*m*) and (*s*) in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis. | |PstI digested minipreps of (*m*) and (*s*) in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis. | ||
| - | | | + | |1.-3.(*m*)+4/6B (PstI site mutated)<br> |
| + | 4.-6.(*m*)+3/20G (PstI site mutated)<br> | ||
| + | 7.-9.(*s*)+4/6B (1st PstI site mutated)<br> | ||
| + | 10.-12.(*s*)+3/20G (2nd PstI site mutated)<br> | ||
| + | 13.-15.(*s*)+4/6B (2nd PstI site mutated)<br> | ||
| + | 16.(*s*)+3/20G (1st PstI site mutated)<br> | ||
|[[Image:17thAug SDM1 PstI digest gel 1.JPG|200px]] | |[[Image:17thAug SDM1 PstI digest gel 1.JPG|200px]] | ||
|- | |- | ||
|[[Glasgow/Wetlab/Week7#Friday 17th August 2007|17th Aug 2007 (2-4)]] | |[[Glasgow/Wetlab/Week7#Friday 17th August 2007|17th Aug 2007 (2-4)]] | ||
|PstI digested minipreps of (*m*) and (*s*) in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis. | |PstI digested minipreps of (*m*) and (*s*) in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis. | ||
| - | | | + | |1.-2. |
| + | 3. | ||
| + | 4. | ||
| + | 5. | ||
| + | 6. | ||
|[[Image:17thAug SDM1 PstI digest gel 2.JPG|200px]] | |[[Image:17thAug SDM1 PstI digest gel 2.JPG|200px]] | ||
|} | |} | ||
<br> | <br> | ||
Revision as of 14:41, 28 August 2007
Glasgow Main Page | Back To Wetlab Log
Gels
Gels are labelled with numbers for rows and letters for columns.
| Date and Reference | Description | Key | Gel |
|---|---|---|---|
| 10th Aug 2007 (2) | Colony PCR after ligating (*m*), (*s*), DntR and Pu with the construction vectors 3/20G and 4/6B. | 1.(*m*) 2.(*m*) | 
|
| 10th Aug 2007 (2) | Colony PCR after ligating (*m*), (*s*), DntR and Pu with the construction vectors 3/20G and 4/6B. | 1.(*s*) 2.(*s*) | 
|
| 13th Aug 2007 (4) | PCR of genes (*a→g*), (*a→d*), and (*d→g*). | 1A:(*a→g*) 1B:(*a→g*) | 
|
| 17th Aug 2007 (2-4) | PstI digested minipreps of (*m*) and (*s*) in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis. | 1.-3.(*m*)+4/6B (PstI site mutated) 4.-6.(*m*)+3/20G (PstI site mutated) | 
|
| 17th Aug 2007 (2-4) | PstI digested minipreps of (*m*) and (*s*) in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis. | 1.-2.
3. 4. 5. 6. | 
|
